Drug-induced Thrombocytopenia

Miescher, P. A.; Graf, Johann

doi:10.1016/s0308-2261(21)00193-4

Cited by 32 publications

(1 citation statement)

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“…Drug-induced thrombocytopenia is one of the major adverse effects of anti-cancer drugs. Two main different causes of drug-induced thrombocytopenia can be distinguished: direct cytotoxicity of the drug and immune-mediated thrombocytopenia (by the formation of immune complexes or of autoantibodies; Miescher and Graf, 1980). The first cause is characterized by direct action of xenobiotics upon platelet progenitorscolony forming unit-megakaryocytes (CFU-MK)-or circulating platelets.…”

Section: Commentary Background Informationmentioning

confidence: 99%

CFU‐MK Assay for Acute Thrombocytopenia

Parent‐Massin

Sibiril

2008

CP Toxicology

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In this unit, protocols to culture human platelet progenitors (Colony Forming Unit-Megakaryocyte; CFU-M) with or without toxicants are described. Platelet progenitors are obtained from human umbilical cord blood. After separation of mononuclear cells, the cell suspension can be cryopreserved or plated immediately. Megakaryocytes are identified by immunocytochemistry. Test chemicals are added to the culture medium before cells are plated. Megakaryocytes are scored after 12 days of culture. IC(10), IC(50) and IC(90) can be calculated by comparison to control cultures. A predictive model is proposed to evaluate the hazard of thrombocytopenia induced by chemicals. When IC(50) and IC(90) are below C(max) in humans, the likelihood of thrombocytopenia is strong.

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Section: Commentary Background Informationmentioning

confidence: 99%