Duplication of blaCTX-M-1 and a class 1 integron on the chromosome enhances antimicrobial resistance in Escherichia coli isolated from racehorses in Japan

Tamamura-Andoh, Yukino; Niwa, Hideaki; Kinoshita, Yuta; Uchida‐Fujii, Eri; Arai, Nobuo; Watanabe-Yanai, Ayako; Iwata, Tadahisa; Akiba, Masato; Kusumoto, Masahiro

doi:10.1016/j.jgar.2021.10.004

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Our data indicate that tandem duplication of the same transposon is also a quite common transposition event as observed in three out of four cases, i.e., in pairs I, III, and IV. In accordance with this observation, a few cases of IS 26 -mediated ESBL gene duplications were described before ( Garza-Ramos et al., 2009 ; Tamamura-Andoh et al., 2021 ; van Almsick et al., 2022 ), and also the association of IS 26 and bla CTX-M genes was already described in Enterobacteriaceae in a systematic study ( Shropshire et al., 2022 ). Interestingly, neither documented IS 26 transposon with ESBL genes nor a high sequence similarity could be found in the databases of TnCentral ( Ross et al., 2021 ) or Transposon Registry ( Tansirichaiya et al., 2019 ) yet.…”

Section: Discussionsupporting

confidence: 79%

“…In addition, we hypothesize that the increased resistance level to ceftazidime correlates with the number of ESBL gene copies encoded on the plasmid. Only very few reported cases of ESBL gene duplications found this correlation between genotypic and phenotypic resistance so far ( Tamamura-Andoh et al., 2021 ; van Almsick et al., 2022 ). However, in accordance, this observation has been described before for other AMR genes ( Coppi et al., 2020 ; Han et al., 2021 ).…”

Section: Discussionmentioning

confidence: 94%

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Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates

Sobkowiak,

Scherff,

Schuler

et al. 2024

Front. Cell. Infect. Microbiol.

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IntroductionThe emergence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is an urgent and alarming One Health problem. This study aimed to investigate duplications of plasmid-encoded ESBL genes and their impact on antimicrobial resistance (AMR) phenotypes in clinical and screening isolates.MethodsMulti-drug-resistant bacteria from hospitalized patients were collected during routine clinical surveillance from January 2022 to June 2023, and their antimicrobial susceptibility patterns were determined. Genotypes were extracted from long-read whole-genome sequencing data. Furthermore, plasmids and other mobile genetic elements associated with ESBL genes were characterized, and the ESBL genes were correlated to ceftazidime minimal inhibitory concentration (MIC).ResultsIn total, we identified four cases of plasmid-encoded ESBL gene duplications that match four genetically similar plasmids during the 18-month surveillance period: five Escherichia coli and three Klebsiella pneumoniae isolates. As the ESBL genes were part of transposable elements, the surrounding sequence regions were duplicated as well. In-depth analysis revealed insertion sequence (IS)-mediated transposition mechanisms. Isolates with duplicated ESBL genes exhibited a higher MIC for ceftazidime in comparison to isolates with a single gene copy (3–256 vs. 1.5–32 mg/L, respectively).ConclusionESBL gene duplications led to an increased phenotypic resistance against ceftazidime. Our data suggest that ESBL gene duplications by an IS-mediated transposition are a relevant mechanism for how AMR develops in the clinical setting and is part of the microevolution of plasmids.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 94%

Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates

Sobkowiak,

Scherff,

Schuler

et al. 2024

Front. Cell. Infect. Microbiol.

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“…Enterobacteriaceae are widely distributed in nature; have a broad host range; and are parasitic or symbiotic in humans, animals, and plants. Some genera in the family Enterobacteriaceae, such as Escherichia ( 51 ), Salmonella ( 52 ), Shigella ( 53 ), and Klebsiella ( 54 ), belong to the common opportunistic pathogens. Ruminococcaceae are the main microorganisms that convert primary bile acids into secondary bile acids and are also involved in polysaccharide degradation ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Luo

et al. 2022

Front. Vet. Sci.

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The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota.

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Whole-genome sequencing analysis of a rare Salmonella diarizonae clinical strain carrying multiple plasmids and novel gene cassettes

Zhao

et al. 2022

Journal of Global Antimicrobial Resistance

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Duplication of blaCTX-M-1 and a class 1 integron on the chromosome enhances antimicrobial resistance in Escherichia coli isolated from racehorses in Japan

Cited by 6 publications

References 18 publications

Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates

Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Whole-genome sequencing analysis of a rare Salmonella diarizonae clinical strain carrying multiple plasmids and novel gene cassettes

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