Dual aptamer-functionalized silica nanoparticles for the highly sensitive detection of breast cancer

Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy

“…Aptamers, single-stranded RNA or DNA oligonucleotide selected by the systematic evolution of ligands by exponential enrichment (SELEX) methodology, interact with targets by forming special three-dimensional conformations. Since the concept of aptamer was first proposed by Ellington in 1990 [7], it has been presented as a regnant bioreceptor for the construction of various aptasensors to detect ions [8,9], proteins [1,10], and cells [11,12], as aptamers possess high affinity, exquisite specificity, reduced immunogenicity, increased stability, and good reproducibility. Moreover, aptamers could be massively synthesized and easily modified via chemical processes, which is more costeffective [13].…”

“…Subsequently, an aptamer (anti-MUC1 S2.2) has been confirmed as a functional molecule, specifically binding to a common sequence (APDTRPAPG) of the VTR region of MUC1. To date, this MUC1 and aptamer binding reaction has led to discerning diagnostic aptasensors for detecting and monitoring tumor progression [1,[10][11][12]. In particular, the presence of divalent metal ions (e.g., Mg 2+ , Ca 2+ ) was indispensable for an aptamer to form the characteristic secondary structure, contributing to the selection process of aptamers and regeneration of aptasensors, in contrast to most existing biosensors [11].…”

Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

“…Mucin1 is both a potential cancer biomarker and an attractive target for cancer immunotherapy [61,62]. Compared with other Apt MUC1 -modified NPs used for the detection of tumor cells, the preparation of our Apt MUC1 -T 30 -CuO/Cu 2 O NPs (label-free) and the sensing platform are relatively simple [63][64][65][66]. We believe that our proposed assay holds great potential for the detection of Mucin1 overexpression in tumor cells, allowing highly accurate and reliable diagnoses of Mucin1-related cancers.…”

An Overview of Naturally Synthesized Metallic Nanoparticles

“…Silica nanopraticles (Dual-SiNPs) which was synthesised using dual aptamer modifier have excellent sensitive detection against two breast cancer cell such as mucin 1 (MUC1) (+) and human epidermal growth factor receptor 2 (HER2) (+) cell lines at the concentration of 1cell/100µL. This indicates its role in diagnosis and prognosis of breast cancer (Jo et al, 2015).…”