Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples

Xu, Ying; Ma, Bach; Chen, Erjing; Yu, Xiaoping; Ye, Zihong; Sun, Chuanxin; Zhang, Mingzhou

doi:10.1016/j.foodchem.2020.127713

Cited by 33 publications

(13 citation statements)

References 32 publications

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“…This combination process was based on our previous work [ 20 ]. We added 1 mg of carboxylic acid EuNPs to a 400-μL MES solution and then added 15-μL EDC (10 mg/mL) to activate after slowly shaking for 30 min at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

“…Europium nanoparticles (EuNPs), also known as Eu (III)-labeled polystyrene nanoparticles, are a new type of fluorescent probe based on lanthanide labeling [ 19 ]. EuNPs are special functional microspheres, and each microsphere contains thousands of fluorescent molecules [ 20 ]. They have the advantages of good stability, high labeling efficiency, and high sensitivity [ 21 ], and they are not harmful to the sample [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Research on Rapid Detection Technology for β2-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen

Liu

Wang

et al. 2022

Foods

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To detect two types of β2-agonist residues at the same time, we coupled two haptens of clenbuterol (CLE) and ractopamine (RAC) to the same carrier protein through diazotization to prepare dimeric artificial antigen, and a fluorescent lateral flow immunoassay method based on europium nanoparticles (EuNP-FLFIA) was established by combining polyclonal antibodies with europium nanoparticles to form probes. Under optimized conditions, the EuNP-FLFIA could simultaneously detect eight aniline-type and one phenol-type β2-agonists, and the limits of detection (LOD) were 0.11–0.19 ng/mL and 0.12 ng/mL, respectively. The recovery rate of this method was 84.00–114.00%. This method was verified by liquid chromatography–tandem mass spectrometry (LC-MS/MS), and the test results were consistent (R2 > 0.98). Therefore, the method established in this study could be used as a high-throughput screening for the efficient and sensitive detection of β2-agonists in food.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research on Rapid Detection Technology for β2-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen

Liu

Wang

et al. 2022

Foods

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“…First, the conjugation process of EuNP-labeled anti-digoxin monoclonal antibody (EuNPs–anti-digoxin mAb) was performed [ 26 ]. Two milligrams of carboxylic EuNPs was dissolved in 800 μL of 2-(N-morpholino) ethanesulfonic acid (MES, 0.05 M, pH 8.2), before adding 30 μL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to activate.…”

Section: Methodsmentioning

confidence: 99%

A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

Chen

et al. 2021

IJERPH

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Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

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“…Numerous analytical methods have been reported for detection of CIT. For example, high-performance liquid chromatography (HPLC), , liquid chromatography with tandem mass spectrometry (LC/MS/MS), − and immunoassays have been developed . These techniques can generate accurate results.…”

Section: Introductionmentioning

confidence: 99%

Double Isothermal Amplification and CRISPR-Cas12a for Sensitive Detection of Citrinin

Zhang

Xue

Gong

et al. 2021

ACS Food Sci. Technol.

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An analytical method is developed for ultrasensitive detection of citrinin using double isothermal amplification and CRISPR-Cas12a. Gold nanoparticles (AuNPs) modified with antigen and thiol-terminated, single-strand DNA (ssDNA) are used as a probe. The antigen-modified AuNPs compete with citrinin to bind to magnetic beads coated with an anticitrinin antibody. After a simple magnetic separation, the AuNPs are collected, and the ssDNA are released after they are washed with a dithiothreitol solution. The ssDNA is first amplified by an exponential amplification reaction and then used as a primer in a subsequent hybridization chain reaction to produce double-stranded DNA (dsDNA) that contains a protospacer adjacent motif to allow recognition by CRISPR-Cas12a. The dsDNA activates the Cas12a-gRNA to cleave a reporter ssDNA to generate a fluorescence signal. The developed analytical method has a low detection limit (0.127 ng mL −1 ) and a wide linear range (0.005−500 μg mL −1 ) for detection of citrinin. For detection of citrinin in oat and flour, recoveries of 97−104% and 105−111% are obtained, respectively. By combining double isothermal amplification with CRISPR-Cas12a, ultrahigh sensitivity and selectivity can be achieved for detection of toxins in food.

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Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples

Cited by 33 publications

References 32 publications

Research on Rapid Detection Technology for β2-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen

Research on Rapid Detection Technology for β2-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen

A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

Double Isothermal Amplification and CRISPR-Cas12a for Sensitive Detection of Citrinin

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