Drebrin and Spermatogenesis

Chen, Haiqi; Li, Michelle W.M.; Cheng, C. Yan

doi:10.1007/978-4-431-56550-5_17

Cited by 4 publications

(2 citation statements)

References 120 publications

(170 reference statements)

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“…Considering the known actin-regulating activity of JMY, such we were prompted to determine if it interacts with these other actin cytoskeleton related proteins. Within this group, drebrin, actin-related protein 2/3 complex (Arp2/3), Anxa2, Cofilin, Gelsolin, spectrin have been reported to participate in regulating cell junction formation and function in Sertoli cells (Guttman et al, 2002; Lui et al, 2003; Lie et al, 2010; Li et al, 2011; Aristaeus de Asis et al, 2013; Chen et al, 2017; Chojnacka et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Functional importance of JMY expression by Sertoli cells in mediating mouse spermatogenesis

Fan

Yan

et al. 2018

Preprint

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3Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because 2 4 their actin cytoskeleton supports vesicle transport, cell junction, protein anchoring and 2 5 spermiation. Here, we show that junction-mediating and regulatory protein (JMY), an 2 6 actin regulating protein, also affects endocytic vesicle trafficking and Sertoli cell 2 7 junction remodeling since disruption of these functions induced male subfertility in 2 8Sertoli cell-specific Jmy knockout mice. Specifically, these mice have: a) impaired 2 9 BTB integrity and spermatid adhesion in the seminiferous tubules; b) high incidence 3 0 of sperm structural deformity; c) reduced sperm count and poor sperm motility. 3 1 Moreover, the cytoskeletal integrity in Sertoli cell-specific Jmy knockout mice was 3 2 compromised along with endocytic vesicular trafficking. These effects impaired 3 3 junctional protein recycling and reduced Sertoli cell junctions. In addition, JMY 3 4 interaction with α -actinin1 and Sorbs2 was related to JMY activity and in turn actin 3 5 cytoskeletal organization. In summary, JMY affects control of spermatogenesis 3 6 through regulating actin filament organization and endocytic vesicle trafficking in 3 7 Sertoli cells. 3 8 3 9

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Section: Resultsmentioning

confidence: 99%

Functional importance of JMY expression by Sertoli cells in mediating mouse spermatogenesis

Fan

Yan

et al. 2018

Preprint

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“…Considering the known actin-regulatory activity of JMY, this connection prompted us to determine whether it interacts with other actin cytoskeleton-related proteins. Within this group, Drebrin, Arp2/3, Anxa2, Cofilin, Gelsolin, and Spectrin have been reported to participate in regulating cell junction formation and function in Sertoli cells [30][31][32][33][34][35].…”

Section: Identification Of Proteins Interacting With Jmy By Immunopre...mentioning

confidence: 99%

JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice

et al. 2020

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Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because their actin cytoskeleton supports vesicular transport, cell junction formation, protein anchoring, and spermiation. Here, we show that a junction-mediating and actin-regulatory protein (JMY) affects the blood-tissue barrier (BTB) function through remodeling of the Sertoli cell junctional integrity and it also contributes to controlling endocytic vesicle trafficking. These functions are critical for the maintenance of sperm fertility since loss of Sertoli cell-specific Jmy function induced male subfertility in mice. Specifically, these mice have (a) impaired BTB integrity and spermatid adhesion in the seminiferous tubules; (b) high incidence of sperm structural deformity; and (c) reduced sperm count and poor sperm motility. Moreover, the cytoskeletal integrity was compromised along with endocytic vesicular trafficking. These effects impaired junctional protein recycling and reduced Sertoli cell BTB junctional integrity. In addition, JMY interaction with actin-binding protein candidates a-actinin1 and sorbin and SH3 domain containing protein 2 was related to JMY activity, and in turn, actin cytoskeletal organization. In summary, JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells.

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