Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds

Abstract

Low strength and rapid biodegradability of Acellular Dermal Matrix (ADM) restrict 37 wider clinical application as rapid cell delivery platform in situ, for management of burn 38 wounds. Herein, extracted ADM was modified by dual cross-linking approach with ionic 39 crosslinking using chitosan (CTS) and covalent cross-linking using an iodine modified 2, 5-40 dihydro-2,5-dimethoxy-furan (DHF-I) cross-linker; termed as CsADM-Cl. In addition, 41 inherent growth factors and cytokines were found to be preserved in the CsADM-Cl, 42 irrespective of ionic/covalent crosslinking. CsADM-Cl demonstrated improvement in post 43 crosslinking stiffness with decreased biodegradation rate. This hybrid crosslinked hydrogel 44 supported adhesion, proliferation, and migration of human foreskin derived fibroblasts (HFCs) 45 and keratinocytes (HKC). Also, the angiogenic potential of CsADM-Cl was manifested by 46 chick chorioallantoic membrane (CAM) assay. CsADM-Cl showed excellent antibacterial 47 activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. 48 aureus). Moreover, CsADM-Cl treated full thickness (FT) burn wounds demonstrated rapid 49 healing marked with superior angiogenesis, well-defined dermal-epidermal junctions (DEJ), 50 mature basket weave collagen deposition, and development of more pronounced secondary 51 appendages. Altogether, the bioactive CsADM-Cl hydrogel established significant clinical 52 potential to support wound healing as an apt injectable antibacterial matrix to encounter unmet 53 challenges concerning critical burn wounds.

Poly(N-vinyl imidazole) Cross-Linked β-Cyclodextrin Hydrogel for Rapid Hemostasis in Severe Renal Arterial Hemorrhagic Model

“…The in vitro antioxidant efficiency of cl -β-CD/pVIz was evaluated by measuring the ability of cl -β-CD/pVIz hydrogel to scavenge DPPH free radical. , For details, please see Supporting Information.…”

“…The hemocompatibility of the cl -β-CD/pVIz hydrogel sample was assessed using ethylenediaminetetraacetic acid (EDTA) mixed blood following a standard protocol as reported previously. ,, Briefly 5 mL of whole blood was centrifuged at 2000 rpm for 5 min to separate the red blood cells (RBCs). The RBCs were resuspended in 1× sterile PBS to perform the experiments.…”

Biomimetic Hydrogels to Promote Wound Healing

“…The hydrogel was able to speed deep partial thickness burn wound healing and resolve pains during wound dressing changes with glycine. Dhara et al ( Bankoti et al, 2020b ) constructed injectable hydrogel through blending extracted acellular dermal matrix from full thickness skin with chitosan. This hydrogel preserved innate biological composition, ultrastructure with enhanced mechanical properties and demonstrated rapid full thickness burn wound healing.…”

Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns

“…Various non-antibiotic antibacterial compounds have been used to formulate scaffolds for cutaneous tissue regeneration. Under-development products such as cellulose-based biomaterial with mineral antimicrobials such as montmorillonite nanocomposites [28], chitosan-based antibacterial scaffolds [29][30][31] and burn dressings incorporating metallic antimicrobials such as silver nanoparticles [32][33][34] or other metallic antibacterial agents such as gallium ions [35] have shown promise in promoting wound healing while exhibiting antimicrobial properties.…”