Duck hepatitis A virus structural proteins expressed in insect cells self-assemble into virus-like particles with strong immunogenicity in ducklings

Wang, Anping; Gu, L.L.; Wu, Shuang; Zhu, Shanyuan

doi:10.1016/j.vetmic.2017.12.020

Cited by 18 publications

(6 citation statements)

References 25 publications

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“…On the other hand, rDAdV3-VP1-188 provided protection against DHAV1, but DAdV3 and PBS groups did not, suggesting that VP1 expressed by rDAdV3-VP1-188 produced immunogenicity and provided protection. Although rDAdV3-VP1-188 does not provide 100% protection against DHAV1, which may be due to insufficient protection provided by VP1 protein alone, and also requires VP0 protein ( Wang et al, 2018 ; Niu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus

Wen,

Kong,

Shen

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus

Wen,

Kong,

Shen

et al. 2023

Poultry Science

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“…No. FJ157178) was amplified by PCR from pFB-P13CD (Wang et al, 2018) vector using primers 5'-gtactcgagaccATGGGAAAGAGA AAACCACGCAAG-3' and 5'-gtagcggccgcTTACTGATTATTG GTTGCCATCTGC-3' (Xho I and Not I sites are underlined; uppercases indicate the VP3 coding sequence), a start codon (ATG) and a stop codon (TGA) were included in the primers, and a Kozak sequence was introduced in front of the start codon to improve the efficiency of VP3 protein translation. The PCR product was cut with XhoI and NotI and cloned downstream of the CMV promoter in a previously developed vector pFBAGFP containing the inverted terminal repeats (ITRs) of AAAV (Wang et al, 2017), and the recombinant vector was designated as pFB-VP3 (Fig.…”

Section: Methodsmentioning

confidence: 99%

Expression of duck hepatitis A virus type 1 VP3 protein mediated by avian adeno-associated virus and its immunogenicity in ducklings

Wang¹,

Liu²,

Gu³

et al. 2019

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The avian adeno-associated virus (AAAV) is a replication-defective nonpathogenic virus that has been proved to be useful as a viral vector in gene delivery. In this study, the feasibility of AAAV for transgenic expression of duck hepatitis A virus (DHAV) VP3 structural protein and its ability to induce protective immunity in ducklings was assessed. The recombinant AAAV (rAAAV-VP3) expressing the VP3 protein was prepared by co-infection of Sf9 cells with recombinant baculovirus (rBac-VP3) containing VP3 gene flanked by inverted terminal repeats (ITRs) of AAAV and the other two recombinant baculovirus expressing AAAV functional and structural genes, respectively. The generation of rAAAV-VP3 was demonstrated by electron microscopy, immunofluorescence assay, and western blot analysis. One day old ducklings were inoculated with rAAAV-VP3 or commercial attenuated vaccine and then challenged with DHAV-1 strain SH two weeks post vaccination. Anti-DHAV-1 antibodies were detected in all vaccinated groups by ELISA, and the titers between the rAAAV-VP3 group and the attenuated vaccine group were not statistically significant. Real time RT-PCR analysis showed that the virus copy numbers in the livers of the PBS control group were significantly higher than that of the rAAAV-VP3 and attenuated vaccine groups. In conclusion, we demonstrated that the VP3 expression mediated by rAAAV in ducklings could induce protective immunity against DHAV challenge, and this could be a candidate vaccine for the control of duck viral hepatitis.

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“…Schematic representation of main types of nanoparticles used for vaccine production: (1) VLPs, (2) liposome-based particles [24], (3) nondegradable spherical nanoparticles (for example, metal nanoparticles) [25], (4) polymer nanoparticles [26], (5) graphene nanosheets [27] and nanotubes [28]. Antigen of the duck hepatitis A virus produced in the baculovirus expression system assembles into VLPs immediately in the cultured Spodoptera frugiperda (sf9) cells, while immunization of ducklings with the obtained VLPs induces a high level humoral immune response and protects them from developing the disease [46]. The abilities of VLPs resulting from multimerization of the cloned virus protein to play the role of an immunogen are not limited just to the presentation of their proper epitopes but may also be taken advantage of to display heterologous proteins.…”

Section: Natural Sources For Vlp Bioengineering and The Specificmentioning

confidence: 99%

Virus-Like Particles as an Instrument of Vaccine Production

Syomin

Ilyin

2019

Mol Biol

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The paper discusses the techniques which are currently implemented for vaccine production based on virus-like particles (VLPs). The factors which determine the characteristics of VLP monomers assembly are provided in detail. Analysis of the literature demonstrates that the development of the techniques of VLP production and immobilization of target antigens on their surface have led to the development of universal platforms which make it possible for virtually any known antigen to be exposed on the particle surface in a highly concentrated form. As a result, the focus of attention has shifted from the approaches to VLP production to the development of a precise interface between the organism's immune system and the peptides inducing a strong immune response to pathogens or the organism's own pathological cells. Immunome-specified methods for vaccine design and the prospects of immunoprophylaxis are discussed. Certain examples of vaccines against viral diseases and cancers are considered.

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Duck hepatitis A virus structural proteins expressed in insect cells self-assemble into virus-like particles with strong immunogenicity in ducklings

Cited by 18 publications

References 25 publications

Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus

Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus

Expression of duck hepatitis A virus type 1 VP3 protein mediated by avian adeno-associated virus and its immunogenicity in ducklings

Virus-Like Particles as an Instrument of Vaccine Production

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