Driving the new generation histone deacetylase inhibitors in cancer therapy; manipulation of the histone abbreviation at the epigenetic level: an in-silico approach

Abstract

Histone deacetylase (HDAC) is an enzyme that deacetylates the histone protein by removing the acetyl group from the lysine residues. The over-expression of the HDAC enzyme alters the gene expressions and causes cancer development in the human body. The inhibition of HDAC is an excellent therapeutic way in current cancer therapy. In this regard, various inhibitors were selected, and the inhibitory potential of these inhibitors was examined by molecular dynamics (MD) simulation followed by trajectory analysis and binding energy calculations. The selected clinical trial II and III phase inhibitors are TSA (trichostatin-A), TFMK (trifluoromethyl-ketone-9,9,9-trifluoro-8-oxo-N-phenylnonanamide), AKA (alpha-ketoamide-N-cyclohexyl-N-methyl-2-oxononanediamide), ITF2357 (givinostat), MS275 (entinostat), CI994 (tacedinaline), and SAHA (suberoylanilide hydroxamic acid). This computational study examines the atomic level description of the drug binding site on the HDLP enzyme and investigates the interaction of the HDAC inhibitors with the amino acid residues attached to the active site of the histone deacetylase-like protein (HDLP). Root-mean-square deviation, radius of gyration, hydrogen bond analysis, MM-PBSA, LIE, and semi-LIE calculations have revealed that the HDLP enzyme is more stabilized when bound to TSA, ITF2357, and reference inhibitor SAHA. It was observed that the hydroxamic acid family inhibitors have more potent in inhibiting the HDLP enzyme than the benzamide and ketone families. The inhibitory efficacy of TSA and ITF2357 is much similar to that of SAHA. Therefore, these HDAC inhibitors have the potential to be used in future clinical practices for cancer-related treatments. The knowledge gathered from this study could also lead to discovering new HDAC inhibitors for clinical research.

Exploration of Novel Mono Hydroxamic Acid Derivatives as Inhibitors for Histone Deacetylase Like Protein (HDLP) by Molecular Dynamics Studies

“…The acetylation of lysine residues found in the N terminal of core histone is one of the post-translational chromatin modifications that affect gene expression [6]. Acetylation and deacetylation of histones are restricted by the enzymes of histone acetyltransferase and histone deacetylase (HDACs) [7].…”