Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity

Singh, Rajesh Kumar; Patil, Smita; Singh, Neetu; Gupta, Shalini

doi:10.1038/s41598-017-06014-4

Cited by 30 publications

(21 citation statements)

References 48 publications

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“… Green methods: The paths for synthesis through green chemistry are eco-friendly and toxic-free. A Formation of AuNPs with sizes of 25 + 7 nm via a simple green biosynthesis was revealed using natural biomaterial egg shell membrane (ESM) [ [44] , [45] , [46] ]. So also, ref.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of gold nanoparticles (AuNPs): A review

Sani

Cao

Cui

2021

Biochemistry and Biophysics Reports

237

157

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Gold nanoparticles are a kind of nanomaterials that have received great interest in field of biomedicine due to their electrical, mechanical, thermal, chemical and optical properties. With these great potentials came the consequence of their interaction with biological tissues and molecules which presents the possibility of toxicity. This paper aims to consolidate and bring forward the studies performed that evaluate the toxicological aspect of AuNPs which were categorized into in vivo and in vitro studies. Both indicate to some extent oxidative damage to tissues and cell lines used in vivo and in vitro respectively with the liver, spleen and kidney most affected. The outcome of these review showed small controversy but however, the primary toxicity and its extent is collectively determined by the characteristics, preparations and physicochemical properties of the NPs. Some studies have shown that AuNPs are not toxic, though many other studies contradict this statement. In order to have a holistic inference, more studies are required that will focus on characterization of NPs and changes of physical properties before and after treatment with biological media. So also, they should incorporate controlled experiment which includes supernatant control Since most studies dwell on citrate or CTAB-capped AuNPs, there is the need to evaluate the toxicity and pharmacokinetics of functionalized AuNPs with their surface composition which in turn affects their toxicity. Functionalizing the NPs surface with more peculiar ligands would however help regulate and detoxify the uptake of these NPs.

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Section: Introductionmentioning

confidence: 99%

Toxicity of gold nanoparticles (AuNPs): A review

Sani

Cao

Cui

2021

Biochemistry and Biophysics Reports

237

157

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“…Preparation of AuNP-bioconjugate probes. LPS-specific sushi peptides were conjugated to the AuNPs at a 1,000:1 molar ratio using thiol chemistry and then characterized via Fourier-transform infrared (FTIR) spectroscopy (Nicolet, Protege 460) and OPA analysis as described earlier (19). The LTA-specific antibodies were conjugated to the AuNPs using a protocol reported in the literature with slight modifications to the procedure (20,21).…”

Section: Methodsmentioning

confidence: 99%

A Flowthrough Assay for Rapid Bedside Stratification of Bloodstream Bacterial Infection in Critically Ill Patients: a Pilot Study

et al. 2018

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Bacterial infections affect more than 2 million people annually. Of these, systemic infections caused by bacteria in critically ill patients may lead to life-threatening conditions such as sepsis. We have developed a point-of-care (POC) device called Septiflo that can detect and stratify the Gram status of bloodstream bacterial infections in less than 10 min from a drop of human plasma. It works on the principle of identifying pathogen-associated molecular patterns (PAMPs), such as lipopolysaccharides (LPS) and lipoteichoic acid (LTA) that are released into the bloodstream at the onset of Gram-negative and Gram-positive bacterial infections, respectively. The biomarkers are captured on a membrane without a receptor, and the Gram status specificity is conferred by the ligands attached to gold nanoparticles (AuNPs) used as signal amplification probes. The ultrasensitive colorimetric results are read by eye down to a 100-fg/ml detection limit without an instrument. No cross-interference between the PAMPs is seen during Gram stratification. Septiflo results also display better performance than commercial enzyme-linked immunosorbent assays (ELISAs). Tests performed on 60 clinical samples from patients showed a correlation accuracy of 70% against procalcitonin (PCT), an accepted surrogate biomarker for sepsis. A direct comparison with eubacterial PCR yielded up to 94% accuracy in 31 patients at a chosen cutoff level for LPS and LTA and area under the curve (AUC) values of 0.927 and 0.885, respectively, though blood culture was negative for most samples. The high sensitivity, low cost, and simple bedside utility of the assay may aid in better sepsis management apparently at the presymptomatic stage, lowering empirical therapy, medical costs, antimicrobial resistance, and mortality.

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“…Conjugating antibiotics to nanoparticles can help to meet this goal by increasing overall drug avidity, bioavailability and internalization by mammalian cells (Singh et al . ). With respect to antibacterial activity, the general consensus is that AuNP–drug conjugates exhibit greater antibacterial activity than individual nanoparticles and drugs.…”

Section: Antimicrobial Activity Of Aunpsmentioning

confidence: 97%

Antimicrobial activity and toxicity of gold nanoparticles: research progress, challenges and prospects

Chen

2018

Lett Appl Microbiol

151

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Significance and Impact of the Study: This paper discusses the recent research progress on antimicrobial activity and toxicity of gold nanoparticles and provides general insights into the field for researchers committed to this field. AbstractGold nanoparticles are emerging materials that exhibit characteristics distinct from those of traditional materials and that have promising potential for application in the fields of chemistry, physics, biology and medicine. During the past decades, numerous studies on the antimicrobial activity and toxicity of gold nanoparticles have been published. With respect to antimicrobial activity, gold nanoparticles conjugated with small molecules, such as antibiotics, drugs, vaccines and antibodies, are more efficient than individual nanoparticles and molecules. Regarding the toxicity effects, results are often unclear and conflicting because of the lack of a standard experimental method; various studies have used different approaches, administration routes and doses, and similar experiments may lead to different conclusions. To provide a systematic overview of and insight in the current knowledge for researchers committed to this filed, we discuss the recent research advances related to the antimicrobial activity and toxicity of gold nanoparticles, both in vitro and in vivo, and identify major issues that require further study.

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Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity

Cited by 30 publications

References 48 publications

Toxicity of gold nanoparticles (AuNPs): A review

Toxicity of gold nanoparticles (AuNPs): A review

A Flowthrough Assay for Rapid Bedside Stratification of Bloodstream Bacterial Infection in Critically Ill Patients: a Pilot Study

Antimicrobial activity and toxicity of gold nanoparticles: research progress, challenges and prospects

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