DTT–Au NCs Interact with DNA to Form Raspberry‐Like Particles

Zhang, Lixia; Li, Zhenhua; Jing, Jiangbo; Ding, Han; Chen, Zhijun

doi:10.1002/ppsc.201800517

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…Li et al used the hybrids doped by tryptophan to load the plasmids expressing green fluorescent protein into the cells and expressed the green fluorescent protein in the yeast cells 100 . Zhang et al used the DTT protected gold nanoclusters to load the plasmids expressing green fluorescent protein into the cells and expressed the green fluorescent protein in the yeast cells 101 . It also opened up a new way for gene therapy by using protein Au NCs instead of viruses.…”

Section: Study On the Application Of Protein Au Ncsmentioning

confidence: 99%

Nanotheranostic Application of Fluorescent Protein-Gold Nanocluster Hybrid Materials: A Mini-review

Ding¹,

Chen²

2021

Nanotheranostics

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The gold nanoclusters (Au NCs) are a special kind of gold nanomaterial containing several gold atoms. Because of their small size and large surface area, Au NCs possess macroscopic quantum tunneling and dielectric domain effects. Furthermore, Au NCs fluorescent materials have longer luminous time and better photobleaching resistance compared with other fluorescent materials. The synthetic process of traditional Au NCs is complicated. Traditional Au NCs are prepared mainly by using polyamide amine type dendrites, and sixteen alkyl trimethylamine bromide or sulfhydryl small molecule as stabilizers. They are consequently synthesized by the reduction of strong reducing agents such as sodium borohydride. Notably, these materials are toxic and environmental-unfriendly. Therefore, there is an urgent need to develop more effective methods for synthesizing Au NCs via a green approach. On the other hand, the self-assembly of protein gold cluster-based materials, and their biomedical applications have become research hotspots in this field. We have been working on the synthesis, assembly and application of protein conjugated gold clusters for a long time. In this review, the synthesis and assembly of protein-gold nanoclusters and their usage in cell imaging and other medical research are discussed.

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Section: Study On the Application Of Protein Au Ncsmentioning

confidence: 99%

Nanotheranostic Application of Fluorescent Protein-Gold Nanocluster Hybrid Materials: A Mini-review

Ding¹,

Chen²

2021

Nanotheranostics

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“…Nucleic acids represent another big class of biomolecules which are extensively exploited in the field of nanobiomaterials. Nanotechnologies based on DNA strands have become highly demanding, as they can provide high-level tasks such as gene therapy and DNA-target-recognition techniques [ 107 , 108 ]. Recently, DNA was also tested as a promising ligand for AuNCs.…”

Section: Design and Application Of Auncs For Bioimagingmentioning

confidence: 99%

Luminescent Gold Nanoclusters for Bioimaging: Increasing the Ligand Complexity

et al. 2023

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Fluorescence, and more in general, photoluminescence (PL), presents important advantages for imaging with respect to other diagnostic techniques. In particular, detection methodologies exploiting fluorescence imaging are fast and versatile; make use of low-cost and simple instrumentations; and are taking advantage of newly developed powerful, low-cost, light-based electronic devices, such as light sources and cameras, used in huge market applications, such as civil illumination, computers, and cellular phones. Besides the aforementioned simplicity, fluorescence imaging offers a spatial and temporal resolution that can hardly be achieved with alternative methods. However, the two main limitations of fluorescence imaging for bio-application are still (i) the biological tissue transparency and autofluorescence and (ii) the biocompatibility of the contrast agents. Luminescent gold nanoclusters (AuNCs), if properly designed, combine high biocompatibility with PL in the near-infrared region (NIR), where the biological tissues exhibit higher transparency and negligible autofluorescence. However, the stabilization of these AuNCs requires the use of specific ligands that also affect their PL properties. The nature of the ligand plays a fundamental role in the development and sequential application of PL AuNCs as probes for bioimaging. Considering the importance of this, in this review, the most relevant and recent papers on AuNCs-based bioimaging are presented and discussed highlighting the different functionalities achieved by increasing the complexity of the ligand structure.

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“…In particular, this constituted a promising sensor-free diagnostic platform for the detection of cancer. Chen and co-workers showed that the interaction of DNA with dithiothreitol (DTT)-conjugated gold nanoclusters (Au NCs) results in the formation of 'raspberry-like' particles with potential gene delivery application [99]. The novelty of the 'raspberry-like' structures lay in their biocompatible 'shield', which protected the capped DNA from enzymatic degradation.…”

Section: Dna-metal Nanoparticle Architecturesmentioning

confidence: 99%

Molecular architectonics of DNA for functional nanoarchitectures

Ghosh¹,

Datta²,

Govindaraju³

2020

Beilstein J. Nanotechnol.

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DNA is the key biomolecule central to almost all processes in living organisms. The eccentric idea of utilizing DNA as a material building block in molecular and structural engineering led to the creation of numerous molecular-assembly systems and materials at the nanoscale. The molecular structure of DNA is believed to have evolved over billions of years, with structure and stability optimizations that allow life forms to sustain through the storage and transmission of genetic information with fidelity. The nanoscale structural characteristics of DNA (2 nm thickness and ca. 40–50 nm persistence length) have inspired the creation of numerous functional patterns and architectures through noncovalent conventional and unconventional base pairings as well as through mutual templating-interactions with small organic molecules and metal ions. The recent advancements in structural DNA nanotechnology allowed researchers to design new DNA-based functional materials with chemical and biological properties distinct from their parent components. The modulation of structural and functional properties of hybrid DNA ensembles of small functional molecules (SFMs) and short oligonucleotides by adapting the principles of molecular architectonics enabled the creation of novel DNA nanoarchitectures with potential applications, which has been termed as templated DNA nanotechnology or functional DNA nanoarchitectonics. This review highlights the molecular architectonics-guided design principles and applications of the derived DNA nanoarchitectures. The advantages and ability of functional DNA nanoarchitectonics to overcome the trivial drawbacks of classical DNA nanotechnology to fulfill realistic and practical applications are highlighted, and an outlook on future developments is presented.

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DTT–Au NCs Interact with DNA to Form Raspberry‐Like Particles

Cited by 4 publications

References 26 publications

Nanotheranostic Application of Fluorescent Protein-Gold Nanocluster Hybrid Materials: A Mini-review

Nanotheranostic Application of Fluorescent Protein-Gold Nanocluster Hybrid Materials: A Mini-review

Luminescent Gold Nanoclusters for Bioimaging: Increasing the Ligand Complexity

Molecular architectonics of DNA for functional nanoarchitectures

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