Dried Blood Spot Sample Collection, Storage, and Transportation

Mei, Joanne V.

doi:10.1002/9781118890837.ch3

Cited by 31 publications

(28 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To dry the blood spots, put the filter cards on a clean paper towel in a biohazard safety cabinet and let them dry, preferably O/N (but for at least 4 hrs), at RT in the absence of any external source of heat. When the drying process is complete, the blood spots have a uniformly dark brownish color and no red areas are visible anymore ( Figure 1D) 13,15,16 .…”

Section: Drying Of Blood Spotsmentioning

confidence: 99%

“…Since only small quantities of blood are used for DBS testing (one drop of capillary blood consists of approximately 50 µl) 5,16 , variations in the sample volume are crucial and, admittedly, at least some of the discrepancies between serological results and participant's self-reported HBV and HCV status recorded during the piloting of the "DRUCK Study" are attributable to this variable. The single most important factor for minimizing "fluctuations" of the sample volume is undoubtedly a correct technique of capillary blood collection, which can only be achieved by careful and ongoing training of the technical personnel 22 .…”

Section: Parametersmentioning

confidence: 99%

“…In the history of DBS testing many different filter paper cards have been used 2 but today only two commercial sources are approved by the FDA as class II medical devices for blood collection 5,16 . These filter card systems are highly uniform and have very similar absorption characteristics so that analytical results obtained from capillary blood prepared on either of them do not differ by more than 4% -5% 28 .…”

Section: Parametersmentioning

confidence: 99%

“…The single most important factor for minimizing "fluctuations" of the sample volume is undoubtedly a correct technique of capillary blood collection, which can only be achieved by careful and ongoing training of the technical personnel 22 . Furthermore, as a measure of quality control, all laboratories working with DBS should have already initiated procedures for identifying and subsequently excluding DBS specimens that have to be considered as unsatisfactory or invalid 16 .…”

Section: Parametersmentioning

confidence: 99%

“…For filter cards initially kept at ambient temperature, use a triple packaging system, which consists of the zipper bag(s) as the inner container(s) as well as an inner and an outer envelope. No content markings are required on the outer envelope for shipment by regular mail but the international biohazard symbol must be affixed to the primary inner container 16 . 4.…”

Section: Storage and Transportation Of Dried Blood Spots (Dbs)mentioning

confidence: 99%

See 4 more Smart Citations

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Grüner

Stambouli

Roß

2015

JoVE

125

110

View full text Add to dashboard Cite

The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resourcelimited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized. Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i.e., collection of blood; preparation of blood spots; drying of blood spots; storage and transportation of DBS; elution of DBS, and finally analyses of DBS eluates. The effectiveness of this protocol was first evaluated with 1,762 coupled serum/DBS pairs for detecting markers of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infections on an automated analytical platform. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.

show abstract

Section: Drying Of Blood Spotsmentioning

confidence: 99%

Section: Parametersmentioning

confidence: 99%

Section: Parametersmentioning

confidence: 99%

Section: Parametersmentioning

confidence: 99%

Section: Storage and Transportation Of Dried Blood Spots (Dbs)mentioning

confidence: 99%

See 3 more Smart Citations

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Grüner

Stambouli

Roß

2015

JoVE

125

110

View full text Add to dashboard Cite

show abstract

Dried blood spots: Effects of less than optimal collection, shipping time, heat, and humidity

Crimmins

Zhang²,

Kim

et al. 2020

American J Hum Biol

View full text Add to dashboard Cite

Objectives: This study investigates how factors related to collection, storage, transport time, and environmental conditions affect the quality and accuracy of analyses of dried blood spot (DBS) samples. Methods: Data come from the 2016 Health and Retirement Study (HRS) DBS laboratory reports and the HRS merged with the National Climatic Data Center (NCDC) Global Historical Climate Network Daily (NCDC GHCN-Daily) and the NCDC Local Climatological Data, by zip code. We ran regression models to examine the associations between assay values based on DBS for five analytes (total cholesterol, high-density lipoprotein (HDL) cholesterol, glycosylated hemoglobin (HbA1c), C-reactive protein (CRP), and cystatin C) and the characteristics of DBS cards and drops, shipping time, and temperature, and humidity at the time of collection. Results: We found cholesterol measures to be sensitive to many factors including small spots, shipping time, high temperature and humidity. Small spots in DBS cards are related to lower values across all analytes. Longer DBS transit time before freezing is associated with lower values of total and HDL cholesterol and cystatin C. Results were similar whether or not venous blood sample values were included in equations. Conclusions: Small spots, long shipping time, and exposure to high temperature and humidity need to be avoided if possible. Quality of spots and cards and information on shipping time and conditions should be coded with the data to make adjustments in values when necessary. The different results across analytes indicate that results cannot be generalized to all DBS assays.

show abstract

Dried blood spot collection, sample quality, and fieldwork conditions: Structural validations for conversion into standard values

Börsch‐Supan

Weiss

Börsch‐Supan³

et al. 2020

American J Hum Biol

View full text Add to dashboard Cite

Objectives: SHARE, a pan-European panel study in 27 European countries and Israel, has collected dried blood spot (DBS) samples from approximately 27 000 respondents in 13 countries. We aim to obtain factors to convert analyte values between DBS and venous blood samples (VBS) taking account of adverse fieldwork conditions such as small spot size, high temperature and humidity, short drying time and long shipment times.Methods: We obtained VBS and DBS from a set of 20 donors in a laboratory setting, and treated the DBS in a systematic and controlled fashion simulating SHARE fieldwork conditions. We used the 3420 outcomes to estimate from DBS analyte values the values that we would have obtained had it been feasible to collect and analyze the donors' venous blood samples.Results: The influence of field conditions and sample quality on DBS analyte values is significant and differs among assays. Varying spot size is the main challenge and affects all markers except HbA1c. Smaller spots lead to overly high measured levels. A missing desiccant is detrimental for all markers except CRP and tHb. The temperature to which the samples are exposed plays a significant role for HDL and CysC, while too brief a drying time affects CRP and CysC. Lab-based adjustment formulae only accounting for the differences between re-liquefied DBS and venous blood do not address these fieldwork conditions.Conclusions: By simulating adverse fieldwork conditions in the lab, we were able to validate DBS collected under such conditions and established conversion formulae with high prediction accuracy.

show abstract

Dried Blood Spot Sample Collection, Storage, and Transportation

Cited by 31 publications

References 19 publications

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried blood spots: Effects of less than optimal collection, shipping time, heat, and humidity

Dried blood spot collection, sample quality, and fieldwork conditions: Structural validations for conversion into standard values

Contact Info

Product

Resources

About