“Dual Signal-On” Split-Type Aptasensor for TNF-α: Integrating MQDs/ZIF-8@ZnO NR Arrays with MB-Liposome-Mediated Signal Amplification

Abstract

Ultrasensitive and accurate detection of biomarkers in serum is of great importance for disease diagnosis and treatment. So far, the commonly used single-mode signal suffers from certain instinct drawbacks that restrict assay performances. Herein, we report the proof-of-concept fabrication of a split-type photoelectrochemical (PEC) and electrochemical (EC) dual-modal aptasensor for ultrasensitively tracing tumor necrosis factor-α, a noteworthy biological biomarker with essential clinical importance. By smart integrating molybdenum disulfide QDs/zeolitic imidazolate framework-8@ZnO nanorod arrays with a methylene blue-liposome-mediated signal amplification strategy, “dual signal-on” detection is accomplished based on a sandwich reaction of the target with aptamer-anchored carboxyl magnetic beads and an aptamer-confined MB liposome. Linear ranges of 5 fg/mL–5 μg/mL (detection limit 1.46 fg/mL) for PEC and 10 fg/mL–0.5 μg/mL (detection limit 6.14 fg/mL) for EC are obtained, respectively. An independent signal transduction mechanism supports the accuracy improvement, and a separate biological process from a translator enables convenient fabrication, short-time consumption, wider linearity, as well as outstanding reproducibility and stability in practical application. This work presents a universal bioassay route with prospects in biomedical and related areas.

Liposomal Controlled Release Ag-Activated DNAzyme Cycle Amplification on a 2D Pyrene COF-Based Photocathode for α-Synuclein Immunosensing

“…The Ag NPs were synthesized according to the previous literature using sodium borohydride reduction of AgNO 3 . Ag NPs-loaded liposomes (ALLs) were prepared through a thin lipid film hydration method according to our previous work . Details can be found in the Supporting Information.…”

“…Ag NPsloaded liposomes (ALLs) were prepared through a thin lipid film hydration method according to our previous work. 44 Details can be found in the Supporting Information.…”

Signal-on photoelectrochemical immunoassay for salivary cortisol based on silver nanoclusters-triggered ion-exchange reaction with CdS quantum dots

“…It is well-known that signal-on biosensors are superior to signal-off ones because of their lower background signal and probability for false-positive results [ 12 , 13 ]. What’s more, in PEC immunoassay, the quantitative analysis of biomarkers is usually carried out through photocurrent changes after biological recognition.…”

Highly Selective and Sensitive microRNA-210 Assay Based on Dual-Signaling Electrochemical and Photocurrent-Polarity-Switching Strategies

“…10,11 However, the quantitation analysis depending upon a single-mode readout signal may be affected by possible co-existed interferents, different operating personnels, instruments, and nonstandard analysis processes. 12 In order to resolve the abovementioned issues, dual-mode biosensing methods have already been developed recently for miRNA assay based on two types of response mechanisms and comparatively distinct signaling transductions, such as EC− PEC, 13,14 PEC−colorimetry (CL), 15,16 SERS−EC, 17 SERS− FL, 18,19 FL−CL, 20 and circular dichroism−luminescence. 21 The dual-mode biosensing strategy not only has unique properties of each mode but also offers reciprocal authentication of detection results in various modes, effectively increasing the accuracy and confidence level of assay.…”

“…In order to resolve the abovementioned issues, dual-mode biosensing methods have already been developed recently for miRNA assay based on two types of response mechanisms and comparatively distinct signaling transductions, such as EC–PEC, , PEC–colorimetry (CL), , SERS–EC, SERS–FL, , FL–CL, and circular dichroism–luminescence . The dual-mode biosensing strategy not only has unique properties of each mode but also offers reciprocal authentication of detection results in various modes, effectively increasing the accuracy and confidence level of assay. , In these dual-mode biosensing methods that have been reported, EC–PEC dual-mode analysis has attracted widespread concern owing to its easy portability, low cost, fast response, and so forth. , Moreover, the EC–PEC analysis only demands one electrochemical apparatus but possesses two types of readout signals, which makes the biosensing platform have the advantage of a simple detecting structure.…”