Draft Genome Sequences of Two
            <i>Sphingobium</i>
            Species Associated with Hexachlorocyclohexane (HCH) Degradation Isolated from an HCH-Contaminated Soil

Khan, Nelson; Toscan, Rodolfo Brizola; Lunayo, Accadius; Wamalwa, Benson; Muge, Edward K.; Mulaa, Francis; Kallies, René; Harms, Hauke; Wick, Lukas Y.; Rocha, Ulisses Nunes da

doi:10.1128/mra.00886-21

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Such an assumption is further supported by the fact that Sphingobium sp. S8 [ 37 ] and Fusarium equiseti K3 [ 36 ] had been isolated from the same organochloride pesticide-contaminated soil.…”

Section: Discussionmentioning

confidence: 99%

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Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

et al. 2023

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Biotransformation of soil organochlorine pesticides (OCP) is often impeded by a lack of nutrients relevant for bacterial growth and/or co-metabolic OCP biotransformation. By providing space-filling mycelia, fungi promote contaminant biodegradation by facilitating bacterial dispersal and the mobilization and release of nutrients in the mycosphere. We here tested whether mycelial nutrient transfer from nutrient-rich to nutrient-deprived areas facilitates bacterial OCP degradation in a nutrient-deficient habitat. The legacy pesticide hexachlorocyclohexane (HCH), a non-HCH-degrading fungus (Fusarium equiseti K3), and a co-metabolically HCH-degrading bacterium (Sphingobium sp. S8) isolated from the same HCH-contaminated soil were used in spatially structured model ecosystems. Using 13C-labeled fungal biomass and protein-based stable isotope probing (protein-SIP), we traced the incorporation of 13C fungal metabolites into bacterial proteins while simultaneously determining the biotransformation of the HCH isomers. The relative isotope abundance (RIA, 7.1–14.2%), labeling ratio (LR, 0.13–0.35), and the shape of isotopic mass distribution profiles of bacterial peptides indicated the transfer of 13C-labeled fungal metabolites into bacterial proteins. Distinct 13C incorporation into the haloalkane dehalogenase (linB) and 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (LinC), as key enzymes in metabolic HCH degradation, underpin the role of mycelial nutrient transport and fungal-bacterial interactions for co-metabolic bacterial HCH degradation in heterogeneous habitats. Nutrient uptake from mycelia increased HCH removal by twofold as compared to bacterial monocultures. Fungal-bacterial interactions hence may play an important role in the co-metabolic biotransformation of OCP or recalcitrant micropollutants (MPs).

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Section: Discussionmentioning

confidence: 99%

“…The HCH degrading bacterium Sphingobium sp. S8 was isolated from the same soil as the fungus and its draft genome sequence was described by Khan et al [ 37 ]. The bacterium was cultivated on Luria-Bertani (LB) (DSMZ medium 381) agar medium treated with γ-HCH (97%; Sigma) to a final concentration of 100 µg mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

et al. 2023

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show abstract

“…The HCH degrading bacterium Sphingobium sp. S8 was isolated from the same soil as the fungus and its draft genome sequence was described by Khan et al (37). The bacterium was cultivated on Luria-Bertani (LB) (DSMZ) agar medium treated with γ-HCH (97%; Sigma) to a final concentration of 100 μg/ml.…”

Section: Methodsmentioning

confidence: 99%

Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

Khan

Muge

Mulaa

et al. 2022

Preprint

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Biotransformation of soil organochlorine pesticides (OCP) is often impeded by a lack of nutrients relevant for bacterial growth and/or co-metabolic OCP biotransformation. By providing space-filling mycelia, fungi promote contaminant biodegradation by facilitating bacterial dispersal and the mobilization and release of nutrients in the mycosphere. We here tested whether mycelial nutrient transfer from nutrient-rich to nutrient-deprived areas facilitates bacterial OCP degradation in a nutrient-deficient habitat. The legacy pesticide hexachlorocyclohexane (HCH), a non-HCH-degrading fungus (Fusarium equiseti K3) and a co-metabolically HCH-degrading bacterium (Sphingomonas sp. S8) isolated from the same HCH-contaminated soil were used in spatially structured model ecosystems. Using 13C-labelled fungal biomass and protein-based stable isotope probing (protein-SIP), we traced the incorporation of 13C fungal metabolites into bacterial proteins while simultaneously determining the biotransformation of the HCH isomers. The relative isotope abundance (RIA, 7.1 - 14.2%), labeling ratio (LR, 0.13 - 0.35), and the shape of isotopic mass distribution profiles of bacterial peptides indicated the transfer of 13C-labeled fungal metabolites into bacterial proteins. Distinct 13C incorporation into the haloalkane dehalogenase (linB) and 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (LinC), as key enzymes in metabolic HCH degradation, underpin the role of mycelial nutrient transport and fungal-bacterial interactions for co-metabolic bacterial HCH degradation in heterogeneous habitats. Nutrient uptake from mycelia increased HCH removal by twofold as compared to bacterial monocultures. Fungal-bacterial interactions hence may play an important role in the co-metabolic biotransformation of OCP or recalcitrant micropollutants (MPs).

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Pesticide pestilence: Global scenario and recent advances in detection and degradation methods

Raj

Dubey

Malla

et al. 2023

Journal of Environmental Management

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Draft Genome Sequences of Two Sphingobium Species Associated with Hexachlorocyclohexane (HCH) Degradation Isolated from an HCH-Contaminated Soil

Cited by 4 publications

References 19 publications

Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

Pesticide pestilence: Global scenario and recent advances in detection and degradation methods

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