Draft Genome Sequences of Six Type Strains of the Genus
            <i>Massilia</i>

Miess, Henrike; Frediansyah, Andri; Göker, Markus; Gross, Harald

doi:10.1128/mra.00226-20

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…Recently, the antibacterial activity of Massilia species via soluble compounds was described against two Gram-negative pathogens (Dahal et al 2021). Numerous biosynthetic gene clusters found in Massilia species suggesting them as a source for novel antimicrobial compounds (Miess et al 2020). Our results expand the knowledge about Massilia's antimicrobial potential to volatile emitted compounds.…”

Section: Discussionsupporting

confidence: 59%

Antimicrobial volatiles emitted by members of the nest microbiome of social spiders

Lammers

Nazipi

Zweers

et al. 2022

FEMS Microbiology Letters

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Microbes produce and respond to a range of structurally and functionally diverse volatiles. Many microbial volatiles have antimicrobial properties. Since volatiles can diffuse through complex three-dimensional systems like spider nests they are promising pathogen protection for social arthropods. Here, we analyzed the volatilomes of five nest microbiome members of the Namibian, social spider Stegodyphus dumicola, namely the bacteria Massilia sp. IC2-278, Massilia sp. IC2-477, Sphingomonas sp. IC-11, Streptomyces sp. IC-207, and the fungus Aureobasidium sp. CE_32, and tested their antimicrobial activity against two putative spider pathogens, namely Bacillus thuringiensis and Purpureocillium lilacinum. Most nest microbiome members released volatilomes with antibacterial and/or antifungal activities under in vitro conditions. The analysis of their volatilomes using GC/Q-TOF revealed that they include numerous antimicrobial volatiles. We tested the antimicrobial activity of five pure volatile compounds found in the volatilomes and revealed that all of them were antibacterial and/or antifungal. We could not identify the same antimicrobial volatiles as in a previous in situ study, but our results indicate that social spider-associated microorganisms as a source of antimicrobial volatiles are important for pathogen inhibition. Additionally, we showed the influence of the volatilomes on the antibiotic sensitivity of B. thuringiensis offering novel approaches to counter antibiotic resistance.

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Section: Discussionsupporting

confidence: 59%

Antimicrobial volatiles emitted by members of the nest microbiome of social spiders

Lammers

Nazipi

Zweers

et al. 2022

FEMS Microbiology Letters

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“…The β-proteobacterial genus Massilia was recently identified as an untapped reservoir of novel natural products [ 15 ]. In particular, the strain Massilia sp.…”

Section: Introductionmentioning

confidence: 99%

Intermediates and shunt products of massiliachelin biosynthesis in Massilia sp. NR 4-1

Steinmetz¹,

Lombe²,

Nett³

2023

Beilstein J. Org. Chem.

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Siderophores are small molecules secreted by microorganisms in order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced by Massilia sp. NR 4-1 under iron-deficient conditions. Based on experimental evidence and genome analysis, it was suspected that this bacterium synthesizes further iron-chelating molecules. After a thorough inspection of its metabolic profile, six previously overlooked compounds were isolated that were active in the chrome azurol S (CAS) assay. Mass spectrometric measurements and nuclear magnetic resonance spectroscopic analyses identified these compounds as possible biosynthetic intermediates or shunt products of massiliachelin. Their bioactivity was tested against one Gram-positive and three Gram-negative bacteria.

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“…Violacein and deoxyviolacein are known to be produced by various Gram-negative bacteria, including from the genus Chromobacterium [22], Collimonas [23], Duganella [14], Massilia [24], Pseudoalteromonas [19,25], and Janthinobacterium [26]. The production of these compounds varies between species, and previous works showed that media composition [27,28] and culture conditions [27,29] could significantly affect violacein production in different bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

Fermentation in Minimal Media and Fungal Elicitation Enhance Violacein and Deoxyviolacein Production in Two Janthinobacterium Strains

Frediansyah¹,

Manuhara

Kristanti

et al. 2022

Fermentation

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Violacein and its biosynthesis by-product deoxyviolacein are valuable natural pigments with different biological activities. Various efforts have been made to enhance violacein and deoxyviolacein production in microbes. However, the effect of different culture media, agitation, and fungal elicitation on biosynthesis in Janthinobacterium has not been evaluated. In this study, the effect of eight different culture media, agitation, and fungal elicitation by Agaricus bisporus on violacein and deoxviolacein production in Janthinobacterium agaricidamnosum DSM 9628 and Janthinobacterium lividum DSM 1552 were examined. The results showed that violacein and deoxviolacein are produced at high-levels when Janthinobacterium is cultivated in minimal media such as Davis minimal broth with glycerol (DMBgly), shipworm basal medium (SBM), and MM9 media. A 50-fold increase was observed in violacein production when Janthinobacterium was cultivated in these media compared to cultivation in Luria–Bertani (LB), nutrient broth (NB), and King’s B (KB). Agitation reduces violacein and deoxyviolacein production, while fungal elicitation decreases violacein but increases deoxyviolacein when Janthinobacterium is cultured in KB media, SBM, and modified SBM (MSBM). An antibacterial assay using various pathogenic bacteria showed that violacein and deoxyviolacein extracted from Janthinobacterium are effective against both Gram-positive and Gram-negative pathogens, confirming their functionality as antibacterial agents. The findings suggest that cultivation in minimal media and fungal elicitation might invoke a stress response, enhancing the production of violacein and deoxviolacein in Janthinobacterium.

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Draft Genome Sequences of Six Type Strains of the Genus Massilia

Abstract: High-quality draft genome sequences were determined for 6 Massilia sp. type strains. The genomes of these strains show considerable biosynthetic potential for producing secondary metabolites.

Cited by 8 publications

References 39 publications

Antimicrobial volatiles emitted by members of the nest microbiome of social spiders

Antimicrobial volatiles emitted by members of the nest microbiome of social spiders

Intermediates and shunt products of massiliachelin biosynthesis in Massilia sp. NR 4-1

Fermentation in Minimal Media and Fungal Elicitation Enhance Violacein and Deoxyviolacein Production in Two Janthinobacterium Strains

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