Drought tolerance in tobacco

Holmström, Kjell‐Ove; Mäntylä, Einar; Welin, Björn; Mandal, Abul; Palva, E. Tapio; Tunnela, Outi E.; Londesborough, John

doi:10.1038/379683a0

Cited by 290 publications

(158 citation statements)

References 7 publications

Supporting

Mentioning

145

Contrasting

Unclassified

Order By: Relevance

“…As previously reported (Holmström et al 1996;Goddijn et al 1997;Romero et al 1997;Yeo et al 2000;Cortina and Culiáñez-Macià 2005), plants transformed with TPS1 alone showed slow growth, sterility and dark-green leaves. On the other hand, the expression of yeast TPS1-TPS2 in Arabidopsis did not provoke growth or morphological alterations, even if the strong and constitutive 35S promoter was used.…”

Section: Discussionsupporting

confidence: 78%

“…Heterologous expression of the E. coli or yeast TPS1 gene in plants leads to pronounced abnormalities in growth, development and metabolism (Holmström et al 1996;Goddijn et al 1997;Romero et al 1997;Yeo et al 2000;Cortina and Culiáñez-Macià 2005). Overexpression of the Arabidopsis AtTPS1 gene in its homologous background does not produce such drastic eVects but still there is a delay in Xowering (Avonce et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…For example, the overexpression of the TPS1 gene in tobacco, potato and tomato improved drought tolerance (Holmström et al 1996;Romero et al 1997;Yeo et al 2000;Cortina and Culiáñez-Macià 2005). Transgenic rice expressing the E. coli TPS-TPP were tolerant to drought, chilling and salt stress (Garg et al 2002;Jang et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Transgenic TPS1-TPS2 lines have normal growth and morphology Several reports have documented that overexpression of TPS genes of diVerent origin in plants provoke growth and morphological abnormalities with variable severity (Holmström et al 1996;Goddijn et al 1997;Romero et al 1997;Yeo et al 2000;Avonce et al 2004;Cortina and Culiáñez-Macià 2005). Therefore, we analyzed possible growth and shape abnormalities in transgenic plants overexpressing yeast TPS-TPP enzyme.…”

Section: Arabidopsis Tps1-tps2 Lines Accumulate Trehalose and Are Glumentioning

confidence: 98%

See 3 more Smart Citations

A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis

Miranda

Avonce

Suárez

et al. 2007

Planta

182

View full text Add to dashboard Cite

Improving stress tolerance is a major goal for agriculture. Trehalose is a key molecule involved in drought tolerance in anhydrobiotic organisms. Here we describe the construction of a chimeric translational fusion of yeast trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. This construct was overexpressed in yeast cells displaying both TPS and TPP enzyme activities and trehalose biosynthesis capacity. In Arabidopsis thaliana, the gene fusion was overexpressed using either the 35S promoter or the stress-regulated rd29A promoter. Transgene insertion in the genome was checked by PCR and transcript expression by RT-PCR. Several independent homozygous lines were selected in the presence of kanamycin and further analyzed. Trehalose was accumulated in all these lines at low levels. No morphological or growth alterations were observed in lines overexpressing the TPS1-TPS2 construct, whereas plants overexpressing the TPS1 alone under the control of the 35S promoter had aberrant growth, color and shape. TPS1-TPS2 overexpressor lines were glucose insensitive, consistent with a suggested role of trehalose/T6P in modulating sugar sensing and carbohydrate metabolism. Moreover, TPS1-TPS2 lines displayed a signiWcant increase in drought, freezing, salt and heat tolerance. This is the Wrst time that trehalose accumulation in plants is shown to protect against freezing and heat stress. Therefore, these results demonstrate that engineering trehalose metabolism with a yeast TPS-TPP bifunctional enzyme confers multiple stress protection in plants, comprising a potential tool to improve stress-tolerance in crops.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Arabidopsis Tps1-tps2 Lines Accumulate Trehalose and Are Glumentioning

confidence: 98%

See 2 more Smart Citations

A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis

Miranda

Avonce

Suárez

et al. 2007

Planta

182

View full text Add to dashboard Cite

show abstract

“…Several observations indicate that Tps1 may carry the catalytic activity of the Tre6P synthase: (i) tps1⌬ strains lack any detectable Tre6P synthase activity (Bell et al, 1992); (ii) TPS1 expression in Escherichia coli and in yeast (W. Bell et al, submitted) results in greatly increased Tre6P synthase activity; (iii) TPS1 complements the trehalose-synthesis defect of an E. coli otsA mutant (McDougall et al, 1993); and (iv) transgenic tobacco plants expressing TPS1 are able to synthesize trehalose (Holmströ m et al, 1996). It was therefore surprising that TPS1 was found to be identical to the previously sequenced CIF1 (catabolite inactivation of fructose-1,6-bisphosphatase), a gene reported to be essential for growth on glucose in Saccharomyces cerevisiae (Gonzá lez et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Structural analysis of the subunits of the trehalose‐6‐phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock

Reinders

Bürckert

Hohmann

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

SummarySynthesis of trehalose in the yeast Saccharomyces cerevisiae is catalysed by the trehalose-6-phosphate (Tre6P) synthase/phosphatase complex, which is composed of at least three different subunits encoded by the genes TPS1, TPS2, and TSL1. Previous studies indicated that Tps1 and Tps2 carry the catalytic activities of trehalose synthesis, namely Tre6P synthase (Tps1) and Tre6P phosphatase (Tps2), while Tsl1 was suggested to have regulatory functions. In this study two different approaches have been used to clarify the molecular composition of the trehalose synthase complex as well as the functional role of its potential subunits. Two-hybrid analyses of the in vivo interactions of Tps1, Tps2, Tsl1, and Tps3, a protein with high homology to Tsl1, revealed that both Tsl1 and Tps3 can interact with Tps1 and Tps2; the latter two proteins also interact with each other. In addition, trehalose metabolism upon heat shock was analysed in a set of 16 isogenic yeast strains carrying deletions of TPS1, TPS2, TSL1, and TPS3 in all possible combinations. These results not only confirm the previously suggested roles for Tps1 and Tps2, but also provide, for the first time, evidence that Tsl1 and Tps3 may share a common function with respect to regulation and/or structural stabilization of the Tre6P synthase/ phosphatase complex in exponentially growing, heatshocked cells.

show abstract

Two-dimensional electrophoresis of soluble leaf proteins, isolated from two wheat species (Triticum durum andTriticum aestivum) differing in sensitivity towards NaCl

Ouerghi¹,

Rémy

Ouelhazi

et al. 2000

Electrophoresis

View full text Add to dashboard Cite

Plants of two wheat species (Triticum aestivum cv. Tanit and T. durum cv. Ben Bachir), differing in their sensitivity to NaCl were cultivated in the presence or absence of 100 mM NaCl for 21 days. Soluble proteins extracted from leaves were analyzed by two-dimensional electrophoresis in order to detect NaCl-induced changes in the polypeptide patterns. In all, 500 spots were detected. Results showed species-dependent differences. The greatest alterations in the polypeptide profiles following salt stress were found in the most sensitive cultivar: among the 12 spots (molecular mass, 15-31 kDa) specifically considered in the acidic region of the gel, 11 declined, even disappeared in the NaCl-sensitive leaf profiles, while in the tolerant species only five spots were affected by the salt treatment and five remained untouched; moreover in the latter, two new polypeptides were shown to be induced by NaCl.

show abstract

Drought tolerance in tobacco

Cited by 290 publications

References 7 publications

A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis

A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis

Structural analysis of the subunits of the trehalose‐6‐phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock

Two-dimensional electrophoresis of soluble leaf proteins, isolated from two wheat species (Triticum durum andTriticum aestivum) differing in sensitivity towards NaCl

Contact Info

Product

Resources

About