Dual specificity phosphatase 6 suppresses the growth and metastasis of prostate cancer cells

Zhai, Xiao-Lei; Han, Qian; Zhang, Shan; Qu, Xiaowei; Guo, Liang; Zhou, Yifeng

doi:10.3892/mmr.2014.2575

Cited by 13 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prostate cancer is a rather common disease threatening the health of older males [ 30 ]. Global statistics show that, in 2008, the standardized morbidity and mortality rates of this cancer were 82.5/100,000 and 7.5/100,000, respectively [ 31 – 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Global statistics show that, in 2008, the standardized morbidity and mortality rates of this cancer were 82.5/100,000 and 7.5/100,000, respectively [ 31 – 33 ]. The incidence of the disease was relatively low in China previously, but in recent years we have seen an increase in its incidence and mortality [ 30 ]. At present, no effective treatment method has been introduced for advanced-stage prostate cancer.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Association betweenHIF1Ars11549465 polymorphism and risk of prostate cancer: a meta-analysis

Fang

et al. 2017

Oncotarget

View full text Add to dashboard Cite

The hypoxia inducible factor 1-alpha (HIF1A) gene has been suggested to play a critical role in cancer progression, and the relationship between HIF1A rs11549465 polymorphism and risk of prostate cancer has been investigated in previous studies. Nevertheless, conflicting results have been obtained. Hence, we reevaluated this issue by means of this meta-analysis, with the purpose of providing more precise conclusion on this issue. The electronic databases of PubMed, EMBASE and Chinese National Knowledge Infrastructure (CNKI) as well as other sources were searched for relevant reports concerning on the role of HIF1A rs11549465 polymorphism in the occurrence of prostate cancer. The strength of the relationship was determined by calculating odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs). Besides, subgroup analyses by ethnicity and source of control were further performed to examine this relationship. All statistical analyses were performed using STATA software 12.0. Although HIF1A rs11549465 polymorphism showed a tendency of increasing the risk of prostate cancer, no statistical significance was detected under any genetic models. Similar results were also revealed in subgroup analyses on the basis of ethnicity and control source. Our findings indicate that HIF1A rs11549465 polymorphism may not independently play a significant role in the occurrence of prostate cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association betweenHIF1Ars11549465 polymorphism and risk of prostate cancer: a meta-analysis

Fang

et al. 2017

Oncotarget

View full text Add to dashboard Cite

show abstract

“…At current, 38 DUSP family members are known, and 11 use MAP kinases as substrate; of those, only DUSP6 displays high selectivity for ERK1/2, the others are promiscuity towards JNK, p38 or ERK [ 15 ]. Dusp6 is expressed in multiple tissues, with highest expression in the brain [ 16 ], adipose tissue [ 17 ], heart and pancreas [ 15 ] and has been implicated in multiple physiological processes ranging from brain development [ 18 ] and heart function [ 19 ] to tumorigenesis [ 20 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Dual specificity phosphatase 6 deficiency is associated with impaired systemic glucose tolerance and reversible weight retardation in mice

et al. 2017

View full text Add to dashboard Cite

Here, we aimed to investigate the potential role of DUSP6, a dual specificity phosphatase, that specifically inactivates extracellular signal-regulated kinase (ERK), for the regulation of body weight and glucose homeostasis. We further assessed whether metabolic challenges affect Dusp6 expression in selected brain areas or white adipose tissue. Hypothalamic Dusp6 mRNA levels remained unchanged in chow-fed lean vs. high fat diet (HFD) fed obese C57Bl/6J mice, and in C57Bl/6J mice undergoing prolonged fasting or refeeding with fat free diet (FFD) or HFD. Similarly, Dusp6 expression levels were unchanged in selected brain regions of Lepob mice treated with 1 mg/kg of leptin for 6 days, compared to pair-fed or saline-treated Lepob controls. Dusp6 expression levels remained unaltered in vitro in primary adipocytes undergoing differentiation, but were increased in eWAT of HFD-fed obese C57Bl/6J mice, compared to chow-fed lean controls. Global chow-fed DUSP6 KO mice displayed reduced body weight and lean mass and slightly increased fat mass at a young age, which is indicative for early-age weight retardation. Subsequent exposure to HFD led to a significant increase in lean mass and body weight in DUSP6 deficient mice, compared to WT controls. Nevertheless, after 26 weeks of high-fat diet exposure, we observed comparable body weight, fat and lean mass in DUSP6 WT and KO mice, suggesting overall normal susceptibility to develop obesity. In line with the increased weight gain to compensate for early-age weight retardation, HFD-fed DUSP6 KO displayed increased expression levels of anabolic genes involved in lipid and cholesterol metabolism in the epididymal white adipose tissue (eWAT), compared to WT controls. Glucose tolerance was perturbed in both chow-fed lean or HFD-fed obese DUSP6 KO, compared to their respective WT controls. Overall, our data indicate that DUSP6 deficiency has limited impact on the regulation of energy metabolism, but impairs systemic glucose tolerance. Our data are in conflict to earlier reports that propose protection from diet-induced obesity and glucose intolerance in DUSP6 deficient mice. Reasons for the discrepancies remain elusive, but may entail differential genetic backgrounds, environmental factors such as the type and source of HFD, or alterations in the gut microbiome between facilities.

show abstract

“…Prostate cancer is a disease that affects older males, and is one of the most common malignant tumors amongst males in Europe (1). Previously, prostate cancer was observed relatively rarely in China; however, in recent years the incidence of prostate cancer and associated mortality rates have been increasing (1).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, prostate cancer was observed relatively rarely in China; however, in recent years the incidence of prostate cancer and associated mortality rates have been increasing (1).…”

Section: Introductionmentioning

confidence: 99%

Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182

Li¹,

Ma²,

Yang³

2015

Oncology Letters

View full text Add to dashboard Cite

Abstract. Mangiferin, a flavonoid extracted from the mango tree, possesses anti-inflammatory, antibacterial, anti-herpes simplex and antitumor activity, and is able to affect immune function. The present study investigated the anticancer effects of mangiferin treatment on PC3 human prostate cancer cells, and the potential underlying mechanisms. In the present study, an MTT assay was used to analyze the proliferation of PC3 cells. Subsequently, flow cytometry and colorimetric assay kits were utilized to measure the PC3 cell apoptotic rate. The expression levels of B-cell lymphoma-2 (Bcl-2) and microRNA-182 (miR-182) were detected using western blot analysis and quantitative reverse transcription-polymerase chain reaction, respectively. Finally, miR-182 and anti-miR-182 were transfected into PC3 cells, which were used to investigate the effects of mangiferin. Mangiferin treatment reduced the proliferation of PC3 human prostate cancer cells in a concentration-and time-dependent manner. In addition, mangiferin was able to promote apoptosis and induce the caspase-3 activity of PC3 human prostate cancer cells. Mangiferin treatment was also able to significantly reduce Bcl-2 expression levels and enhance miR-182 expression in PC3 cells. Finally, it was observed that mangiferin inhibited proliferation and induced apoptosis in PC3 human prostate cancer cells, and this effect was correlated with downregulation of Bcl-2 and upregulation of miR-182. IntroductionProstate cancer is a disease that affects older males, and is one of the most common malignant tumors amongst males in Europe (1). Previously, prostate cancer was observed relatively rarely in China; however, in recent years the incidence of prostate cancer and associated mortality rates have been increasing (1).In 2008, the worldwide standardized incidence rate of prostate cancer was 28.5/100,000 and the standardized mortality rate was 7.5/100,000 (2-4). Therefore, prostate cancer has become a significant area of research.Cell apoptosis may be inseparable from the development of prostate cancer. Apoptosis, or programmed cell death, differs from necrosis, the natural cell death process (5). Reduced apoptosis and increased proliferation are thought to be the primary mechanisms underlying the formation of the majority of tumors. Inhibition of apoptosis may also possess a significant role in the progression of prostate cancer, and B-cell lymphoma-2 (Bcl-2) is thought to be one of the most significant genes required for apoptosis (6).MicroRNAs (miRs) are a class of endogenously expressed non-protein encoding RNAs (2). Mature miRs are single-stranded and consist of ~22 nucleotides (7). Previous studies have indicated that miRs may be involved in human tumorigenesis and development. Numerous tumors exhibit alterations in miR expression levels, and these miRs may have a role in tumorigenesis, functioning as cancer genes or tumor suppressor genes (8). Casanova-Salas et al (9) demonstrated that miR-182 was a potential biomarker for prostate cancer diagnosis, prognosis predict...

show abstract

Dual specificity phosphatase 6 suppresses the growth and metastasis of prostate cancer cells

Cited by 13 publications

References 31 publications

Association betweenHIF1Ars11549465 polymorphism and risk of prostate cancer: a meta-analysis

Association betweenHIF1Ars11549465 polymorphism and risk of prostate cancer: a meta-analysis

Dual specificity phosphatase 6 deficiency is associated with impaired systemic glucose tolerance and reversible weight retardation in mice

Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182

Contact Info

Product

Resources

About