Dual crosslinking strategy to generate mechanically viable cell-laden printable constructs using methacrylated collagen bioinks

Kajave, Nilabh S.; Schmitt, Trevor; Nguyen, Thuy‐Uyen; Kishore, Vipuil

doi:10.1016/j.msec.2019.110290

Cited by 38 publications

(44 citation statements)

References 41 publications

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“…This was especially true for the WS 2 / PCL/CS scaffolds as a nearly 30% enhancement of the cell viability, as compared with the PCL/CS scaffolds, was observed at Day 7. This is attributed to the WS 2 /PCL/CS scaffold offering better microenvironmental conditions for hMSCs viability 56 . To evaluate the osteoblastic differentiation efficiency in detail, the useful marker of ALP activity was performed.…”

Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 97%

“…5a). The interconnected macro-pore structure promotes cell in-growth and nutrient transportation, and their bioactive components can lead to osteogenesis and angiogenesis 56 . To understand the homogeneity of the chlorophyll-assisted exfoliated WS 2 thin sheets in the WS 2 /PCL/CS composite, non-destructive Raman measurements were used.…”

Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 99%

“…Figure 6d shows that hMSCs had a better adhesion to the WS 2 /PCL/CS scaffold than to the PCL/CS scaffold after 12 h. The authors believe that the WS 2 /PCL/CS scaffold can adsorb more Col I on the surface so enhancing the initial cell attachment ability. Various studies have demonstrated that Col I can regulate the attachment, proliferation, and osteogenic differentiation of stem cells 56 . Overall, the cell adhesion and viability data demonstrate that the chlorophyllassisted exfoliated WS 2 thin sheets did not exhibit any cytotoxic responses to the hMSCs.…”

Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 99%

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Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

et al. 2020

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Due to the population explosion of the 21st century, nearly one billion people are over 64 years of age and bone fracture is one of the most frequent problems facing both sexes because of osteoporosis. However, difficulty in enhancing bone regeneration to repair bone fracture poses challenges and thus, a two-dimensional monolayer material (i.e. tungsten disulfide (WS2)) could be one of the candidates offering a possible solution to the problem. Here, we prepare high-quality monolayer WS2 thin sheets in a large quantity with the assistance of extracted chlorophyll molecules, the natural pigment used in photosynthesis, via a liquid-phase exfoliation method. Then, the exfoliated WS2 sheets were mixed with polycaprolactone (PCL)/calcium silicate (CS) to form a biocompatible WS2-based composite. The in vivo experiments show that the bone regeneration of the WS2-based composite was 120% superior to commercially available mineral trioxide aggregate (MTA) bone cement. Moreover, the mechanical properties of the WS2-based composite exhibited ~300% enhancement over PCL/CS, which is one of the most commonly used bone regeneration materials. Our findings highlight the prospects for the composite of WS2 towards the improvement of bone regeneration applications.

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Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 97%

Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 99%

Section: Physical Properties Of Ws 2 /Pcl/cs Scaffoldmentioning

confidence: 99%

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Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

et al. 2020

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“…The most commonly used stem cells are bone marrow-derived mesenchymal stem cells (BM-MSCs), as they were the first to be isolated [ 28 , 43 , 46 , 47 , 55 , 56 , 63 ]. They have a multilineage differentiation capacity and can differentiate into several cell types, including skin-like cells, i.e., fibroblasts [ 46 ], keratinocytes, endothelial cells, and pericytes [ 68 ].…”

Section: Overview Of Current Research On Extrusion-based Skin Biopmentioning

confidence: 99%

“…Following a biomimetic approach, collagen type I has been proposed as the main component in bioink in several studies [ 3 , 28 , 29 , 30 , 31 , 52 ]. Collagen type I can reproduce a natural environment with Arg–Gly–Asp (RGD) domains and replicate skin porosity and anisotropy.…”

Section: Cell-laden Bioinksmentioning

confidence: 99%

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

Perez‐Valle

Amo

Andı́a

2020

IJMS

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Bioprinting technologies, which have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix components, and other scaffold-like biomaterials, are currently being exploited for the fabrication of human skin in regenerative medicine. We performed a systematic review to appraise the latest advances in 3D bioprinting for skin applications, describing the main cell phenotypes, signaling proteins, and bioinks used in extrusion platforms. To understand the current limitations of this technology for skin bioprinting, we briefly address the relevant aspects of skin biology. This field is in the early stage of development, and reported research on extrusion bioprinting for skin applications has shown moderate progress. We have identified two major trends. First, the biomimetic approach uses cell-laden natural polymers, including fibrinogen, decellularized extracellular matrix, and collagen. Second, the material engineering line of research, which is focused on the optimization of printable biomaterials that expedite the manufacturing process, mainly involves chemically functionalized polymers and reinforcement strategies through molecular blending and postprinting interventions, i.e., ionic, covalent, or light entanglement, to enhance the mechanical properties of the construct and facilitate layer-by-layer deposition. Skin constructs manufactured using the biomimetic approach have reached a higher level of complexity in biological terms, including up to five different cell phenotypes and mirroring the epidermis, dermis and hypodermis. The confluence of the two perspectives, representing interdisciplinary inputs, is required for further advancement toward the future translation of extrusion bioprinting and to meet the urgent clinical demand for skin equivalents.

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Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink

Fisch

Broguière

Finkielsztein

et al. 2021

Adv Funct Materials

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Bioprinting of functional tissues could overcome tissue shortages and allow a more rapid response for treatments. However, despite recent progress in bioprinting, and its outstanding ability to position cells and biomaterials in a precise 3D manner, its success has been limited, due to insufficient maturation of constructs into functional tissue. Here, a novel calcium‐triggered enzymatic crosslinking (CTEC) mechanism for bioinks based on the activation cascade of Factor XIII is presented and utilized for the biofabrication of cartilaginous constructs. Hyaluronan transglutaminase (HA‐TG), an enzymatically crosslinkable material, has shown excellent characteristics for chondrogenesis and builds the basis of the CTEC bioink. The bioink supports tissue maturation with neocartilage formation and stiffening of constructs up to 400 kPa. Bioprinted constructs remain stable in vivo for 24 weeks and bioprinted auricular constructs transform into cartilaginous grafts. A major limitation of the current study is the deposition of collagen I, indicating the maturation toward fibrocartilage rather than elastic cartilage. Shifting the maturation process toward elastic cartilage will therefore be essential in order for the developed bioinks to offer a novel tissue engineered treatment for microtia patients. CTEC bioprinting furthermore opens up use of enzymatically crosslinkable biopolymers and their modularity to support a multitude of tissues.

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Dual crosslinking strategy to generate mechanically viable cell-laden printable constructs using methacrylated collagen bioinks

Cited by 38 publications

References 41 publications

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink

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