Dual mode fluorometric and colorimetric detection of matrix metalloproteinase MMP-9 as a cancer biomarker based on AuNPs@gelatin/AuNCs nanocomposite

Dadmehr, Mehdi; Mortezaei, Monireh; Korouzhdehi, Behnaz

doi:10.1016/j.bios.2022.114889

Cited by 61 publications

(14 citation statements)

References 42 publications

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“…The typical approach of colorimetric assays involves the preparation of a stable colloidal solution/substrate, whose stability is intervened with by the addition of the analyte resulting in a color change. The majority of the reported studies on colorimetric analysis for biosensing use pre-formed AuNPs [ 24 , 28 , 29 , 30 , 31 ], while the C -Col Au r technique is based on the in situ formation of AuNPs in the presence of clinical samples (cervico-vaginal fluids) collected from both healthy and cancer-affected subjects. C -Col Au r is a colorimetric strategy relying on the in situ formation of AuNPs that vary in size and color depending upon the type of clinical sample leading to cervical cancer screening [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

et al. 2023

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This paper reports the colorimetric analysis of cervical-cancer-affected clinical samples by the in situ formation of gold nanoparticles (AuNPs) formed with cervico-vaginal fluids collected from healthy and cancer-affected patients in a clinical setup, termed “C-ColAur”. We evaluated the efficacy of the colorimetric technique against the clinical analysis (biopsy/Pap smear) and reported the sensitivity and specificity. We investigated if the aggregation coefficient and size of the nanoparticles responsible for the change in color of the AuNPs (formed with clinical samples) could also be used as a measure of detecting malignancy. We estimated the protein and lipid concentrations in the clinical samples and attempted to investigate if either of these components was solely responsible for the color change, enabling their colorimetric detection. We also propose a self-sampling device, CerviSelf, that could enable the rapid frequency of screening. We discuss two of the designs in detail and demonstrate the 3D-printed prototypes. These devices, in conjugation with the colorimetric technique C-ColAur, have the potential to be self-screening techniques, enabling women to undergo rapid and frequent screening in the comfort and privacy of their homes, allowing a chance at an early diagnosis and improved survival rates.

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Section: Resultsmentioning

confidence: 99%

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

et al. 2023

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“…The as-prepared sensor was successfully applied to serum samples, with recovery percentages of 95%−108%. 126 5.5. Enzyme Activity Assays.…”

Section: Macromoleculesmentioning

confidence: 99%

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Mohseni,

Moodi,

Kefayat

et al. 2024

ACS Omega

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Development of rapid colorimetric methods based on novel opticalactive metal nanomaterials has provided methods for the detection of ions, biomarkers, cancers, etc. Fluorescent metal nanoclusters (FMNCs) have gained a lot of attention due to their unique physical, chemical, and optical properties providing numerous applications from rapid and sensitive detection to cellular imaging. However, because of very small color changes, their colorimetric applications for developing rapid tests based on the naked eye or simple UV−vis absorption spectrophotometry are still limited. FMNCs with peroxidase-like activity have significant potential in a wide variety of applications, especially for point-of-care diagnostics. In this review, the effect of using various capping agents and metals for the preparation of nanoclusters in their colorimetric sensing properties is explored, and the synthesis and detection mechanisms and the recent advances in their application for ultrasensitive chemical and biological analysis regarding human health are highlighted. Finally, the challenges that remain as well as the future perspectives are briefly discussed. Overcoming these limitations will allow us to expand the nanocluster's application for colorimetric diagnostic purposes in medical practice.

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“…In addition to environmental heavy metal detection, AuNPs can also be used to analyze cancer biomarkers through dual-mode fluorescence colorimetry. Dadmehr [48] et al used AuNPs grafted with gelatin and combined with gold nanoclusters to detect matrix metalloproteinase. Food samples can also be detected by AuNPs colorimetric method.…”

Section: Fluorescent and Colorimetric Assaymentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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Molecular biology applications based on gold nanotechnology have revolutionary impacts, especially in diagnosing and treating molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has increased the detection of molecules and the possibility of atoms. These advantages have brought medical research to the cellular level for application potential. Many research groups are working towards this. The superior analytical properties of gold nanoparticles can not only be used as an effective drug screening instrument for gene sequencing in new drug development but also as an essential tool for detecting physiological functions, such as blood glucose, antigen-antibody analysis, etc. The review introduces the principles of biomedical sensing systems, the principles of nanomaterial analysis applied to biomedicine at home and abroad, and the chemical surface modification of various gold nanoparticles.

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Dual mode fluorometric and colorimetric detection of matrix metalloproteinase MMP-9 as a cancer biomarker based on AuNPs@gelatin/AuNCs nanocomposite

Cited by 61 publications

References 42 publications

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

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