dsRNA interference on expression of a RNA-dependent RNA polymerase gene of Bombyx mori cytoplasmic polyhedrosis virus

Pan, Zhijuan; Gao, Kun; Hou, Chengxiang; Wu, Ping; Qin, Guangxing; Geng, Tao; Guo, Xijie

doi:10.1016/j.gene.2015.03.069

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…Later, the siRNAs recruit and guide the RNA-induced silencing complex (RISC) to the complementary RNA sequence, which is then cleaved and degraded [ 22 – 25 ]. The recruitment of this mechanism has led to the development of new methods for insect pest control, manipulation of insect disease vectors and disease management of beneficial insects [ 26 – 33 ]. Importantly, large-scale field implementation of this technology has proven to be successful in honey bees [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population

Bona¹,

Chitolina²,

Fermino³

et al. 2016

Parasites Vectors

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BackgroundMosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. The emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and Zika. Therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. Herein we demonstrated the use of RNA interference (RNAi) to increase susceptibility of adult mosquitoes to a widely used pyrethroid insecticide.MethodsExperiments were performed on a field-collected pyrethroid resistant strain of Ae. aegypti (Rio de Janeiro; RJ). Larvae from the resistant Ae. aegypti population were soaked with double-stranded RNAs (dsRNAs) that correspond either to voltage-gate sodium channel (VGSC), P-glycoprotein, or P450 detoxification genes and reared to adulthood. Adult mortality rates in the presence of various Deltamethrin pyrethroid concentrations were used to assess mosquito insecticide susceptibility.ResultsWe characterized the RJ Ae. aegypti strain with regard to its level of resistance to a pyrethroid insecticide and found that it was approximately 6 times more resistant to Deltamethrin compared to the laboratory Rockefeller strain. The RJ strain displayed a higher frequency of Val1016Ile and Phe1534Cys substitutions of the VGSC gene. The resistant strain also displayed a higher basal expression level of VGSC compared to the Rockefeller strain. When dsRNA-treated mosquitoes were subjected to a standard pyrethroid contact bioassay, only dsRNA targeting VGSC increased the adult mortality of the pyrethroid resistant strain. The dsRNA treatment proved effective in increasing adult mosquito susceptibility over a range of pyrethroid concentrations and these results were associated with dsRNA-specific small interfering RNAs in treated adults, and the corresponding specific down regulation of VGSC gene expression level. Finally, we demonstrated that the efficiency of our approach was further improved by ‘tiling’ along the VGSC gene in order to identify the most potent dsRNA sequences.ConclusionsThese results demonstrate that dsRNA applied to mosquito larvae retains its biological activity into adulthood. Thus, the RNAi system reported here could be a useful approach to control the widespread insecticide resistance in mosquitoes and other insect vectors of human diseases.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1634-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population

Bona¹,

Chitolina²,

Fermino³

et al. 2016

Parasites Vectors

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“…The results showed that injecting dsRNA into fifth-instar larvae can reduce BmCPV-RDRP expression by 93% after virus infection. 58 At the same time, RNAi also downregulated the expression of other viral genes. RNAi technology based on engineered plasmids can produce dsRNA steadily and continuously in cells, thereby achieving long-term inhibition of target genes.…”

Section: Bombyx Mori Cytoplasmic Polyhedrosis Virusmentioning

confidence: 99%

Common strategies in silkworm disease resistance breeding research

Dong

Pan

2023

Pest Management Science

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The silkworm, which is considered a model invertebrate organism, was the first insect used for silk production in human history and has been utilized extensively throughout its domestication. However, sericulture has been plagued by various pathogens that have caused significant economic losses. To enhance the resistance of a host to its pathogens,numerous strategies have been developed. For instance, gene-editing techniques have been applied to a wide range of organisms, effectively solving a variety of experimental problems. This review focuses on several common silkworm pests and their pathogenic mechanisms, with a particular emphasis on breeding for disease resistance to control multiple types of silkworm diseases. The review also compares the advantages and disadvantages of transgenic technology and gene-editing systems. Finally, the paper provides a brief summary of current strategies used in breeding silkworm disease resistance, along with a discussion of the establishment of existing technologies and their future application prospects.

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“…(Pak & Fire, 2007). Many viruses also encode RdRps, which allow for the proliferation of viral RNAs (Pan et al, 2016).…”

Section: Tablementioning

confidence: 99%

Implementing the sterile insect technique with RNA interference – a review

Darrington

Dalmay

Morrison

et al. 2017

Entomologia Exp Applicata

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We review RNA interference (RNAi) of insect pests and its potential for implementing sterile insect technique (SIT)‐related control. The molecular mechanisms that support RNAi in pest species are reviewed in detail, drawing on literature from a range of species including Drosophila melanogaster Meigen and Homo sapiens L. The underlying genes that enable RNAi are generally conserved across taxa, although variance exists in both their form and function. RNAi represents a plausible, non‐GM system for targeting populations of insects for control purposes, if RNAi effector molecules can be delivered environmentally (eRNAi). We consider studies of eRNAi from across several insect orders and review to what extent taxonomy, genetics, and differing methods of double‐stranded (ds) RNA synthesis and delivery can influence the efficiency of gene knockdown. Several factors, including the secondary structure of the target mRNA and the specific nucleotide sequence of dsRNA effector molecules, can affect the potency of eRNAi. However, taxonomic relationships between insects cannot be used to reliably forecast the efficiency of an eRNAi response. The mechanisms by which insects acquire dsRNA from their environment require further research, but the evidence to date suggests that endocytosis and transport channels both play key roles. Delivery of RNA molecules packaged in intermediary carriers such as bacteria or nanoparticles may facilitate their entry into and through the gut, and enable the evasion of host defence systems, such as toxic pH, that would otherwise attenuate the potential for RNAi.

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dsRNA interference on expression of a RNA-dependent RNA polymerase gene of Bombyx mori cytoplasmic polyhedrosis virus

Cited by 8 publications

References 39 publications

Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population

Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population

Common strategies in silkworm disease resistance breeding research

Implementing the sterile insect technique with RNA interference – a review

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