DsRNA-based pesticides: Considerations for efficiency and risk assessment

Dalakouras, Athanasios; Koidou, Venetia; Papadopoulou, Kalliope

doi:10.1016/j.chemosphere.2024.141530

Cited by 17 publications

(1 citation statement)

References 143 publications

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“…However, it is interesting to point out that in the EU, dsRNAs are considered chemical pesticides; in the USA, they are biochemical pesticides; and in Australia, they are considered agricultural chemicals. Recently, the Organization for Economic Co-operation and Development (OECD) has produced guidance documents on the risk assessment of dsRNA products related to the environment and human health [ 485 , 509 ]. Well-defined, perhaps tailor-made, risk assessment protocols may be needed to comprehensively evaluate several factors (e.g., formulations, epigenetic modifications, fate of dsRNA in soil, surface water and sediment, leaf tissues, trophic chain exposure, off-target and non-target organisms) involved in the use of dsRNA-based products [ 509 ].…”

Section: Perspectives On Spray-induced Gene Silencing (Sigs) As a New...mentioning

confidence: 99%

Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

Spada,

Pugliesi,

Fambrini

et al. 2024

IJMS

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The necrotrophic plant pathogenic fungus Botrytis cinerea (Pers., 1794), the causative agent of gray mold disease, causes significant losses in agricultural production. Control of this fungal pathogen is quite difficult due to its wide host range and environmental persistence. Currently, the management of the disease is still mainly based on chemicals, which can have harmful effects not only on the environment and on human health but also because they favor the development of strains resistant to fungicides. The flexibility and plasticity of B. cinerea in challenging plant defense mechanisms and its ability to evolve strategies to escape chemicals require the development of new control strategies for successful disease management. In this review, some aspects of the host-pathogen interactions from which novel and sustainable control strategies could be developed (e.g., signaling pathways, molecules involved in plant immune mechanisms, hormones, post-transcriptional gene silencing) were analyzed. New biotechnological tools based on the use of RNA interference (RNAi) are emerging in the crop protection scenario as versatile, sustainable, effective, and environmentally friendly alternatives to the use of chemicals. RNAi-based fungicides are expected to be approved soon, although they will face several challenges before reaching the market.

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Section: Perspectives On Spray-induced Gene Silencing (Sigs) As a New...mentioning

confidence: 99%

Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

Spada,

Pugliesi,

Fambrini

et al. 2024

IJMS

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Biosafety aspects of RNAi‐based pests control

Chen,

De Schutter

2024

Pest Management Science

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While the overuse of classical chemical pesticides has had a detrimental impact on the environment and human health, the discovery of RNA interference (RNAi) offered the opportunity to develop new and sustainable approaches for pest management. RNAi is a naturally occurring regulation and defense mechanism that can be exploited to effectively protect crops by silencing key genes affecting the growth, development, behavior or fecundity of pests. However, as with all technologies, there is a range of potential risks and challenges associated with the application of RNAi, such as dsRNA stability, the potential for off‐target effects, the safety of non‐target organisms, and other application challenges. A better understanding of the molecular mechanisms involved in RNAi and in‐depth discussion and analysis of these associated safety risks, is required to limit or mitigate potential adverse effects.This article is protected by copyright. All rights reserved.

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Plant‐derived artificial miRNA effectively reduced the proliferation of aphid (Aphidoidea) through spray‐induced gene silencing

Wang,

Li,

Zhu

et al. 2024

Pest Management Science

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BACKGROUNDAphids (Hemiptera: Aphididae) are notorious sap‐sucking insects that rampantly threaten agricultural production worldwide. Current management against aphids in the field heavily relies on chemical pesticides, which makes economical and eco‐friendly methods urgently needed. Spray‐induced gene silencing (SIGS) offers a powerful and precise approach to pest management. However, the high costs and instability of double‐stranded RNA (dsRNA) regulators applied for downstream RNA interference (RNAi) still limit this strategy. It remains uncertain if RNAi regulators applied in SIGS could extend to small RNA (sRNA), especially miRNA.RESULTSWe choose two sRNA sequences, miR‐9b and miR‐VgR, whose corresponding targets ABCG4 and VgR are both essential for aphid growth and development. The efficacy of these sequences was initially verified by chemically synthetic single‐stranded RNA (syn‐ssRNA). Through spray treatment, we observed a significantly decreased survival number and increased abnormality rate of green peach aphids fed on the host under laboratory conditions. Based on our previous study, we generated transgenic plants expressing artificial miR‐9b (amiR‐9b) and miR‐VgR (amiR‐VgR). Remarkably, plant‐derived amiRNA exerted potent and long‐lasting inhibitory efficacy with merely one percent concentration of chemical synthetics. Notably, the simultaneous application of amiR‐9b and amiR‐VgR exhibited superior inhibitory efficacy.CONCLUSIONWe explored the potential use of sRNA‐based biopesticide through SIGS while investigating the dosage requirements. To optimize this strategy, the utilization of plant‐derived amiRNA was proposed. The results suggested that attributed to stability and durability, deploying amiRNA in pest management is a potential and promising solution for the field application.This article is protected by copyright. All rights reserved.

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DsRNA-based pesticides: Considerations for efficiency and risk assessment

Cited by 17 publications

References 143 publications

Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

Biosafety aspects of RNAi‐based pests control

Plant‐derived artificial miRNA effectively reduced the proliferation of aphid (Aphidoidea) through spray‐induced gene silencing

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