Driving to Safety: CRISPR-Based Genetic Approaches to Reducing Antibiotic Resistance

Bier, Ethan; Nizet, Victor

doi:10.1016/j.tig.2021.02.007

Cited by 9 publications

(6 citation statements)

References 139 publications

(184 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CRISPR-based gene editing has the potential to cause unintended mutations in the genome, which can lead to serious side effects. This is particularly concerning in cancer therapy, where the goal is to target specific genetic mutations that drive tumor growth [ 239 ]. One of the main safety concerns with CRISPR-based cancer therapy is the potential for non-targeted site effects.…”

Section: Safety and Delivery Challengesmentioning

confidence: 99%

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Chehelgerdi,

Khorramian-Ghahfarokhi

et al. 2024

Mol Cancer

View full text Add to dashboard Cite

The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has opened up new possibilities for the development of more effective and personalized cancer treatments. In this review, we will discuss the different CRISPR-based strategies that have been proposed for cancer therapy, including inactivating genes that drive tumor growth, enhancing the immune response to cancer cells, repairing genetic mutations that cause cancer, and delivering cancer-killing molecules directly to tumor cells. We will also summarize the current state of preclinical studies and clinical trials of CRISPR-based cancer therapy, highlighting the most promising results and the challenges that still need to be overcome. Safety and delivery are also important challenges for CRISPR-based cancer therapy to become a viable clinical option. We will discuss the challenges and limitations that need to be overcome, such as off-target effects, safety, and delivery to the tumor site. Finally, we will provide an overview of the current challenges and opportunities in the field of CRISPR-based cancer therapy and discuss future directions for research and development. The CRISPR system has the potential to change the landscape of cancer research, and this review aims to provide an overview of the current state of the field and the challenges that need to be overcome to realize this potential.

show abstract

Section: Safety and Delivery Challengesmentioning

confidence: 99%

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Chehelgerdi,

Khorramian-Ghahfarokhi

et al. 2024

Mol Cancer

View full text Add to dashboard Cite

show abstract

“…CRISPR-based drive-like systems, employing self-amplifying ‘pro-active genetic’ systems 52 or RNA-guided transposons 128 – 132 , have also been developed in bacteria, which, if combined with a means for horizontal gene transfer 133 – 141 , could help deplete antibiotic resistance factors from bacteria in the environment 142 , 143 and perhaps be adapted to fight bacterial infections 138 – 141 , 144 (reviewed in ref. 145 ).…”

Section: Developing Drive Systems In Other Organismsmentioning

confidence: 99%

Gene drives gaining speed

Bier

2021

Nat Rev Genet

Self Cite

148

View full text Add to dashboard Cite

Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian (>50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are highly efficient in laboratory settings, offering the potential to reduce the prevalence of vector-borne diseases, crop pests and non-native invasive species. However, concerns have been raised regarding the potential unintended impacts of gene-drive systems. This Review summarizes the phenomenal progress in this field, focusing on optimal design features for full-drive elements (drives with linked Cas9 and guide RNA components) that either suppress target mosquito populations or modify them to prevent pathogen transmission, allelic drives for updating genetic elements, mitigating strategies including trans -complementing split-drives and genetic neutralizing elements, and the adaptation of drive technology to other organisms. These scientific advances, combined with ethical and social considerations, will facilitate the transparent and responsible advancement of these technologies towards field implementation.

show abstract

“…This is because AMR targeting CRISPR Cas platforms are deployed and assessed using laboratory strains or from clinically isolated strains which may differ from the native strains that are hidden or encapsulated in the community. Consequently, target bacteria cannot be manipulated within a diverse population and hence robust conjugation systems of donors might prove to be less efficient in a complex environment where target bacteria may not be exposed easily [161].…”

Section: Complexity Of Microbial Communitiesmentioning

confidence: 99%

“…When considering the use of the CRISPR Cas system for targeting pathogens via horizontal gene transfer (conjugative plasmids), public opinion and acceptance of such systems will always be controversial as this involves the spread of transgene throughout native populations. Similarly, the use of the CRISPR Cas system in clinical settings needs to be thoroughly scrutinized and ought to face regulatory challenges due to its novelty [161].…”

Section: Regulatory Approvalmentioning

confidence: 99%

CRISPR-Cas System: A Tool to Eliminate Drug-Resistant Gram-Negative Bacteria

Kundar

Gokarn

2022

Pharmaceuticals

View full text Add to dashboard Cite

Rapidly emerging drug-resistant superbugs, especially Gram-negative bacteria, pose a serious threat to healthcare systems all over the globe. Newer strategies are being developed to detect and overcome the arsenal of weapons that these bacteria possess. The development of antibiotics is time-consuming and may not provide full proof of action on evolving drug-resistant pathogens. The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) systems are promising in curbing drug-resistant bacteria. This review focuses on the pathogenesis of Gram-negative bacteria, emergence of antimicrobial drug resistance, and their treatment failures. It also draws attention to the present status of the CRISPR-Cas system in diagnosisand treatment of Gram-negative bacterial infections.

show abstract

Driving to Safety: CRISPR-Based Genetic Approaches to Reducing Antibiotic Resistance

Cited by 9 publications

References 139 publications

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Gene drives gaining speed

CRISPR-Cas System: A Tool to Eliminate Drug-Resistant Gram-Negative Bacteria

Contact Info

Product

Resources

About