Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic framework

Li, Ningning; Huang, Xing; Sun, Duanping; Wang, Yu; Tan, Weiguo; Luo, Zhengxiu; Chen, Zuanguang

doi:10.1007/s00604-018-3081-2

Cited by 47 publications

(19 citation statements)

References 32 publications

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“…This emphasizes the need for a diagnostic method that detects only live bacilli. Some other new technologies for TB diagnosis have recently emerged, such as surface plasmon resonance spectroscopy [41], immuno-PCR (a combination of ELISA and PCR) [42,43], voltammetric assay [44,45], aptasensor [46,47], immuno-nanosensor [48], and electrochemiluminescence immunoassay [49]. These methods are also applied to secretory proteins (i.e., antigens) as TB markers, but culture, which takes time, is still required to obtain adequate amounts of antigen [50].…”

Section: Discussionmentioning

confidence: 99%

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

Wang

Takeuchi²,

Jain

et al. 2020

EBioMedicine

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Background Nucleic acid amplification tests (NAATs) are widely used to diagnose tuberculosis (TB), but cannot discriminate live bacilli from dead bacilli. Live bacilli can be isolated by culture methods, but this is time-consuming. We developed a de novo TB diagnostic method that detects only live bacilli with high sensitivity within hours. Methods A prospective study was performed in Taiwan from 2017 to 2018. Sputum was collected consecutively from 1102 patients with suspected TB infection. The sputum was pretreated and heated at 46°C for 1 h to induce the secretion of MPT64 protein from live Mycobacterium tuberculosis . MPT64 was detected with our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide (thio-NAD) cycling. We compared our data with those obtained using a culture test (MGIT), a smear test (Kinyoun staining), and a NAAT (Xpert). Findings The limit of detection for MPT64 in our culture-free ultrasensitive ELISA was 2.0 × 10 −19 moles/assay. When the criterion for a positive response was set as an absorbance value ≥17 mAbs, this value corresponded to ca . 330 CFU/mL in the culture method – almost the same high-detection sensitivity as the culture method. To confirm that MPT64 is secreted from only live bacilli, M. bovis BCG was killed using 8 μg/mL rifampicin and then heated. Following this procedure, our method detected no MPT64. Our rapid ultra-sensitive ELISA-based method required only 5 h to complete. Comparing the results of our method with those of culture tests for 944 specimens revealed a sensitivity of 86.9% (93/107, 95% CI: 79.0–92.7%) and a specificity of 92.0% (770/837, 95% CI: 89.9–93.7%). The performance data were not significantly different (McNemar's test, P = 0.887) from those of the Xpert tests. In addition, at a ≥1+ titer in the smear test, the positive predictive value of our culture-free ultrasensitive ELISA tests was in a good agreement with that of the culture tests. Furthermore, our culture-free ultrasensitive ELISA test had better validity for drug effectiveness examination than Xpert tests because our test detected only live bacilli. Interpretation Our culture-free ultrasensitive ELISA method detects only live TB bacilli with high sensitivity within hours, allowing for rapid diagnosis of TB and monitoring drug efficacy. Funding Matching Planner Program from JST (VP29117939087), the A-STEP Program from JST (AS3015096U), Waseda University grants for Specific Research Projects (2017A-015 and 2019C-123), the Precise Measurement Technology Promotion Foundation to E.I.

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Section: Discussionmentioning

confidence: 99%

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

Wang

Takeuchi²,

Jain

et al. 2020

EBioMedicine

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“…Aptamer-based electrochemical biosensors based on UiO-66-NH 2 are also a hot research topic. Li et al employed UiO-66-NH 2 as a carrier for detecting Mycobacterium tuberculosis antigen MPT64 in serum by immobilizing gold nanoparticles, aptamer, and horseradish peroxidase as signal probes ( Li et al, 2018 ). To improve the sensor’s recognition performance, Zhu et al combined graphene oxide and UiO-66 to enhance the aptamer’s affinity to the carrier ( Zhu et al, 2020 ).…”

Section: Application Of Uio-66-based Electrochemical Biosensorsmentioning

confidence: 99%

Research Progress of UiO-66-Based Electrochemical Biosensors

Zhang

et al. 2022

Front. Chem.

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UiO-66, as a member of the MOFs families, is widely employed in sensing, drug release, separation, and adsorption due to its large specific surface area, uniform pore size, easy functionalization, and excellent stability. Especially in electrochemical biosensors, UiO-66 has demonstrated excellent adsorption capacity and response signal, which significantly improves the sensitivity and specificity of detection. However, the existing application research remains in its infancy, lacking systematic methods, and recycling utilization and exclusive sensing of UiO-66 still require further improvement. Therefore, one of the present research objectives is to explore the breakthrough point of existing technologies and optimize the performance of UiO-66-based electrochemical biosensors (UiO-66-EBs). In this work, we summarized current experimental methods and detection mechanisms of UiO-66-EBs in environmental detection, food safety, and disease diagnosis, analyzed the existing problems, and proposed some suggestions to provide new ideas for future research.

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“…Its oxidation current was proportional to the quantity of the double-stranded DNA scaffolds on the electrode surface. Zr MOFs synthesized from ZrCl 4 and 2-aminoterephtalic acid and saturated with Pb 2+ or Cd 2+ ions were successfully applied for the determination of antibiotics [116,127]. The Zr MOFs particles were modified with appropriate aptamers and saturated with the metal ions.…”

Section: Signals Of the Redox Labels And Indicatorsmentioning

confidence: 99%

“…In addition to the high selectivity of aptamer-analyte interaction, the use of MOFs offers amplification of the signal via implementation of catalytic cycles. For this purpose, horseradish peroxidase [127,137] was used in the framework of the traditional competitive and sandwich assay with hydroquinone substrate and redox detection of benzoquinone formed in the reaction. Besides, catalytic reaction of H 2 O 2 oxidation catalyzed by the MOFs bearing noble metals has been proposed for thrombospondin-1 [146] and led [133] determination.…”

Section: Other Detection Techniquesmentioning

confidence: 99%

Electrochemical Aptasensors Based on Hybrid Metal-Organic Frameworks

Evtugyn

Belyakova

Porfireva

et al. 2020

Sensors

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Metal-organic frameworks (MOFs) offer a unique variety of properties and morphology of the structure that make it possible to extend the performance of existing and design new electrochemical biosensors. High porosity, variable size and morphology, compatibility with common components of electrochemical sensors, and easy combination with bioreceptors make MOFs very attractive for application in the assembly of electrochemical aptasensors. In this review, the progress in the synthesis and application of the MOFs in electrochemical aptasensors are considered with an emphasis on the role of the MOF materials in aptamer immobilization and signal generation. The literature information of the use of MOFs in electrochemical aptasensors is classified in accordance with the nature and role of MOFs and a signal mode. In conclusion, future trends in the application of MOFs in electrochemical aptasensors are briefly discussed.

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Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic framework

Cited by 47 publications

References 32 publications

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

Research Progress of UiO-66-Based Electrochemical Biosensors

Electrochemical Aptasensors Based on Hybrid Metal-Organic Frameworks

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