Dunkerque City air pollution particulate matter-induced cytotoxicity, oxidative stress and inflammation in human epithelial lung cells (L132) in culture

Garçon, Guillaume; Dagher, Zeina; Zerimech, Farid; Ledoux, Frédéric; Courcot, Dominique; Aboukaı̈s, Antoine; Puskaric, E.; Shirali, Pirouz

doi:10.1016/j.tiv.2005.09.012

Cited by 119 publications

(68 citation statements)

References 47 publications

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“…Tetraethoxypropane (Sigma-Aldrich), which hydrolyzes to yield one molecule of MDA, was used as standard, with thiobarbituric acid (TBA) as reagent. A 50-ml volume of standard solution or methanol extract was injected into the HPLC system and the MDA-TBA adducts were detected at ϭ 532 nm (18).…”

Section: Study Of Oxidative Stress Markersmentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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The age-related impairment of endothelium-dependent vasodilatation contributes to increased cardiovascular risk in the elderly. For primary and secondary prevention, aspirin can reduce the incidence of cardiovascular events in this patient population. The present work evaluated the effect of low-dose aspirin on age-related endothelial dysfunction in C57B/J6 aging mice and investigated its protective antioxidative effect. Agerelated endothelial dysfunction was assessed by the response to acetylcholine of phenylephrine-induced precontracted aortic segments isolated from 12-, 36-, 60-, and 84-wk-old mice. The effect of low-dose aspirin was examined in mice presenting a decrease in endothelial-dependent relaxation (EDR). The effects of age and aspirin treatment on structural changes were determined in mouse aortic sections. The effect of aspirin on the oxidative stress markers malondialdehyde and 8-hydroxy-2Ј-deoxyguanosine (8-OhdG) was also quantified. Compared with that of 12-wk-old mice, the EDR was significantly reduced in 60-and 84-wk-old mice (P Ͻ 0.05); 68-wkold mice treated with aspirin displayed a higher EDR compared with control mice of the same age (83.9 Ϯ 4 vs. 66.3 Ϯ 5%; P Ͻ 0.05). Aspirin treatment decreased 8-OHdG levels (P Ͻ 0.05), but no significant effect on intima/media thickness ratio was observed. The protective effect of aspirin was not observed when treatment was initiated in older mice (96 wk of age). It was found that low-dose aspirin is able to prevent age-related endothelial dysfunction in aging mice. However, the absence of this effect in the older age groups demonstrates that treatment should be initiated early on. The underlying mechanism may involve the protective effect of aspirin against oxidative stress. endothelium; intima/media; vasodilatation; oxidative stress AGE-ASSOCIATED CHANGES SUCH as endothelial dysfunction are involved in the significantly increased risk of cardiovascular complications and microthrombus formation in the elderly patient population (44). The presence of endothelial dysfunction in the coronary or peripheral circulation has been shown to constitute a risk factor for cardiovascular events independent of the development of atherosclerosis or other vascular risk factors (22,(43)(44)(45). There is emerging evidence that age-associated endothelial dysfunction is related to the local formation of reactive oxygen and nitrogen species within and in the vicinity of the vascular wall (13,25,36,43,49). Therapeutic approaches capable of preventing or reversing age-related endothelial dysfunction may thus help to reduce cardiovascular risk in the elderly.Age-related endothelial-dependent relaxation (EDR) decreases in the large vessels of different animal species including humans (36). EDR in response to acetylcholine (ACh) decreases with age in the rat aorta: the maximal relaxation effect of ACh is 100% in 4-to 6-wk-old, 50% in 3-to 6-mo-old, and 25% in 12-to 25-mo-old rats (29). The agerelated decrease in EDR varies from one vessel to another and from one species to anot...

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Section: Study Of Oxidative Stress Markersmentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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“…Terzano et al 2010. Under physiological conditions, the normal production of reactive oxygen species (ROS) is counteracted by antioxidant scavengers and enzymes, which include both enzymes and non-enzymatic scavengers (de Kok et al 2006 ;Garcon et al 2006 ;Sun et al 2008 ). However, under abnormal conditions, excessive levels of ROS exceed the detoxification capacity of the antioxidant defences, thereby causing a change in the redox status of the cell (Jomova and Valko 2011 ;Lonkar and Dedon 2011 ;Ziech et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Alessandria¹,

Schilirò²,

Degan³

et al. 2014

Environ Sci Pollut Res

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“…Exposure to VOCs may cause upper and lower respiratory symptoms and contribute to the worsening of asthma. The mechanisms of VOC toxicity, however, have yet to be established, even though some experimental evidence supports oxidative stress having a role [1][2][3].…”

mentioning

confidence: 99%

Exposure to volatile organic compounds and loss of pulmonary function in the elderly

Yoon

Hong²,

Cho³

et al. 2010

Eur Respir J

119

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Volatile organic compounds (VOCs) are reported to cause adverse effects on pulmonary function in occupationally exposed workers. However, evidence is lacking on the effect in the general population. We hypothesised that VOCs impair pulmonary function through enhancing oxidative stress, especially in the elderly population.A longitudinal panel study of 154 elderly people was performed in South Korea. Repeated spirometric tests were performed up to eight times on different days for each subject. We also measured urinary concentrations of metabolites of the VOC and markers of oxidative stress (malondialdehyde and 8-oxo-29-deoxyguanosine) on the same day of spirometric tests. A mixed linear regression model was used to evaluate the association among the VOC metabolites, oxidative stress markers and spirometric tests.We found that the urinary levels of hippuric acid and methylhippuric acid, which are metabolites of toluene and xylene, respectively, were significantly associated with reduction of forced expiratory volume in 1 s (FEV1), FEV1/forced vital capacity (FVC), and forced expiratory flow at 25-75% of FVC. We also found significant associations between the metabolites of VOCs and the markers of oxidative stress. In addition, the oxidative stress markers were associated with pulmonary function parameters.This study suggests that exposure to toluene and xylene exert a harmful effect on pulmonary function by exacerbating oxidative stress in elderly people.

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Dunkerque City air pollution particulate matter-induced cytotoxicity, oxidative stress and inflammation in human epithelial lung cells (L132) in culture

Cited by 119 publications

References 47 publications

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Exposure to volatile organic compounds and loss of pulmonary function in the elderly

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