Dual gradient macroporous resin column chromatography for concurrent separation and purification of three families of marine bacterial lipopeptides from cell free broth

Dhanarajan, Gunaseelan; Rangarajan, Vivek; Sen, Ramkrishna

doi:10.1016/j.seppur.2015.01.025

Cited by 21 publications

(14 citation statements)

References 24 publications

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“…The method of affinity extraction using Macroporous adsorption resin (MARs) was used to isolate bioactive agents from cell-free supernatant (Dhanarajan et al 2015 ), MARs such as Amberlite XAD-16 and Diaion HP-20 resins were purchased from Sigma Aldrich with physical properties as listed in Table S1, resin-treated according to manufacturer instructions. Prior to use resin was soaked in absolute methanol and then washed with water and dried at 40 °C.…”

Section: Methodsmentioning

confidence: 99%

Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK

2022

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Bacillus velezensis SK having broad-spectrum antimicrobial activity has been isolated from soil. The efficient extraction of antimicrobial compounds produced in various mediums has been done using Diaion HP-20 resin. Further, characterization of an antimicrobial compound by TLC, FTIR, in-situ bioautography analysis revealed the presence of cyclic lipopeptides, which is then purified by the combination of silica gel, size exclusion, dual gradient, and RP-HPLC chromatography techniques. Growth kinetic studies showed that Bacillus velezensis SK produces a mixture of lipopeptides (1.33 gL−1). The lipopeptide exhibits good pH (2–10) and temperature stability up to 80 °C. LC–ESI–MS analysis of partially purified lipopeptide identified variant of surfactin, further analysis of purified chromatographic fractions revealed the occurrence of most abundant C15-surfactin homologues (m/z 1036.72 Da). The isolated surfactin exhibits good antimicrobial activity (1600 AU/ml) against drug-resistant food-born B. cereus and human pathogen Staphylococcus aureus. Hence, identified strain B. velezensis SK and its potent antibacterial surfactin lipopeptide could be used in various food and biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK

2022

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“…Liu et al have presented an integrated purification process based on the adsorption of surfactin on active carbon and have highlighted the strong impact of the temperature on this process. Chromatographic derivated methods were also investigated using a dual‐gradient macro‐porous resins and solvent composition changing, these methods led to an increase in the separation of the three families of lipopeptides and in the purity obtained (68% for iturin, 77% for fengycin and 91% for surfactin) .…”

Section: Purification Processes Of the Lipopeptidesmentioning

confidence: 99%

Microbial lipopeptide production and purification bioprocesses, current progress and future challenges

Coutte

Lecouturier

Dimitrov

et al. 2017

Biotechnology Journal

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Lipopeoptides are amphiphilic compounds combining interesting physicochemical properties and biological activities. Due to their high foaming capacity in aerated bioreactor, the development of scalable bioprocesses for their production is a major bottleneck. In addition, the genes involved in the biosynthesis of these lipopeptides are mainly regulated by the quorum sensing, a global regulatory mechanism depending on cell density and known to be activated in biofilms. Several approaches have thus been considered in literature taking into account two criteria, on one side, to favor, control or avoid foam formation and on the other side, to use planktonic or immobilized (biofilm) cells. These different bioprocesses are discussed in the present review along with the purification strategies proposed for extracting and concentrating these biosurfactants.

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“…In addition, the resin is easy to regenerate and the operational cost is low. In recent years, resins have been widely used for the separation of natural compounds, such as flavonoids , phenolic compounds , terpenoids , lipopeptides , and so on.…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of phloridzin from apple leaves using macroporous resins followed by preparative high‐performance liquid chromatography

Hou

et al. 2018

J of Separation Science

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Phloridzin is one of the major phenolic compounds in apple and has been widely used in medicine for a long time due to its significant biomedical activities. In this article, macroporous resin was used for purification of phloridzin from apple leaves. The HPD-300 resin was selected for the enrichement of phloridzin according to its high adsorption and desorption capacities. The adsorption kinetics and isotherms were constructed on the HPD-300 resin and fitted well to the pseudo-second-order kinetic model and Langmuir model, respectively. Dynamic adsorption and desorption tests were performed on the column packed with HPD-300 resin to optimize the operating parameters. After one round treatment with HPD-300 resin, the purity of phloridzin in the product increased from 11.4 to 50.1% with a recovery yield of 79.3%. Subsequently, preparative high-performance liquid chromatography was employed for the purification of phloridzin. The purity of phloridzin could reach above 98% after further recrystallization with a recovery yield of 75.8%.

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Dual gradient macroporous resin column chromatography for concurrent separation and purification of three families of marine bacterial lipopeptides from cell free broth

Cited by 21 publications

References 24 publications

Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK

Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK

Microbial lipopeptide production and purification bioprocesses, current progress and future challenges

Preparative separation of phloridzin from apple leaves using macroporous resins followed by preparative high‐performance liquid chromatography

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