2008
DOI: 10.1309/d2m6q7byhwam1fyq
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DRAQ5-Based, No-Lyse, No-Wash Bone Marrow Aspirate Evaluation by Flow Cytometry

Abstract: Flow cytometry (FC) is a powerful tool for objective phenotyping of hematolymphoid neoplasia. Analysis of bone marrow aspirates and peripheral blood specimens by FC typically requires an erythrocyte lysis or gradient separation method to remove erythrocytes prior to analysis, which may result in the loss of certain populations, in particular nucleated erythroid cells. We developed a method to analyze bone marrow aspirates (BMAs) by FC without erythrocyte lysis or washing to minimize cell loss by exploiting the… Show more

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Cited by 16 publications
(18 citation statements)
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“…The use of a nuclear dye (such as Draq5 or Draq7) could be helpful in this respect. 14,15 For the morphological assessment of bone marrow smears of acute myeloid leukemia, it is common to …”
Section: Implementation Of Multiparameter Fc Analysis In Mdsmentioning
confidence: 99%
“…The use of a nuclear dye (such as Draq5 or Draq7) could be helpful in this respect. 14,15 For the morphological assessment of bone marrow smears of acute myeloid leukemia, it is common to …”
Section: Implementation Of Multiparameter Fc Analysis In Mdsmentioning
confidence: 99%
“…Yuan C et al. and others evaluated and showed the successful use of DRAQ5 along with surface staining in hematological samples (Smith et al., ; Yuan et al., ; Swerts et al., ; Allan et al., ). Nevertheless, these studies are limited to maximum three‐color immunophenotyping, which may not sufficiently separate a rare tumor population from its normal counterpart cells.…”
Section: Commentarymentioning
confidence: 99%
“…An alternative approach to study the erythroid compartment would be to use whole peripheral blood or BM with the addition of a nuclear dye in the MFC panel. To this avail, DRAQ5 or DRAQ7 (Biostatus Limited, Shepshed, UK) 12,13 for instance, allow to discriminate nucleated and non-nucleated cells in MFC.…”
Section: Introductionmentioning
confidence: 99%