Dried Paper Discs for Serological Identification of Human Mycoplasmas

Dighero, Mabel W.; Bradstreet, C. M. Patricia; Andrews, B. E.

doi:10.1111/j.1365-2672.1970.tb02259.x

Cited by 17 publications

(6 citation statements)

References 5 publications

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“…As indicated by Dighero et al (1970) GIT is the simplest and most specific, but the least sensitive of the tests available. It depends on the direct inhibition of growth on solid medium by specific hyper-immune serum, and detects primarily surface antigens.…”

Section: Discussionmentioning

confidence: 98%

A study on Contagious Caprine Pleuropneumonia (CCPP) in goats at an export oriented abattoir, Debrezeit, Ethiopia

Eshetu

Yigezu

Asfaw

2007

Trop Anim Health Prod

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300 goat serum samples from an export-oriented abattoir were tested for contagious caprine pleuropneumonia antibodies by the complement fixation test. The disease prevalence was 31% with no significant differences (P > 0.05) between the regions "Borena", "Bale", "Afar" and "Jinka" or the age of the goats (P > 0.05). Gross pathology and histopathology of the lung primary lesions were indicative of pleuropneumonia caused by Mycoplasma capricolum subsp. capripneumoniae.

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Section: Discussionmentioning

confidence: 98%

A study on Contagious Caprine Pleuropneumonia (CCPP) in goats at an export oriented abattoir, Debrezeit, Ethiopia

Eshetu

Yigezu

Asfaw

2007

Trop Anim Health Prod

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“…As stated for other mycoplasmas (4,7,9), the inoculum size and the amount of antiserum were critical for the test, and standard conditions were absolutely required.…”

Section: Discussionmentioning

confidence: 99%

Modifications of the Growth Inhibition Test and its Application to Human T-Mycoplasmas

Black¹

1973

Applied Microbiology

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Several factors were found to influence the growth inhibition test. Inoculum size and amount of antiserum are well known variables, but the method of applying the antiserum, the incubation temperature, and the pH of the agar medium also play significant roles. The growth inhibition test modified according to these findings was found to be specific and well suited for the classification and identification of human T-mycoplasmas.

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“…Primary surface antigens of Mccp are also detected (Dighero et al. 1970). It is especially helpful for identifying the pathogen as they are serologically homogeneous and large inhibition zones are produced for various isolates.…”

Section: Diagnosismentioning

confidence: 99%

Contagious caprine pleuropneumonia – a comprehensive review

Yatoo

Parray

Bashir

et al. 2019

Veterinary Quarterly

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Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity ($100%), and mortality (80-100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.

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Dried Paper Discs for Serological Identification of Human Mycoplasmas

Cited by 17 publications

References 5 publications

A study on Contagious Caprine Pleuropneumonia (CCPP) in goats at an export oriented abattoir, Debrezeit, Ethiopia

A study on Contagious Caprine Pleuropneumonia (CCPP) in goats at an export oriented abattoir, Debrezeit, Ethiopia

Modifications of the Growth Inhibition Test and its Application to Human T-Mycoplasmas

Contagious caprine pleuropneumonia – a comprehensive review

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