DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells

Kawamori, Akihito; Yamaguchi, Masamitsu

doi:10.1247/csf.11004

Cited by 8 publications

(15 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent publication (44) has shown that DREF depletion in the notum and in other subdomains of the wing disc reduces or eliminates bristles. This phenotype is due in part to the influence of DREF on PCNA gene expression and affects the endoreplication process of bristle development, which results in a lack of bristles.…”

Section: Resultsmentioning

confidence: 99%

XNP/dATRX interacts with DREF in the chromatin to regulate gene expression

Valadéz-Graham

Yoshioka

Velazquez

et al. 2011

Nucleic Acids Research

Self Cite

View full text Add to dashboard Cite

The ATRX gene encodes a chromatin remodeling protein that has two important domains, a helicase/ATPase domain and a domain composed of two zinc fingers called the ADD domain. The ADD domain binds to histone tails and has been proposed to mediate their binding to chromatin. The putative ATRX homolog in Drosophila (XNP/dATRX) has a conserved helicase/ATPase domain but lacks the ADD domain. In this study, we propose that XNP/dATRX interacts with other proteins with chromatin-binding domains to recognize specific regions of chromatin to regulate gene expression. We report a novel functional interaction between XNP/dATRX and the cell proliferation factor DREF in the expression of pannier (pnr). DREF binds to DNA-replication elements (DRE) at the pnr promoter to modulate pnr expression. XNP/dATRX interacts with DREF, and the contact between the two factors occurs at the DRE sites, resulting in transcriptional repression of pnr. The occupancy of XNP/dATRX at the DRE, depends on DNA binding of DREF at this site. Interestingly, XNP/dATRX regulates some, but not all of the genes modulated by DREF, suggesting a promoter-specific role of XNP/dATRX in gene regulation. This work establishes that XNP/dATRX directly contacts the transcriptional activator DREF in the chromatin to regulate gene expression.

show abstract

Section: Resultsmentioning

confidence: 99%

XNP/dATRX interacts with DREF in the chromatin to regulate gene expression

Valadéz-Graham

Yoshioka

Velazquez

et al. 2011

Nucleic Acids Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…4) were obtained by dividing the number by the total number of the observations. Shaft and socket cells were identified by size, shape and location as previously described [30].…”

Section: Methodsmentioning

confidence: 99%

Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development

2012

Self Cite

View full text Add to dashboard Cite

Endoreplication is a variant type of DNA replication, consisting only of alternating G1 and S phases. Many types of Drosophila tissues undergo endoreplication. However, the timing and the extent to which a single endocycling macrochaete undergoes temporally programmed endoreplication during development are unclear. Here, we focused on the dynamics of endoreplication during posterior scutellar (pSC) macrochaete development. Quantitative analyses of C values in shaft cells and socket cells revealed a gradual rise from 8C and 4C at 8 hours after pupal formation (APF) to 72C and 24C at 29 hours APF, respectively. The validity of the values was further confirmed by the measurement of DNA content with a confocal laser microscope. BrdU incorporation assays demonstrated that shaft cells undergo four rounds of endoreplication from 18 to 29.5 hours APF. In contrast, socket cells undergo two rounds of endoreplication during the same period. Statistical analyses showed that the theoretical C values, based on BrdU assays, nearly coincide with the actually measured C values in socket cells, but not in shaft cells after 22 hours APF. These analyses suggest that socket cells undergo two rounds of endoreplication. However, the mechanism of endoreplication in the shaft cells may change from 22 hours APF, suggesting the possibility that shaft cells undergo two or four rounds of endoreplication during the periods. We also found that the timing of endoreplication differs, depending on the type of macrochaete. Moreover, endocycling in shaft cells of both the left and right sides of pSC bristle lineages occurs in the same pattern, indicating that the process is synchronized for specific types of macrochaete. Our findings suggest that endocycling in macrochaete cell lineages can be a model for understanding mechanisms of endoreplication at the single-cell level.

show abstract

“…To determine this, we carried out a quantitative genetic interaction analysis, as described earlier (Kawamori and Yamaguchi, 2011), using dref knockdown flies (UAS-DREF-RNAi 15 /1; sca-GAL4/1) and a loss of function allele, e2f1 rM729 . To determine this, we carried out a quantitative genetic interaction analysis, as described earlier (Kawamori and Yamaguchi, 2011), using dref knockdown flies (UAS-DREF-RNAi 15 /1; sca-GAL4/1) and a loss of function allele, e2f1 rM729 .…”

Section: Endoreplication Defects Caused By Dref Knockdown Are Partly mentioning

confidence: 99%

“…Then, why PCNA was activated with reduced levels of DREF? Length of PSC bristles were measured as detailed previously (Kawamori and Yamaguchi, 2011). In addition, E2F recognition sites in the PCNA promoter alone proved to be insufficient for promoter activity during embryonic and larval stages (Yamaguchi et al, 1995).…”

Section: Down-regulation Of Dref Stabilizes E2f1 Protein In Salivary mentioning

confidence: 99%

“…DREF, a BED finger type transcription regulatory factor, is required for efficient proliferation in mitotic cells (Hyun et al, 2005) and endoreplication (Hirose et al, 1999;Kawamori and Yamaguchi, 2011), forming homodimers, which bind to a DNA replication related element (DRE) (5 0 -TATCGATA) to positively regulate a wide variety of genes (Bruni et al, 2012;Emberly et al, 2008;Fernandez-Moreno et al, 2009;Fujiwara et al, 2012;Hirose et al, 1993Hirose et al, , 1996Killip and Grewal, 2012;Matsukage et al, 2008;Suyari et al, 2009;Trong-Tue et al, 2010;Valadez-Graham et al, 2012;Villanyi et al, 2008;Yoshioka et al, 2012). DREF is also associated with TRF2 and directs core-promoter recognition of the PCNA gene (Hochheimer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Control of e2f1 and PCNA by Drosophila transcription factor DREF

Kawamori

Shimaji

Yamaguchi

2013

Genesis

Self Cite

View full text Add to dashboard Cite

DREF (DNA replication-related element-binding factor), a zinc finger type transcription factor required for proper cell cycle progression in both mitotic and endocycling cells, is a positive regulator of E2F1, an important transcription factor which regulates genes related to the S-phase of the cell cycle. DREF and E2F1 regulate similar sets of replication-related genes, including proliferating cell nuclear antigen (PCNA), and play roles in the G1 to S phase transition. However, the relationships between dref and e2f1 or PCNA during development are poorly understood. Here, we provided evidence for novel control of e2f1 and PCNA involving DREF in endocycling cells. Somatic clone analysis demonstrated that dref knockdown stabilized E2F1 expression at posttranscriptional levels in endocycling salivary gland cells. Similarly, PCNA expression was up-regulated in the endocycling salivary gland cells. Genetic interaction analysis indicated that the endoreplication defects are partly caused via possible enhancement of E2F1 activity. From these results and previous reports, we conclude that regulation of e2f1 and PCNA by DREF in vivo is complex and the regulation mechanism may differ with the tissue and/or positions in the tissue.

show abstract

DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells

Cited by 8 publications

References 52 publications

XNP/dATRX interacts with DREF in the chromatin to regulate gene expression

XNP/dATRX interacts with DREF in the chromatin to regulate gene expression

Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development

Control of e2f1 and PCNA by Drosophila transcription factor DREF

Contact Info

Product

Resources

About