Dual mode fibre bundle confocal endomicroscopy: combining reflectance and fluorescence imaging

Hughes, Michael; Simaiaki, Vasiliki; Chang, Tou Pin; Yang, Guang‐Zhong

doi:10.1117/12.2032551

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…To simulate the transmission through the fiber bundle, a simulator developed at the University of Kent was employed. 30 The simulated CFBs have a diameter of 350 microns and 1000 to 10000 cores. The core diameter ranges from 5.4 microns with a core-to-core distance of 9.8 microns (1000 cores) to 1.7 microns with a core-to-core distance of 3.1 microns (10000 cores).…”

Section: Simulation and Training Setupmentioning

confidence: 99%

Step-by-step transfer function reversal for single-shot 3D fiber endoscopy using a diffuser

Glosemeyer

Lich

Kuschmierz

et al. 2023

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX

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Endoscopy through coherent fiber bundles plays a significant role in industrial and medical 2D imaging. By replacing the lens on the distal side with a diffuser, the 3D information of the measurement volume is encoded as 2D speckle patterns on the camera. Neural Networks can then be employed to reconstruct the 3D object. Therefore, minimally invasive single-shot 3D imaging through a flexible low-cost endoscope with a diameter of less than 1 mm is enabled. However, the number of fiber cores is limiting the transferable information and reduces the reconstruction quality. In this paper, separate reconstruction for the diffuser and the coherent fiber bundle for different core numbers is explored. This approach enables biomedical applications for in vivo diagnostics, e.g. with fluorescence imaging in 3D.

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Section: Simulation and Training Setupmentioning

confidence: 99%

Step-by-step transfer function reversal for single-shot 3D fiber endoscopy using a diffuser

Glosemeyer

Lich

Kuschmierz

et al. 2023

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX

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“…In order to overcome the limitations of RCM, multimodal techniques have been developed to provide additional contrasts or information together with reflection contrast [8][9][10][11][12][13][14][15][16][17]. RCM was combined with fluorescence confocal microscopy (FCM) for simultaneous reflection and fluorescence imaging [8][9][10][11]. However, the RCM and FCM combination is usually not applicable to human due to the toxicity of exogenous labeling agents used in FCM, unless FCM imaging is based on tissue auto-fluorescence (AF) or FDA approved contrast agents such as fluorescein and indocyanine green (ICG).…”

Section: Introductionmentioning

confidence: 99%

High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

Kim¹,

Hoan²,

Oh³

et al. 2020

Biomed. Opt. Express

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Reflectance confocal microscopy (RCM) is a non-invasive high-resolution optical imaging technique used in clinical settings as a diagnostic method. However, RCM has limited diagnostic ability by providing non-specific morphological information only based on reflection contrast. Various multimodal imaging techniques have been developed to compensate the limitations of RCM, but multimodal techniques are often slow in imaging speed compared to RCM alone. In this report, we combined RCM with moxifloxacin based two-photon microscopy (TPM) for high-speed multimodal imaging. Moxifloxacin based TPM used clinically compatible moxifloxacin for cell labeling and could do non-invasive cellular imaging at 30 frames/s together with RCM. Performance of the combined microscopy was characterized in the imaging of mouse skin and cornea, in vivo. Detail tissue microstructures including cells, extra-cellular matrix (ECM), and vasculature were visualized. The combined microscopy was applied to human skin cancer specimens, and both cells and ECM in the skin cancer and normal skin regions were visualized at high imaging speeds. The combined microscopy can be useful in the clinical applications of RCM by providing multiple contrasts.

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Dual mode fibre bundle confocal endomicroscopy: combining reflectance and fluorescence imaging

Cited by 2 publications

References 9 publications

Step-by-step transfer function reversal for single-shot 3D fiber endoscopy using a diffuser

Step-by-step transfer function reversal for single-shot 3D fiber endoscopy using a diffuser

High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

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