Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky ST198 isolated from retail chicken carcasses in Egypt

Ramadan, Hazem; Gupta, Sushim K.; Sharma, Poonam; Sallam, Khalid Ibrahim; Hiott, Lari M.; Elsayed, Hagar; Barrett, John B.; Frye, Jonathan G.; Jackson, Charlene R.

doi:10.1016/j.jgar.2018.06.012

Cited by 15 publications

(11 citation statements)

References 6 publications

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“…Statistical analysis revealed that the mutations of gyrA (Ser83Phe, Asp87Gly) and parC (Ser80Ile) have a major influence on the high level of drug resistance of quinolones (ciprofloxacin, nalidixic acid, and ofloxacin). As can be seen in this study, quinolone resistance caused by the quinolone resistance-determination region mutation was the same as the internationally common ST198 resistance mechanism [26,27]. It is well known that quinolone-resistant ST198 is widespread in China, both in human and livestock sources.…”

Section: Discussionsupporting

confidence: 54%

“…It has been shown that S. Kentucky has been increasing its multidrug resistance rate since 2014. Fluoroquinolones are the preferred method of clinical treatment for nontyphoid salmonellosis [26]; at the same time, they are the main reasons for the spread of ST198 in the world [27]. The most important targets of fluoroquinolones for microorganisms are DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE); the mutations in the gyrA and parC genes are the most common mechanisms of bacterial resistance to quinolone antibiotics generally [28].…”

Section: Discussionmentioning

confidence: 99%

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Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

et al. 2020

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Salmonella enterica serovar Kentucky (S. Kentucky) sequence type 198 has emerged as a global zoonotic pathogen. We explored Salmonella enterica serovar Kentucky ST198 samples from the broiler chicken supply chain and patients between 2010 and 2016. Here, we collected 180 S. Kentucky isolates from clinical cases and the poultry supply chain. We performed XbaI pulsed-field gel electrophoresis and multilocus sequence typing. We assessed mutations in the quinolone resistance-determining regions and screened for the presence of the Salmonella genomic island 1 (SGI1). We determined that 63 (35.0%) of the 180 isolates were S. Kentucky ST198. Chinese strains of S. Kentucky ST198 have a high transmission of ciprofloxacin resistance (38/63, 60.3%) and a high risk of multidrug resistance. The quinolone resistance of the S. Kentucky ST198 strain found in China may be due to mutations in its quinolone resistance-determining region. Our study firstly revealed that ciprofloxacin-resistant S. Kentucky ST198 strains can undergo cross-host transmission, thereby causing a serious foodborne public health problem in China.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

et al. 2020

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“…All other patterns were observed among S. Kentucky isolates. Farms that are not displayed were negative for Salmonella (2,6,7,11,12,13,16,19). All farms that show a negative sign in the plasmid.…”

Section: Resultsmentioning

confidence: 99%

“…Ciprofloxacin-resistant S. Kentucky was attributed to illness in seven people and one death in the US after traveling from India [16]. In this regard, the emergence of S. Kentucky ST198, which is resistant to a number of critically important antibiotics, poses a major threat to public health worldwide since it is highly drug-resistant [18] and has been reported from different sources including retail chicken carcasses [19]. The presence of the mutation can be useful for tracking the pandemic ciprofloxacin-resistant S. Kentucky strain ST198 from geographically distinct regions [18].…”

Section: Discussionmentioning

confidence: 99%

International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda

et al. 2020

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The growing occurrence of multidrug-resistant (MDR) Salmonella enterica in poultry has been reported with public health concern worldwide. We reported, recently, the occurrence of Escherichia coli and Salmonella enterica serovars carrying clinically relevant resistance genes in dairy cattle farms in the Wakiso District, Uganda, highlighting an urgent need to monitor food-producing animal environments. Here, we present the prevalence, antimicrobial resistance, and sequence type of 51 Salmonella isolates recovered from 379 environmental samples from chicken farms in Uganda. Among the Salmonella isolates, 32/51 (62.7%) were resistant to at least one antimicrobial, and 10/51 (19.6%) displayed multiple drug resistance. Through PCR, five replicon plasmids were identified among chicken Salmonella isolates including IncFIIS 17/51 (33.3%), IncI1α 12/51 (23.5%), IncP 8/51 (15.7%), IncX1 8/51 (15.7%), and IncX2 1/51 (2.0%). In addition, we identified two additional replicons through WGS (Whole Genome Sequencing; ColpVC and IncFIB). A significant seasonal difference between chicken sampling periods was observed (p = 0.0017). We conclude that MDR Salmonella highlights the risks posed to animals and humans. Implementing a robust, integrated surveillance system will aid in monitoring MDR zoonotic threats.

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“…In this regard, the emergence of S . Kentucky ST198 pose a major threat to public health worldwide, particularly for being highly drug-resistant (15) and has been reported in different sources including retail chicken carcasses (16). Additionally, the presence of chromosome mutation can be useful for tracking the pandemic ciprofloxacin-resistant S. Kentucky strain ST198 from geographically distinct regions (15).…”

Section: Discussionmentioning

confidence: 99%

International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda

Ball¹,

Fedorka-Cray

Monte

et al. 2019

Preprint

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The growing occurrence of multidrug-resistant (MDR) Salmonella enterica in poultry has been reported with public health concern worldwide. We reported, recently, the occurrence of Escherichia coli and Salmonella enterica serovars carrying clinically relevant resistance genes in dairy cattle farms in the Wakiso District, Uganda, highlighting an urgent need to monitor food-producing animal environments. Here, we present the prevalence, antimicrobial resistance, and sequence type of 51 Salmonella isolates recovered from 400 environmental samples from chicken farms in Uganda. Among the Salmonella isolates, 32/51 (62.7%) were resistant to at least one antimicrobial, and 10/51 (19.6%) displayed multiple drug resistance. Through PCR, five replicon plasmids were identified among all chicken Salmonella including IncFIIS 17/51 (33.3%), IncI1α 12/51 (23.5%), IncP 8/51 (15.7%), IncX1 8/51 (15.7%), and IncX2 1/51 (2.0%). In addition, we identified replicons through WGS (ColpVC and IncFIB). A significant seasonal difference between chicken sampling periods was observed (p= 0.0017). We conclude that MDR Salmonella highlights the risks posed to the animals, environment, and humans for infection. Implementing a robust integrated surveillance system in Uganda will help monitor MDR to help control infectious threats.

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Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky ST198 isolated from retail chicken carcasses in Egypt

Cited by 15 publications

References 6 publications

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda

International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda

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