Timely understanding and quantitative analysis of the changing trend in natural ecosystems in arid and semi-arid areas and their response to the ecological water supply process are of great significance for maintaining the health of oasis ecosystems. Taking the Eichmann Lake wetland of the Aksu River Basin in Xinjiang as the research area, the temporal-spatial distribution characteristics of the lake and the response of ecological water in recent years were studied based on remote sensing images and monitoring data. The results show that: (1) The water surface area of Eichmann Lake is shrinking, from 61.57 km2 in 1996 to 27.76 km2 in 2020. The changes in water surface area have experienced three stages: rapid decline, slow decline, and slow recovery. After the ecological water supply, the water surface area has obvious seasonal changes with hysteresis; (2) In areas with a low average water level, the ecological water supply has a significant impact on the groundwater level. The higher the water supply is, the higher the groundwater level will be. There is a significant lag effect between the change in the groundwater level and the response of the ecological water supply, which is 1–2 months; (3) The response characteristics of different natural vegetation to the ecological water supply were different in interannual, seasonal, and spatial contexts. The response of Populus euphratica to the ecological water supply is obvious, and its growth is the best within the range of 100–500 m from the water supply outlet. This research can provide the basis for the rational allocation of the Aksu River Basin’s water resources, and also act as a valuable reference for the restoration and reconstruction of surrounding vegetation in the Aksu River irrigation area.