Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultra high dilutions

Kokornaczyk, Maria Olga; Trebbi, Grazia; Dinelli, Giovanni; Marotti, Ilaria; Bregola, Valeria; Nani, Daniele; Borghini, Francesco; Betti, Lucietta

doi:10.1016/j.ctim.2014.02.005

Cited by 24 publications

(26 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The influence of the factor day was significant in most of the here presented experiments (24/25 differentiation and 23/25 control experiments) (Tables 1-5). A significant influence of the experimentation day has been reported in many previous studies concerning methods based on phase-transition-induced pattern formation 11,13,14,[19][20][21][22] . This fact might be due to some day-to-day variations in the experiment performance or experimental conditions; or to other yet unknown and uncontrolled influences.…”

Section: Discussionmentioning

confidence: 99%

Impact of succussion on pharmaceutical preparations analyzed by means of patterns from evaporated droplets

Kokornaczyk

Würtenberger

Baumgartner

2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to investigate if patterns obtained from evaporating droplets of pharmaceutical preparations reveal the impact of succussion on such medicinal products. For this purpose, five pharmaceutical preparations (Echinacea 10−2, Baptisia 10−3, Baptisia 10−4, Luffa 10−4, and Spongia 10−6) were prepared according to the European Pharmacopoeia guidelines for the production of homeopathic remedies, in three variants each: with varying numbers of succussion strokes (i) 100, (ii) 10 (succussed samples), and (iii) zero (gently mixed, unsuccussed sample). System stability was studied by means of systematic positive control experiments. Patterns were evaluated by means of computerized image analysis regarding grey level distribution, texture, and fractality. For all investigated pharmaceutical preparations, significant differences were found between the succussed and gently mixed samples; whereas, all three samples (prepared with 100, 10 and zero succussion strokes) could be significantly differentiated for Luffa 10−4 and Spongia 10−6 for one image evaluation parameter each. Control experiments showed a reasonable stability of the experimental set-up.

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of succussion on pharmaceutical preparations analyzed by means of patterns from evaporated droplets

Kokornaczyk

Würtenberger

Baumgartner

2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some authors look for the differences in contrast, fractal dimension [8,24], while others find the symmetry of DRP images to be an important parameter for the measuring of differences using DEM [14,25]. A possible way to group the ways of pattern evaluation is also the one used in [26] which distinguishes between the sizing parameters and shape-descriptors.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, DEM has also been used to research the still controversial special characteristics of ultra-high diluted aqueous solutions [14]. It proved capable of demonstrating differences in subtle influences of a ultra-high dilution (around 10 −47 M, practically "pure" water) of As2O3 (UDH As) on common wheat seeds.…”

Section: Introductionmentioning

confidence: 99%

A Further Indication of the Self-Ordering Capacity of Water Via the Droplet Evaporation Method

Jerman

Ratajc

2014

Entropy

View full text Add to dashboard Cite

Abstract:The droplet evaporation method (DEM) is increasingly used for assessing various characteristics of water. In our research we tried to use DEM to detect a possible self-ordering capability of (spring) water that would be similar to the already found and described autothixotropic phenomenon, namely increasing order of non-distilled water subject to aging. The output of DEM is a droplet remnant pattern (DRP). For analysis of DRP images we used a specially developed computer program that does the frequency distribution analysis of certain parameters of the images. The results of experiments demonstrated statistically significant differences in both aging of water as well as in the glass exposed surface/volume ratio of the aged water. The most important result supporting the self-ordering character of water was found in an increasing dependence between two analyzed parameters: distance and frequency, at the peak frequency. As the result concerns mostly aging and shows increasing order it further corroborates other findings concerning increasing order by aging. Such further confirmation of self-ordering capacity of water is not important only for physical chemistry, but also for biology.

show abstract

“…1,2 Recent studies suggest that nanoparticles are formed in the process of some homeopathic drug potentiation. 3 Therefore, studying the physicochemical behaviour of active molecules used in homeopathic practice could contribute to reinforcing these issues with scientific bases. 4 Atropine is a molecule present in Belladonna atropa 5,6 ; however, in high concentrations, it can exert neurotoxic effects (e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Atropine in High Aqueous Dilutions for Homeopathic Use

et al. 2020

View full text Add to dashboard Cite

Background Atropine is a constitutive alkaloid of belladonna, which is widely used in homeopathic medicine in high dilutions (HDs). In HDs, atropine molecules are not correlated with the energy of electrophysical interactions or the presence of aggregates, resulting in neurotoxic effects. However, in small concentrations at the nanoscale, its effects could present therapeutic advantages. Objective In the present study, the physicochemical properties of a range of HDs of atropine were analysed to establish a correlation between its electrical and morphological properties. Methods The electrodynamic properties were analysed with a conductivity meter, whereas the size and morphology of the nanoparticles were evidenced by transmission electron microscopy and atomic force microscopy. The HDs were prepared with deionised water under sterile conditions. Hydrogen potential (pH) and electrical potential were determined with a portable equipment. Electrical conductivity (EC) was calculated using the ionic strength and Debye length. Results The characterisation of atropine HDs showed the formation of aggregates and the fluctuation of morphological variables at each dilution. Electromagnetic and pH changes were evidenced, which could contribute to a change in the EC. Possibly being responsible for a better response at the cellular level without triggering side effects in the individual.These data suggest that the forces of Derjaguin–Landau–Verwey–Overbeek and the forces of Van der Waals are involved in the variety of forms among these aggregates.The EC was significant at 200°C. This variation suggests changes in the electromagnetic configuration of the HDs. It is only through the conformation of nanoparticle aggregates that these variations can be triggered. Conclusion Atropine HDs have demonstrated the presence of nanoparticle aggregates with different morphological and electromagnetic properties. Our results may be relevant for future approaches focused on the use of atropine in HDs to minimise cytotoxic effects or modulate adverse effects in patients with neuropathy.Until now, the therapeutic effects of homeopathy have not been relevant to the scientific community; however, here we demonstrate with scientific approaches the conformation of aggregates of nanoparticles present in HDs that modify their structure and electromagnetism, properties that could produce differential biological responses without generating cytotoxicity.

show abstract

Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultra high dilutions

Cited by 24 publications

References 46 publications

Impact of succussion on pharmaceutical preparations analyzed by means of patterns from evaporated droplets

Impact of succussion on pharmaceutical preparations analyzed by means of patterns from evaporated droplets

A Further Indication of the Self-Ordering Capacity of Water Via the Droplet Evaporation Method

Characterisation of Atropine in High Aqueous Dilutions for Homeopathic Use

Contact Info

Product

Resources

About