Dry-reagent gold nanoparticle-based lateral flow biosensor for the simultaneous detection of Vibrio cholerae serogroups O1 and O139

Lateral flow assays: Principles, designs and labels

“…Most of them are specific and sensitive, they take a lot of time, and require expensive instrumentation and specialized personnel. When compared to conventional analytical methods, lateral flow assays have many advantages in terms of low cost, easy operation, friendly use, on site response, rapid and visual results based on the naked eye [1][2][3]. Moreover, LFAs play an important role in detecting several compounds by obtaining results in a short time with minimal labor.…”

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139

“…cholerae O1 and O139 was developed and evaluated as a POCT method in this study. Quantification detection of two targets in one strip by immobilizing two specific detection antibodies in adjacent positions of the nitrocellulose membrane was achieved for the first time, a feature that is distinct from single-target quantitation detection [28,29], multiplexed qualitative detection [26], and multiplexed quantitation detection via simple assemblage of many single-target strips in a disc [30]. Without the intensified signals from the contribution of the dependent C line as that of the single-target assay (VchO1-UPT-LF and VchO139-UPT-LF), the sensitivity of the Vch-UPT-LF assay’s two channels for detecting V .…”

“…The sensitivity of traditional POCT methods such as the agglutination test [21–23] is too low for reliable application, while an easily quenched fluorescent group makes fluorescent assays unstable [24], although they are sufficiently sensitive. An immune-chromatographic strip test that uses colloidal gold nanoparticles as bio-labels is a common POCT method; however, the results of the test, especially for samples with low concentrations of target analytes or interfering contaminants, are difficult to assess because they are based on observations using the naked eye [25,26]. Therefore, rapid, simple, sensitive, and accurate POCT detection methods are still needed.…”

Emerging (Bio)Sensing Technology for Assessing and Monitoring Freshwater Contamination - Methods and Applications

“…By Recently, an ultrasensitive microcantilever-based biosensor with dynamic force microscopy for the detection of Vibrio cholera O1 with a detection limit of 1×10 3 CFU mL -1 and a mass sensitivity, Δm/ΔF, of 146.5 pg/Hz was reported by Sungkanak et al 2010. A dry-reagent www.intechopen.com gold nanoparticle-based lateral flow biosensor for the simultaneous detection of Vibrio cholerae serogroups O1 and O139, based on immunochromatographic principle, was also developed by (Yu et al, 2011).…”