Draft Genome Sequences of Two Bacteria from the
            <i>Roseobacter</i>
            Group

Deogaygay, Xavier; Delherbe, Nathalie; Shikuma, Nicholas J.

doi:10.1128/mra.00390-21

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…We explored the compatibility of the toolkit with the tractable, phytoplankton-associated species of Phaeobacter gallaeciensis (87), and Ruegeria pomeroyi (88), and demonstrate transformation for the first time with invertebrate microbiome-associated strains Phaeobacter sp. HS012 (89) and Leisingera sp. 204H (90) (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

A modular plasmid toolkit applied in marine Proteobacteria reveals functional insights during bacteria-stimulated metamorphosis

Alker

Aspiras

Dunbar

et al. 2023

Preprint

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Section: Discussionmentioning

confidence: 99%

A modular plasmid toolkit applied in marine Proteobacteria reveals functional insights during bacteria-stimulated metamorphosis

Alker

Aspiras

Dunbar

et al. 2023

Preprint

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“…We tested the toolkit with the tractable, phytoplankton-associated species of Phaeobacter gallaeciensis ( 86 ), and Ruegeria pomeroyi ( 87 ), and demonstrated conjugation with invertebrate microbiome-associated strains Phaeobacter sp. HS012 ( 88 ) and Leisingera sp. 204H ( 89 ) ( Fig.…”

Section: Discussionmentioning

confidence: 99%

A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis

Alker,

Farrell,

Aspiras

et al. 2023

mBio

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A conspicuous roadblock to studying marine bacteria for fundamental research and biotechnology is a lack of modular synthetic biology tools for their genetic manipulation. Here, we applied, and generated new parts for, a modular plasmid toolkit to study marine bacteria in the context of symbioses and host-microbe interactions. To demonstrate the utility of this plasmid system, we genetically manipulated the marine bacterium Pseudoalteromonas luteoviolacea , which stimulates the metamorphosis of the model tubeworm, Hydroides elegans . Using these tools, we quantified constitutive and native promoter expression, developed reporter strains that enable the imaging of host-bacteria interactions, and used CRISPR interference (CRISPRi) to knock down a secondary metabolite and a host-associated gene. We demonstrate the broader utility of this modular system for testing the genetic tractability of marine bacteria that are known to be associated with diverse host-microbe symbioses. These efforts resulted in the successful conjugation of 12 marine strains from the Alphaproteobacteria and Gammaproteobacteria classes. Altogether, the present study demonstrates how synthetic biology strategies enable the investigation of marine microbes and marine host-microbe symbioses with potential implications for environmental restoration and biotechnology. IMPORTANCE Marine Proteobacteria are attractive targets for genetic engineering due to their ability to produce a diversity of bioactive metabolites and their involvement in host-microbe symbioses. Modular cloning toolkits have become a standard for engineering model microbes, such as Escherichia coli , because they enable innumerable mix-and-match DNA assembly and engineering options. However, such modular tools have not yet been applied to most marine bacterial species. In this work, we adapt a modular plasmid toolkit for use in a set of 12 marine bacteria from the Gammaproteobacteria and Alphaproteobacteria classes. We demonstrate the utility of this genetic toolkit by engineering a marine Pseudoalteromonas bacterium to study their association with its host animal Hydroides elegans . This work provides a proof of concept that modular genetic tools can be applied to diverse marine bacteria to address basic science questions and for biotechnology innovations.

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Draft Genome Sequences of Two Bacteria from the Roseobacter Group

Abstract: Here, we report the draft genome sequences of strains HS012 and HS039, which were isolated from cnidarian polyps that had recently undergone metamorphosis. Genomic analyses place these strains within the Phaeobacter and Leisingera genera, members of the Roseobacter group.

Cited by 2 publications

References 18 publications

A modular plasmid toolkit applied in marine Proteobacteria reveals functional insights during bacteria-stimulated metamorphosis

A modular plasmid toolkit applied in marine Proteobacteria reveals functional insights during bacteria-stimulated metamorphosis

A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis

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