Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco

Feng, Liang; Xing, Shichao; Guo, Qifang; Zhao, Meirong; Zhang, Jin; Gao, Qiang; Wang, Guiping; Wang, Wei

doi:10.1016/j.jplph.2010.11.023

Cited by 101 publications

(60 citation statements)

References 35 publications

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“…These transgenic plants are capable of increasing cell division and developing roots longer than in the control plants. Li et al (2011) examined the drought resistance of transgenic tobaccos overexpressing TaEXPB23, which were cloned from wheat. The results indicated that the transgenic tobacco lines lost water more slowly than the wild-type plants under drought stress; their cells could sustain a more integrated structure under water stress than that of the wild type.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

Lo¹,

Le²,

Nguyen³

et al. 2015

Turk J Bot

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The EXP1 gene encodes expansin, which has the ability to loosen the plant cell wall. The soybean expansin gene GmEXP1 is activated specifically during the root elongation process, and thus it plays important roles in root development. During the drought period, changes in pressure within the cell and the fast development of the root allow plants to collect water from deep soil, which in turn helps plants grow and develop. In this study, we have successfully cloned and generated a GmEXP1 construct expressing recombinant expansin protein in tobacco plants. GmEXP1 is expressed in transgenic tobacco plants and passed on to the next generation. The transgenic tobacco plants have improved drought tolerance, which is demonstrated in both the length and volume of roots. From these promising results, we applied the same approach to generate drought-tolerant plants.

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Section: Discussionmentioning

confidence: 99%

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

Lo¹,

Le²,

Nguyen³

et al. 2015

Turk J Bot

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“…After 2 days of cultivation, the concentration of nutrients was increased to half-strength and, after 4 days of cultivation, to full strength. The nutrient solution had the following composition: 2.5 mM Ca(NO 3 24 , 200 M Fe-EDTA. The solution was changed every second day to avoid nutrient depletion.…”

Section: Methodsmentioning

confidence: 99%

“…For example, they have been identified as being involved in the salinity stress response in wheat (18), maize (19), Arabidopsis (20), roses (21), and sorghum (22) or under conditions of water deficit (23)(24)(25)(26)(27) or water excess and anoxia (28 -31). Recently, genotypic differences in ␤-expansin abundance have been demonstrated to occur between salt-stressed leaves of two maize cultivars that differ in their ability to grow under saline conditions (19,32).…”

mentioning

confidence: 99%

Down-Regulation of ZmEXPB6 (Zea mays β-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves of Z. mays L.

Geilfus

Ober²,

Eichacker

et al. 2015

Journal of Biological Chemistry

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Background: Maize shows a rapid leaf growth reduction upon salinity, being a thread to global food security. Results: Exogenous application of Zea mays ␤-expansin 6 partially restores growth. Conclusion: ZmEXPB6 improves growth by acting on the capacities of cell walls to extend. Significance: A strategy to improve growth under saline conditions was shown. This knowledge can be useful for developing crops with a better performance under salinity.

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“…Li et al (2011) produced transgenic tobacco plants with increased tolerance to water stress by overexpressing the wheat expansin gene TaEXPB23 driven by the constitutive 35S cauliflower mosaic virus (CaMV) promoter. However, the growth and development of 35S::TaEXPB23 transgenic tobacco plants were altered under normal growth conditions, with a faster growth rate at the seedling stage, earlier flowering and maturation, and a shorter plant height compared to WT plants.…”

Section: Water Stress Tolerancesmentioning

confidence: 99%

Current trends in genetic manipulations to enhance abiotic and biotic stresses in tobacco

Tesfaw¹,

Feyissa²

2014

Afr. J. Biotechnol.

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Environmental stresses, both abiotic and biotic, are the main factors reducing crops productivity. Biotic stresses caused by fungi, viruses, bacteria and insects are the major threats. However, abiotic stresses have more adverse effects on crop yield and crop survival. Hitherto, tolerant plants were mainly produced by classical breeding techniques. Success in breeding for better adapted varieties to abiotic and biotic stresses depends on the concerted efforts of various research domains including plant and cell physiology, molecular biology, genetics and breeding. However, such process is time consuming. The production of transgenic plants by genetic engineering allows a much faster progress. This review focuses on stress tolerance on tobacco. Tolerance to salt, drought, water stress, low and high temperature, heavy metals, pathogens and pests were enhanced via overexpression of specific stress related genes from different sources.

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Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco

Cited by 101 publications

References 35 publications

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

Down-Regulation of ZmEXPB6 (Zea mays β-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves of Z. mays L.

Current trends in genetic manipulations to enhance abiotic and biotic stresses in tobacco

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