Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath

Liu, Tie‐Yuan; Ye, Nenghui; Wang, Xinyu; Das, Debatosh; Tan, Yuxiang; You, Xiangkai; Long, M. I. E.; Hu, Tianming; Dai, Lei; Zhang, Jianhua; Chen, Moxian

doi:10.1111/jipb.13154

Cited by 44 publications

(18 citation statements)

References 97 publications

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“…Information about accession and ecotypic differences in the saponin content could provide tools for improvement of switchgrass biomass. For example, avenacins have well documented protective roles protecting oat roots from the fungal “take-all” disease. − Saponin root differential accumulation among switchgrass ecotypes suggests that they might be attractive targets for breeding cultivars with an increased ability to improve yield by modulating the microbiome structure and function. − In contrast, breeding for low leaf saponins might produce varieties with biomass that is efficiently converted into fuel by avoiding accumulation of toxins that interfere with growth of processing microbes. Taken together, our results provide opportunities to identify targets for producing switchgrass varieties with improved plant/microbiome traits and increased biomass yield or biofuel conversion at lower economic and environmental costs.…”

Section: Discussionmentioning

confidence: 99%

Switchgrass Metabolomics Reveals Striking Genotypic and Developmental Differences in Specialized Metabolic Phenotypes

Sarma

Sumner

et al. 2022

J. Agric. Food Chem.

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Switchgrass (Panicum virgatum L.) is a bioenergy crop that grows productively on lands not suitable for food production and is an excellent target for low-pesticide input biomass production. We hypothesize that resistance to insect pests and microbial pathogens is influenced by low-molecular-weight compounds known as specialized metabolites. We employed untargeted liquid chromatography–mass spectrometry, quantitative gas chromatography–mass spectrometry (GC–MS), and nuclear magnetic resonance spectroscopy to identify differences in switchgrass ecotype metabolomes. This analysis revealed striking differences between upland and lowland switchgrass metabolomes as well as distinct developmental profiles. Terpenoid- and polyphenol-derived specialized metabolites were identified, including steroidal saponins, di- and sesqui-terpenoids, and flavonoids. The saponins are particularly abundant in switchgrass extracts and have diverse aglycone cores and sugar moieties. We report seven structurally distinct steroidal saponin classes with unique steroidal cores and glycosylated at one or two positions. Quantitative GC–MS revealed differences in total saponin concentrations in the leaf blade, leaf sheath, stem, rhizome, and root (2.3 ± 0.10, 0.5 ± 0.01, 2.5 ± 0.5, 3.0 ± 0.7, and 0.3 ± 0.01 μg/mg of dw, respectively). The quantitative data also demonstrated that saponin concentrations are higher in roots of lowland (ranging from 3.0 to 6.6 μg/mg of dw) than in upland (from 0.9 to 1.9 μg/mg of dw) ecotype plants, suggesting ecotypic-specific biosynthesis and/or biological functions. These results enable future testing of these specialized metabolites on biotic and abiotic stress tolerance and can provide information on the development of low-input bioenergy crops.

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Section: Discussionmentioning

confidence: 99%

Switchgrass Metabolomics Reveals Striking Genotypic and Developmental Differences in Specialized Metabolic Phenotypes

Sarma

Sumner

et al. 2022

J. Agric. Food Chem.

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“…However, the diversity of bacteria first decreased and then increased, which suggested that some drought-intolerant marine bacteria decreased during water loss. For example, both Cyanobacteria and Gemmatimonadetes have been reported to be sensitive to drought, and their abundances decreased after water loss in previous studies ( 34 , 35 ). But, with the increase in water loss duration, the number of some drought-resistant bacteria increased, which led to an increase in diversity.…”

Section: Discussionmentioning

confidence: 83%

Effects of Water Loss Stress under Tidal Effects on the Epiphytic Bacterial Community of Sargassum thunbergii in the Intertidal Zone

Sun

Yang

et al. 2022

mSphere

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Periodic water loss caused by the tide is an important environmental factor that is faced by intertidal macroalgae, but the impact of periodic water loss on the epiphytic bacterial communities associated with macroalgae is still unknown. Through this study, we found that the diversity, the relative abundance of dominant taxa, the indicator species, and the abundance of the predicted functional genes in the epiphytic bacteria on S. thunbergii changed with the time of water loss.

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“…Also, recent studies have demonstrated bioactive triterpenoids in roots exudates of soybean ( Glycine max ) and tomato ( Solanum lycopersicum ) that aid the assembly of the root microbiome to confer robustness against environmental stresses (Fujimatsu et al 2020; Nakayasu et al 2021). While distinct microbiome responses to drought stress have been reported in upland and lowland switchgrass ecotypes (Liu et al 2021), the role of specialized metabolites such as saponins in these interactions is yet to be discovered.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics and metabolomics reveal specialized metabolite drought stress responses in switchgrass (Panicum virgatumL.)

Tiedge

Merrill

et al. 2022

Preprint

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SUMMARYSwitchgrass (Panicum virgatum) is a bioenergy model crop valued for its energy efficiency and drought tolerance resilience. The related monocot species rice (Oryza sativa) and maize (Zea mays) deploy species-specific, specialized metabolites as core stress defenses. By contrast, specialized chemical defenses in switchgrass are largely unknown.To investigate specialized metabolic drought responses in switchgrass, we integrated tissue-specific transcriptome and metabolite analyses of the genotypes Alamo and Cave-in-Rock that feature different drought tolerance.The more drought-susceptible Cave-in-Rock featured an earlier onset of transcriptomic changes and significantly more differentially expressed genes in response to drought compared to Alamo. Specialized pathways showed moderate differential expression compared to pronounced transcriptomic alterations in carbohydrate and amino acid metabolism. However, diterpenoid-biosynthetic genes showed drought-inducible expression in Alamo roots, contrasting largely unaltered triterpenoid and phenylpropanoid pathways. Metabolomic analyses identified common and genotype-specific flavonoids and terpenoids. Consistent with transcriptomic alterations, several root diterpenoids showed significant drought-induced accumulation, whereas triterpenoid abundance remained predominantly unchanged. Structural analysis of drought-responsive root diterpenoids verified these metabolites as oxygenated furanoditerpenoids.Drought-dependent transcriptome and metabolite profiles provide the foundation to understand the molecular mechanisms underlying switchgrass environmental resilience. Accumulation of specialized root diterpenoids and corresponding pathway transcripts supports a role in drought stress tolerance for these compounds.Significance statementWith an increasing demand for renewable energy opposed by rising climate-driven crop losses, understanding, and leveraging plant natural defenses can enable the development of sustainable crop production systems. Here, we integrated comparative transcriptomics and metabolomics analyses to gain a detailed understanding of the diversity and physiological relevance of specialized metabolites in upland and lowland switchgrass ecotypes and provide resources for future investigations of drought response mechanisms in switchgrass.

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Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath

Cited by 44 publications

References 97 publications

Switchgrass Metabolomics Reveals Striking Genotypic and Developmental Differences in Specialized Metabolic Phenotypes

Switchgrass Metabolomics Reveals Striking Genotypic and Developmental Differences in Specialized Metabolic Phenotypes

Effects of Water Loss Stress under Tidal Effects on the Epiphytic Bacterial Community of Sargassum thunbergii in the Intertidal Zone

Comparative transcriptomics and metabolomics reveal specialized metabolite drought stress responses in switchgrass (Panicum virgatumL.)

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