Dual Sensitization Smartphone Colorimetric Strategy Based on RCA Coils Gathering Au Tetrahedra and Its Application in the Detection of CK-MB

Abstract

In recent years, the combination of DNA nanotechnology and biosensing has been extensively reported. Herein, we attempted to develop a dual sensitization smartphone colorimetric strategy based on rolling circle amplification (RCA) coils gathering Au tetrahedra and explore its application. The dual sensitization effect of this strategy was achieved by rolling circle amplification (RCA) and Au tetrahedra. Under the initiation of the complementary DNA, a large number of ssDNA were generated, achieving amplification of the reaction signal. At the same time, due to the formation of Au tetrahedra, more gold nanoparticles could be gathered under the same conditions, and the signal would be amplified again. Using software ImageJ, the gray value of the reaction solution can be analyzed, detecting the target timely under the practical conditions of lack of equipment. By selecting aptamers with strong binding affinity, we applied this strategy to detect creatine kinase isoenzymes (CK-MB), showing a limit of detection of 0.8 pM, which performed well in actual detection and can meet the needs for real-time detection of CK-MB. Therefore, a universal detection platform was developed, which has broad application prospects in biosensing, clinical diagnosis, food detection, and other fields.

Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis

“…By selecting aptamers with strong binding affinity, this method achieved a detection limit of 0.8 pM for creatine kinase isoenzyme detection. The amplification strategy using rolling circle amplification was successfully used for the detection of creatine kinase isoenzymes, demonstrating its potential for application in food safety analysis (Figure E) …”

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

“…Consequently, the smartphone-based aptasensor exhibited a LOD of 264 pM in spiked buffer and 352 pM in spiked serum. For the CK-MB, Zhang et al [ 93 ] proposed a dual sensitization smartphone colorimetric strategy to detect CK-MB gathering rolling circle amplification (RCA) coils and Au tetrahedra. The platform demonstrated reasonable specificity with a low LOD of 0.8 pM and performed well in spiked human serum samples.…”

Self-Priming DNA Polymerization-Propelled Stochastic Walkers on Magnetic Microbeads for Amplified Detection of miRNA

“…Next-generation sequencing (NGS) technology is also a powerful tool to quantify and analyze RNA targets. , Nevertheless, the demand for sequencing length and sample preparation still hinders its widespread usage. In recent years, a lot of nonenzymatic or enzymatic nucleic acid amplification technologies including strand displacement amplification (SDA), − rolling circle amplification (RCA), , loop-mediated amplification (LAMP), , hybridization chain reaction (HCR), − and catalytic-hairpin assembly (CHA) , have been reported to detect miRNAs in solutions. Some of these strategies can even achieve high sensitivity comparable to that of the PCR.…”