Duplication and subsequent functional diversification of aquaporin family in Pacific abalone Haliotis discus hannai

Jia, Yanglei; Xu, Fei; Xiao, Lianchun

doi:10.1016/j.ympev.2022.107392

Cited by 4 publications

(3 citation statements)

References 76 publications

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“…While aquaporin genes are plentiful in teleosts, it is noteworthy that aqp2, aqp5, and aqp6 are conspicuously absent. These aquaporins have a vital function in safeguarding water balance in amphibians, sauropods, and mammals [19,21]. Consequently, it is theorized by scholars that the absence of aqp2, 5, 6 in teleosts may be attributed to the evolutionary adaptation of tetrapods from sarcopterygian ancestors.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Wang

Zhao

Wang

et al. 2023

IJMS

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The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Wang

Zhao

Wang

et al. 2023

IJMS

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“…However, similar permeation properties were not detected in molluscs. For instance, previous research revealed that although these replacements enlarged the diameter of the AQP ortholog in Pacific abalone, it was still not capable of mediating the permeation of glycerol [ 40 ]. Besides, similar orthologs in terrestrial snail Helix pomatia could stimulate the permeation of hydrogen peroxide but is not permeable to glycerol [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Remarkably, some AQPs with the similar composition pattern of the Ar/R region to that in Mglp also appeared in holometabolan insects to compensate for the absence of Glp subfamily in these species [ 26 ]. Previous research has revealed that the Mglps in Pacific abalone were derived from tandem duplication and subsequent functional differentiation [ 40 ]. However, similar phenomenon was not detected between the tandem duplicated AQP orthologs in oysters, although the Glp subfamily in these species were extremely contracted.…”

Section: Discussionmentioning

confidence: 99%

Diversification of the aquaporin family in geographical isolated oyster species promote the adaptability to dynamic environments

Jia

Liu

2022

BMC Genomics

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Background The diversified aquaporin (AQP) family that was derived from gene duplication and subsequent functional differentiation play critical roles in multiple physiological processes and in adaptation to the dynamic environments during the evolutionary process. Oysters are a group of bivalve fauna in Mollusca that were widely distributed around the world and show extraordinary adaptation to harsh environments. However, knowledge is lacking with the diversity and evolution of the AQP family in oysters, even in molluscs. Results Here, we performed a comprehensive analysis of the AQP family in three geographical isolated oyster species that are native to different environments. Genome distribution and phylogenetic analysis revealed that the expansion of the AQP family in oysters were attributed to tandem duplication. Synteny analysis indicated that large-scale inversions lead to the independent duplication or deletion of the AQPs after speciation. As a consequence, these independent duplication events contributed to the diversification of the AQP family in different oysters. Pore pattern analysis suggested that the duplicated AQPs in oysters were highly diversified in inner surface profiles, implying the subsequent functional differentiation. The comparison conducted based on the transcriptome data demonstrated that the functional differentiated AQP family members in oysters may play critical roles in maintaining the balance between the stationary homeostasis and dynamic environments. Conclusions Our observation provides evidence for the correlation between the duplicated and functional differentiated AQP family and the adaptation to stationary life under dynamic environments in oysters. Additionally, it also broadens our knowledge of the evolution of AQP family in molluscs.

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Artemia enrichment as modulator of gene expression patterns of Enteroctopus megalocyathus paralarvae at pre-settlement stage

Uriarte,

Enríquez,

Hernández

et al. 2024

Aquaculture

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Duplication and subsequent functional diversification of aquaporin family in Pacific abalone Haliotis discus hannai

Cited by 4 publications

References 76 publications

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Diversification of the aquaporin family in geographical isolated oyster species promote the adaptability to dynamic environments

Artemia enrichment as modulator of gene expression patterns of Enteroctopus megalocyathus paralarvae at pre-settlement stage

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