Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor

Wang, Nan; Zhang, Wenxiao; Qin, Mengyin; Li, Shuo; Qiao, Meng; Liu, Zhenhua; Xiang, Fengning

doi:10.1093/pcp/pcx111

Cited by 117 publications

(77 citation statements)

References 58 publications

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“…RnA isolation and cDnA synthesis. For RNA-Sequencing (RNA-Seq) and qRT-PCR analysis, total RNA was extracted from 250 mg frozen root tissue using a phenol-chloroform extraction method 77 . RNA concentration was quantified photometrically using a NanoDrop (ND-1000, Thermo Scientific, Wilmington, DE, USA) and RNA integrity was tested on 1.2% agarose gel.…”

Section: Methodsmentioning

confidence: 99%

RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato root

Safavi-Rizi

Herde

Stöhr

2020

Sci Rep

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Due to climate change, economically important crop plants will encounter flooding periods causing hypoxic stress more frequently. this may lead to reduced yields and endanger food security. As roots are the first organ to be affected by hypoxia, the ability to sense and respond to hypoxic stress is crucial. At the molecular level, therefore, fine-tuning the regulation of gene expression in the root is essential for hypoxia tolerance. Using an RnA-Seq approach, we investigated transcriptome modulation in tomato roots of the cultivar 'Moneymaker', in response to short-(6 h) and long-term (48 h) hypoxia. Hypoxia duration appeared to have a significant impact on gene expression such that the roots of five weeks old tomato plants showed a distinct time-dependent transcriptome response. We observed expression changes in 267 and 1421 genes under short-and long-term hypoxia, respectively. Among these, 243 genes experienced changed expression at both time points. We identified tomato genes with a potential role in aerenchyma formation which facilitates oxygen transport and may act as an escape mechanism enabling hypoxia tolerance. Moreover, we identified differentially regulated genes related to carbon and amino acid metabolism and redox homeostasis. Of particular interest were the differentially regulated transcription factors, which act as master regulators of downstream target genes involved in responses to short and/or long-term hypoxia. Our data suggest a temporal metabolic and anatomic adjustment to hypoxia in tomato root which requires further investigation. We propose that the regulated genes identified in this study are good candidates for further studies regarding hypoxia tolerance in tomato or other crops.

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Section: Methodsmentioning

confidence: 99%

RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato root

Safavi-Rizi

Herde

Stöhr

2020

Sci Rep

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“…Suc non-fermenting-1-related protein kinase2 is implicated in stress signaling transduction via abscisic acid-dependent and -independent pathways (Yoshida et al, 2002;Zhang et al, 2011), TaBZR2 could modulate the expression of numerous stress-responsive genes under drought conditions, contributing to the drought tolerance of the transgenic wheat. ), which, if not controlled, can eventually lead to oxidative damage (Dat et al, 2000;Wang et al, 2017). TaBZR2 has a role in activating antioxidant enzyme gene expression.…”

Section: Tabzr2 Positively Regulates the Expression Of Multiple Stresmentioning

confidence: 99%

BES/BZR Transcription Factor TaBZR2 Positively Regulates Drought Responses by Activation of TaGST1

et al. 2019

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BRI1-EMS suppressor (BES)/brassinazole-resistant (BZR) family transcription factors are involved in a variety of physiological processes, but the biological functions of some BES/BZR transcription factors remain unknown; moreover, it is not clear if any of these proteins function in the regulation of plant stress responses. Here, wheat (Triticum aestivum) brassinazole-resistant 2 (TaBZR2)-overexpressing plants exhibited drought tolerant phenotypes, whereas downregulation of TaBZR2 in wheat by RNA interference resulted in elevated drought sensitivity. electrophoretic mobility shift assay and luciferase reporter analysis illustrate that TaBZR2 directly interacts with the gene promoter to activate the expression of T. aestivum glutathione s-transferase-1 (TaGST1), which functions positively in scavenging drought-induced superoxide anions (O 2 2). Moreover, TaBZR2 acts as a positive regulator in brassinosteroid (BR) signaling. Exogenous BR treatment enhanced TaBZR2-mediated O 2 2 scavenging and antioxidant enzyme gene expression. Taken together, we demonstrate that a BES/BZR family transcription factor, TaBZR2, functions positively in drought responses by activating TaGST1 and mediates the crosstalk between BR and drought signaling pathways. Our results thus provide new insights into the mechanisms underlying how BES/BZR family transcription factors contribute to drought tolerance in wheat.

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“…H 2 O 2 -correlated hub genes differentially regulated in response to drought stress Transcription factors are key players in the regulatory networks for plant responding to unfavorable stresses. The MYB family is one of the largest transcription factor families in plants with a conserved MYB binding domain and it has been showed to be essential for plants in response to abiotic stresses (Li et al 2015), as well as, to be induced by H 2 O 2 in soybean (Wang et al 2017). In this study, ve differentially expressed genes that are annotated as transcription factors, including 3 MYB, 1 NAD, and 1 SHR families, were highly clustered in Grey60, a module that was statistically correlated with the accumulation of H 2 O 2 during drought stress.…”

Section: Discussionmentioning

confidence: 99%

Identification of key modules and hub genes regulating drought stress response in rice drought sensitive line PY6 by weighted gene co-expression network analysis

Yu¹,

Liu²,

Wu³

et al. 2020

Preprint

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Background: Drought stress is an adverse factor with deleterious effects on several facets of rice growth. However, the mechanism underlying drought resistance in rice remains unclear. In order to genetically understand the potential molecular mechanism for drought response in rice, a drought sensitive Chromosome Segment Substitution Line (CSSL) PY6, which was constructed by the introgression of genomic segments of drought sensitive variety LAMBAYEQUE1 into drought-resistance variety PR403 via backcrossing, was used to map the QTL locus dss-1 for its sensitive phenotype, and to reveal the impact of dss-1 on the transcriptional profiling of PY6 via RNA-seq and WGCNA (weighted gene co-expression network analysis) analysis. Results: The genetic linkage analysis showed that dss-1 was located on the short arm of chromosome 1 of rice. In contrast to PR403, the over-accumulation of H 2 O 2 and MDA that might result in drought sensitive phenotype was observed in PY6 under drought stress. In the analysis of RNA-seq data, the identified differentially expressed genes (DEGs) mainly enriched in photosynthesis-related GO terms and exhibited a down-regulation pattern of their expressions in both PY6 and PR403 in response to drought stress, indicating that the photosynthesis was greatly inhibited in rice. Further WGCNA analysis constructed a co-expression network with 26 gene modules in which 4 and 3 modules that were highly correlated with H 2 O 2 and MDA, respectively. Likewise, the GO analysis of the differentially expressed hub genes (DEHGs) enriched in H 2 O 2 -correlated modules showed that the photosynthesis related GO terms were consistently over-represented. Furthermore, functional annotation of DEHGs in H 2 O 2 and MDA correlated modules revealed a cross talk between abiotic and biotic stresses. This was reflected by the differential expression alterations of hub genes which were annotated as encoding MYBs, laccases, WRKYs, and PRs family proteins, and ZFP36 were notably observed between PY6 and PR403 in response drought stress. Conclusions: Collectively, we speculated that drought-induced the inhibition of photosynthesis lead to the accumulation of H 2 O 2 and MDA that can trigger the reprogramming the profiling of transcriptome in rice. This included the differential regulation of hub genes that involve in ROS eliminated pathways to prevent the damage of rice plants from oxidative stress.

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Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor

Cited by 117 publications

References 58 publications

RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato root

RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato root

BES/BZR Transcription Factor TaBZR2 Positively Regulates Drought Responses by Activation of TaGST1

Identification of key modules and hub genes regulating drought stress response in rice drought sensitive line PY6 by weighted gene co-expression network analysis

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