Dual Protection: More Needed than Practised or Understood

Berer, Marge

doi:10.1016/s0968-8080(06)28262-4

Cited by 50 publications

(48 citation statements)

References 33 publications

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“…Samples were incubated at 37°C and constantly shaken. At fixed time points (1,2,4,8,24,48, and 72 h and 1, 2, 3, and 4 weeks), 1 ml of SVF from each sample was collected and replaced with another 1 ml of fresh SVF. The quantity of GRFT released from the fiber surface in the SVF was quantified by ELISA.…”

Section: Methodsmentioning

confidence: 99%

“…Human immunodeficiency virus type 1 (HIV-1) affects ϳ35 million people globally (2)(3)(4)(5), while untreated STIs, such as those caused by herpes simplex virus 2 (HSV-2), can enhance both the acquisition and the transmission of HIV and other agents of STIs by 2-to 4-fold (6,7). In light of the findings of recent clinical trials, a specific, multipurpose prevention technology that has the ability to prevent multiple STIs using one delivery platform and that also increases user adherence urgently needs to be developed (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)). In the study described here, we sought to shift current topical preexposure prophylaxis (PrEP) paradigms by integrating a multipurpose biological delivery approach to debilitate and inactivate HIV.…”

mentioning

confidence: 99%

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Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Grooms

Vuong

Tyo

et al. 2016

Antimicrob Agents Chemother

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Despite current prophylactic strategies, sexually transmitted infections (STIs) remain significant contributors to global health challenges, spurring the development of new multipurpose delivery technologies to protect individuals from and treat virus infections. However, there are few methods currently available to prevent and no method to date that cures human immunodeficiency virus (HIV) infection or combinations of STIs. While current oral and topical preexposure prophylaxes have protected against HIV infection, they have primarily relied on antiretrovirals (ARVs) to inhibit infection. Yet continued challenges with ARVs include user adherence to daily treatment regimens and the potential toxicity and antiviral resistance associated with chronic use. The integration of new biological agents may avert some of these adverse effects while also providing new mechanisms to prevent infection. Of the biologic-based antivirals, griffithsin (GRFT) has demonstrated potent inhibition of HIV-1 (and a multitude of other viruses) by adhering to and inactivating HIV-1 immediately upon contact. In parallel with the development of GRFT, electrospun fibers (EFs) have emerged as a promising platform for the delivery of agents active against HIV infection. In the study described here, our goal was to extend the mechanistic diversity of active agents and electrospun fibers by incorporating the biologic GRFT on the EF surface rather than within the EFs to inactivate HIV prior to cellular entry. We fabricated and characterized GRFT-modified EFs (GRFT-EFs) with different surface modification densities of GRFT and demonstrated their safety and efficacy against HIV-1 infection in vitro. We believe that EFs are a unique platform that may be enhanced by incorporation of additional antiviral agents to prevent STIs via multiple mechanisms. N ewly acquired sexually transmitted infections (STIs) affect 340 million people per year (1-3) and exert a significant impact on global health. Human immunodeficiency virus type 1 (HIV-1) affects ϳ35 million people globally (2-5), while untreated STIs, such as those caused by herpes simplex virus 2 (HSV-2), can enhance both the acquisition and the transmission of HIV and other agents of STIs by 2-to 4-fold (6, 7). In light of the findings of recent clinical trials, a specific, multipurpose prevention technology that has the ability to prevent multiple STIs using one delivery platform and that also increases user adherence urgently needs to be developed (8-18). In the study described here, we sought to shift current topical preexposure prophylaxis (PrEP) paradigms by integrating a multipurpose biological delivery approach to debilitate and inactivate HIV.Our long-term goal is to develop a multipurpose biologically inspired electrospun fiber (EF) prevention technology that takes cues from the innate microenvironment of the female reproductive tract to more strategically narrow the gaps in microbicide efficacy. In this work, we evaluated the potential of polymeric EF scaffolds surface modified with the po...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Grooms

Vuong

Tyo

et al. 2016

Antimicrob Agents Chemother

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show abstract

“…Despite the many forms of DP, their practice and effectiveness are variable. For example, complete abstinence is the most effective DP practice; however, 'not many [youths] are willing to stop having sex altogether' [24]. Moreover, uninfected infertile couples engaging in unprotected sex in a monogamous relationship would constitute DP; but this is unlikely for many youths as these age-groups have high fecundity, form fragile relationships, and often engage in casual sex [25,26].…”

Section: Introductionmentioning

confidence: 99%

Correlates and Barriers of Dual-Method Contraception among College Youths in Nigeria

Buraimo¹,

Simkhada²,

Watson³

et al. 2017

J Contracept Stud

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Background and purpose: The dual problems of unintended pregnancies and sexually transmitted infections/Human Immunodeficiency virus together represent one of the most significant public health challenges globally, particularly amongst youths in Sub-Saharan Africa. Although the simultaneous use of two contraceptives, dual method contraception (DMC), is highly effective in preventing these problems, our understanding of the use of DMC is limited in developing country contexts. Thus, this study sets out to assess the correlates and barriers of DMC use amongst youths in Nigeria.

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“…While the primary aim of contraception is to prevent unplanned pregnancies, two barrier contraceptives methods i.e. male and female condoms can also prevent Sexually Transmitted Infections (STIs), HIV inclusive [2,3].…”

Section: Introductionmentioning

confidence: 99%

Sexual Health Behaviours: HIV/STI Risk Perception among Namibian University Students

Olawale¹,

Ifeanyi²

2016

Fam Med Med Sci Res

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Background: The attitude of young people towards the use of contraceptives differs and it is linked with their sexuality. One of the most frequent assertions for the lack of use of the condoms as contraceptive is that boys did not like using them because it reduces the feeling and pleasure during intercourse and some of the young mothers' belief that putting on condom just prior to intercourse did interrupt the spontaneous feelings during sexual intimacy

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Dual Protection: More Needed than Practised or Understood

Cited by 50 publications

References 33 publications

Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Correlates and Barriers of Dual-Method Contraception among College Youths in Nigeria

Sexual Health Behaviours: HIV/STI Risk Perception among Namibian University Students

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