Dual Function of a Novel Bacterium, Slackia sp. D-G6: Detoxifying Deoxynivalenol and Producing the Natural Estrogen Analogue, Equol

Gao, Xiaojuan; Mu, Peiqiang; Zhu, Xunhua; Chen, Xiaoxuan; Tang, Shulin; Wu, Yuting; Miao, Xiang; Wang, Xiaohan; Wen, Jikai; Deng, Yiqun

doi:10.3390/toxins12020085

Cited by 31 publications

(17 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contradictory findings have been reported in different studies about Slackia . For instance, a recent study reported that Slackia isolated from chicken intestine w as the best detoxifying agent against mycotoxin and helps improve chicken intestinal functions (Gao et al ., 2020 ). However, several other studies reported that Slackia was found to be significantly enriched in the microbiota of gastric carcinogenesis and not only caused disease progression but also gut dysbiosis (Coker et al ., 2018 ; Kharrat et al ., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Caecal microbiota could effectively increase chicken growth performance by regulating fat metabolism

Zhang

Ansari

et al. 2021

Microbial Biotechnology

View full text Add to dashboard Cite

It has been established that gut microbiota influences chicken growth performance and fat metabolism. However, whether gut microbiota affects chicken growth performance by regulating fat metabolism remains unclear. Therefore, seven-week-old chickens with high or low body weight were used in the present study. There were significant differences in body weight, breast and leg muscle indices, and cross-sectional area of muscle cells, suggesting different growth performance. The relative abundance of gut microbiota in the caecal contents at the genus level was compared by 16S rRNA gene sequencing. The results of LEfSe indicated that high body weight chickens contained Microbacterium and Sphingomonas more abundantly (P < 0.05). In contrast, low body weight chickens contained Slackia more abundantly (P < 0.05). The results of H & E, qPCR, IHC, WB and blood analysis suggested significantly different fat metabolism level in serum, liver, abdominal adipose, breast and leg muscles between high and low body weight chickens. Spearman correlation analysis revealed that fat metabolism positively correlated with the relative abundance of Microbacterium and Sphingomonas while negatively correlated with the abundance of Slackia. Furthermore, faecal microbiota transplantation was performed, which verified that transferring faecal microbiota from adult chickens with high body weight into oneday-old chickens improved growth performance and fat metabolism in liver by remodelling the gut microbiota. Overall, these results suggested that gut microbiota could affect chicken growth performance by regulating fat metabolism.

show abstract

Section: Discussionmentioning

confidence: 99%

Caecal microbiota could effectively increase chicken growth performance by regulating fat metabolism

Zhang

Ansari

et al. 2021

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Other DON-biotransformation products, including DON-glutathione conjugates and the products of glutathione degradation, such as DON-S-cysteinyl-glycine and DON-S-cysteine, have been reported in cereals. Thanks to intestinal microflora, DON could be metabolized in animals and humans but not deposited in the tissues [ 151 , 152 ].…”

Section: Biotransformation Of Mycotoxinsmentioning

confidence: 99%

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Tran

Viktorová

Ruml

2020

Toxins

View full text Add to dashboard Cite

The determination of mycotoxins content in food is not sufficient for the prediction of their potential in vivo cytotoxicity because it does not reflect their bioavailability and mutual interactions within complex matrices, which may significantly alter the toxic effects. Moreover, many mycotoxins undergo biotransformation and metabolization during the intestinal absorption process. Biotransformation is predominantly the conversion of mycotoxins meditated by cytochrome P450 and other enzymes. This should transform the toxins to nontoxic metabolites but it may possibly result in unexpectedly high toxicity. Therefore, the verification of biotransformation and bioavailability provides valuable information to correctly interpret occurrence data and biomonitoring results. Among all of the methods available, the in vitro models using monolayer formed by epithelial cells from the human colon (Caco-2 cell) have been extensively used for evaluating the permeability, bioavailability, intestinal transport, and metabolism of toxic and biologically active compounds. Here, the strengths and limitations of both in vivo and in vitro techniques used to determine bioavailability are reviewed, along with current detailed data about biotransformation of mycotoxins. Furthermore, the molecular mechanism of mycotoxin effects is also discussed regarding the disorder of intestinal barrier integrity induced by mycotoxins.

show abstract

“…Nevertheless, the pure strain PGC-3-9 was chosen for its DON-degradation capabilities in media and wheat flour under both aerobic and anaerobic conditions ( Figure 5 and Figure 6 ). All pure strains exhibiting de-epoxidation activity on trichothecenes were anaerobic bacteria used as feed additives in animal intestinal and digestive systems [ 11 , 19 , 20 , 21 , 22 ]. Our results indicate that de-epoxidation of trichothecene mycotoxins can occur under both aerobic and anaerobic conditions.…”

Section: Discussionmentioning

confidence: 99%

Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions

Shi

Yang

et al. 2020

Toxins

View full text Add to dashboard Cite

Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling these types of mycotoxins. In this study, we isolated a novel trichothecene mycotoxin-de-epoxidating bacterium, Desulfitobacterium sp. PGC-3-9, from a consortium obtained from the soil of a wheat field known for the occurrence of frequent Fusarium head blight epidemics under aerobic conditions. Along with MMYPF media, a combination of two antibiotics (sulfadiazine and trimethoprim) substantially increased the relative abundance of Desulfitobacterium species from 1.55% (aerobic) to 29.11% (aerobic) and 28.63% (anaerobic). A single colony purified strain, PGC-3-9, was isolated and a 16S rRNA sequencing analysis determined that it was Desulfitobacterium. The PGC-3-9 strain completely de-epoxidated HT-2, deoxynivalenol (DON), nivalenol and 15-acetyl deoxynivalenol, and efficiently eliminated DON in wheat grains under aerobic and anaerobic conditions. The strain PGC-3-9 exhibited high DON de-epoxidation activity at a wide range of pH (6–10) and temperature (15–50 °C) values under both conditions. This strain may be used for the development of detoxification agents in the agriculture and feed industries and the isolation of de-epoxidation enzymes.

show abstract

Dual Function of a Novel Bacterium, Slackia sp. D-G6: Detoxifying Deoxynivalenol and Producing the Natural Estrogen Analogue, Equol

Cited by 31 publications

References 50 publications

Caecal microbiota could effectively increase chicken growth performance by regulating fat metabolism

Caecal microbiota could effectively increase chicken growth performance by regulating fat metabolism

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions

Contact Info

Product

Resources

About