Dual biosyntheses of poly[(R)-3-hydroxybutyric acid] and silk protein for the fabrication of biofunctional bioplastic

Numata, Keiji; Doi, Yoshiharu

doi:10.1038/pj.2011.27

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…2−4 Polyhydroxyalkanoates (PHAs) are a family of biopolyesters produced intracellularly in a wide variety of microorganisms and serve as a carbon and energy storage function. 5 Currently, approximately 150 different hydroxyalkanoate monomers have been identified. 6 PHB is the most studied member of the PHA family, its monomer hydroxybutyric acid (3-HBA) is chemically identical to mammalian HBA produced as one of the ketone bodies during prolonged starvation and under diabetic conditions, and is recognized by mammalian enzymes.…”

Section: ■ Introductionmentioning

confidence: 99%

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BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration

Chan¹,

Russell

Marçal³

et al. 2013

Biomacromolecules

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This study reports on the superior suitability of Polyhydroxybutyrate-polyethylene glycol hybrid polymers biosynthesised by Cupriavidus necator over PHB as biomaterials for tissue engineering. Incorporation of PEG106 (DEG) during PHB biosynthesis reduced crystallinity, molecular weight, and hydrophobicity while improving mechanical properties. In vitro olfactory ensheathing cell (OEC) proliferation was enhanced by cultivation on PHB-b-DEG films. Cultivation on PHB and PHB-b-DEG films showed no cytotoxic responses and cell viability and membrane integrity was sustained. PHB-b-DEG films promoted OECs entering into the DNA replication (S) phase and mitotic (G2-M) phase during the cell growth cycle and apoptosis was low. This study also confirmed an association between the level of neurite-outgrowth inhibitory protein (Nogo) and receptor pair Ig-like receptor B (PirB) expression and cell proliferation, both being down-regulated in cells grown on hybrid films when compared with PHB and asynchronous growth. Thus, DEG-terminated PHB-based biomaterials have great potential as biological scaffolds supporting nerve repair.

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Section: ■ Introductionmentioning

confidence: 99%

“…Polyhydroxyalkanoates (PHAs) are a family of biopolyesters produced intracellularly in a wide variety of microorganisms and serve as a carbon and energy storage function . Currently, approximately 150 different hydroxyalkanoate monomers have been identified .…”

Section: Introductionmentioning

confidence: 99%

BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration

Chan¹,

Russell

Marçal³

et al. 2013

Biomacromolecules

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“…Thus, PHAs have a variety of possible applications, that could replace traditional plastics derived from petroleum [ 11 ]. Some of the possible applications are highlighted in previous studies [ 12 , 13 ] and include: packaging, medical uses [ 14 ], agricultural uses, and in carbon nanotubes [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach

et al. 2013

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BackgroundPolyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a short-chain-length PHA with interesting chemical and physical properties. Large scale production of PHB is not wide-spread mainly due to the downstream processing of bacterial cultures to extract the PHB. Secretion of PHB from Escherichia coli could reduce downstream processing costs. PHB are non-proteinaceous polymers, hence cannot be directly targeted for secretion. Phasin, PhaP1, is a low molecular weight protein that binds to PHB, reducing PHB granule size. In this study PHB is indirectly secreted with PhaP1 from E. coli via type I secretion using HlyA signal peptides.ResultsThis study demonstrated the successful secretion of phasin and phasin bound PHB outside of the cell and into the culture medium. The secretion of PHB was initiated between 24 and 48 h after induction. After 48 h of culturing, 36% of the total PHB produced in the secreting strain was collected in the secreted fraction and 64% remained in the internal fraction. To further support the findings of this study, the PHB secretion phenomenon was observed using SEM.ConclusionsFrom this study, the ability to use type I secretion to: 1) secrete phasin and 2) successfully secrete PHB has been shown.

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“…We previously reported various recombinant silk-like peptides containing ligand molecules that facilitated selective delivery to target cells. 56,[61][62][63][64][65][66] Complexes of recombinant silk molecules containing one of the CPPs, ppTG1 peptide, with plasmid DNA (pDNA) were designed for pDNA delivery. 62 The peptide composed of dimeric ppTG1 and a cationic sequence formed a globular pDNA complex of o100 nm in hydrodynamic diameter that was as efficiently transfected as the transfection regent Lipofectamine 2000.…”

Section: Poly(amino Acid)/polypeptide As Functional Materials: Targetmentioning

confidence: 99%

Poly(amino acid)s/polypeptides as potential functional and structural materials

Numata

2015

Polym J

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Poly(amino acids) and polypeptides have the potential to contribute significantly to a biomass-based and sustainable society, due to their biomass origin, functionality, and unique physical properties. To realize amino acid-based polymers as eco-friendly alternatives for petroleum materials, the synthesis of poly(amino acid)s/polypeptides through an environmentally friendly process is needed. In this focus review, the author summarizes the recent progress of chemo-enzymatic polymerization, which is a green and atom-economical reaction that provides new insight into the design of materials from polypeptides. Additionally, polypeptides can be designed to serve as functional and structural materials. The use of peptides as carriers of nucleic acids for delivery into target cells and organelles is one important application of such functional materials. Studies on polypeptides as structural materials are also reviewed. POLY(AMINO ACID) AS AN ECO-FRIENDLY MATERIALEco-friendly polymeric materials have been designed and processed primarily from bio-based polyesters such as poly(hydroxyalkanoate) 1 and poly(lactic acid), 2,3 due to their plasticity, excellent workability, and biomass origin. 4-12 Poly(hydroxyalkanoate) has several advantages such as the ability to be directly synthesized from various types of biomass, including lignin and carbon dioxide, 13-17 and excellent biodegradability. 18,19 However, because of a lack of toughness, this biopolyester has limited use and application. Development of an engineered biopolymer such as a biomass-derived polyamide is an emerging interest in the field of sustainable chemistry and materials engineering. Biopolyamides (for example, nylon 4) have been investigated as candidates for biomass-based engineering plastics, but their narrow processing window for thermo-forming currently prevents them from being considered for practical purposes. 20 Natural high-performance materials, including spider silk and mussel-derived adhesive (Figure 1), are mainly composed of poly(amino acid)s and a small amount of short peptides, suggesting that poly(amino acid)s could serve as an alternative to the petroleum-derived polymers in support of a sustainable society. Although spider dragline silk is a benchmark in terms of tough materials, scientific and technological advances still cannot produce an artificial dragline silk. To create and develop biomass-based tough polymers like this silk, the synthesis and design of materials from poly(amino acid)s is being investigated.A poly(amino acid) is a polymer composed of amino acids as monomeric units. Structural and functional proteins, polypeptides, peptides and polymers derived from amino acids, that is, poly(β-alanine) and ε-poly(lysine), are classified as poly(amino acid)s. The use of poly(amino acid)s as functional materials has been widely studied, resulting in the development of polypeptides that are bioactive and that can have biological applications such as in tissue engineering, regenerative medicine and drug/gene delivery systems. Poly(amin...

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Dual biosyntheses of poly[(R)-3-hydroxybutyric acid] and silk protein for the fabrication of biofunctional bioplastic

Cited by 6 publications

References 30 publications

BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration

BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration

Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach

Poly(amino acid)s/polypeptides as potential functional and structural materials

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