Duck Viral Enteritis: Microtiter Plate Isolation and Neutralization Test Using the Duck Embryo Fibroblast Cell Line

Wolf, Ken; Burke, C. N.; Quimby, M. C.

doi:10.2307/1589110

Cited by 17 publications

(12 citation statements)

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“…Wolfs t al. [ 17], also using the Lake An des isolate, preferred CCL-141 cells for diag nostic studies because of a more basophilic staining characteristic relative to Pekin cells. The virus yield in Pekin cells was 2.6 PFU/ cell, whereas that for CCL-141 cells was only 0.6 PFU/cell.…”

Section: Discussionmentioning

confidence: 99%

Improvement in Plaquing Methods for the Enumeration of Anatid Herpesvirus (Duck Plague Virus)

Attanasio¹,

Olson²,

Johnson³

1980

Intervirology

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The Holland strain of anatid herpesvirus (AHV) was 2- to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or Nobel agar. Virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h postinfection. The AHV dose-response curve was linear. Pekin duck embryo cultures were more efficient than CCL-141 in plaque response and gave the maximum virus yield per cell. Virus titers were independent of avian cell passage number (to 6), fetal or newborn calf sera (to 10% v/v), and the presence of polyions during adsorption. The host range included cells from several species of Anseriformes and Galliformes.

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Section: Discussionmentioning

confidence: 99%

Improvement in Plaquing Methods for the Enumeration of Anatid Herpesvirus (Duck Plague Virus)

Attanasio¹,

Olson²,

Johnson³

1980

Intervirology

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“…The morphogenesis and distribution of virus in vivo can be observed by electron microscopy . VN assay can be conducted in duck/ chicken embryo, embryo fibroblasts or cell culture (Wolf et al 1974). Neutralization indices (NI) from 0 to 1.5 are considered as lack of exposure of birds to DEV, whereas NI > 1.75 is considered as evidence of prior exposure to DEV (Dardiri & Hess 1967).…”

Section: Immunological / Serological Testsmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

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“…The constant virus-diluted serum .VNT was carried out in 96-well flat bottomed plates (Wolf et al, 1974). Twofold dilutions of serum were prepared in MEM.…”

Section: Virus Neutralisation Test (Vnt)mentioning

confidence: 99%

Absence of Neutralising antibodies to duck plague virus in the commercial duck and goose populations in West Germany (1980–1985)

Toro

Kaleta

1986

Avian Pathology

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Duck Viral Enteritis: Microtiter Plate Isolation and Neutralization Test Using the Duck Embryo Fibroblast Cell Line

Cited by 17 publications

References 0 publications

Improvement in Plaquing Methods for the Enumeration of Anatid Herpesvirus (Duck Plague Virus)

Improvement in Plaquing Methods for the Enumeration of Anatid Herpesvirus (Duck Plague Virus)

Duck virus enteritis (duck plague) – a comprehensive update

Absence of Neutralising antibodies to duck plague virus in the commercial duck and goose populations in West Germany (1980–1985)

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