Dying under pressure: cellular characterisation and <i>in vivo</i> functions of cell death induced by compaction

Valon, Léo; Levayer, Romain

doi:10.1111/boc.201800075

Cited by 36 publications

(27 citation statements)

References 104 publications

(183 reference statements)

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“…Enveloped biologics such as viruses, vaccines and cells are usually the main components sensitive to mechanical destruction. Valon et al studied the possible effects of mechanical stresses on various types of cells, and reported that shear stresses and compaction may lead to apoptosis and cell death [73]. Choi et al also found that hyperosmotic pressure can destroy virus integrity in an acidic environment [32].…”

Section: Challenges Associated With Oral Deliverymentioning

confidence: 99%

Challenges and Recent Progress in Oral Drug Delivery Systems for Biopharmaceuticals

2019

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Routes of drug administration and the corresponding physicochemical characteristics of a given route play significant roles in therapeutic efficacy and short term/long term biological effects. Each delivery method has favorable aspects and limitations, each requiring a specific delivery vehicles design. Among various routes, oral delivery has been recognized as the most attractive method, mainly due to its potential for solid formulations with long shelf life, sustained delivery, ease of administration and intensified immune response. At the same time, a few challenges exist in oral delivery, which have been the main research focus in the field in the past few years. The present work concisely reviews different administration routes as well as the advantages and disadvantages of each method, highlighting why oral delivery is currently the most promising approach. Subsequently, the present work discusses the main obstacles for oral systems and explains the most recent solutions proposed to deal with each issue.

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Section: Challenges Associated With Oral Deliverymentioning

confidence: 99%

Challenges and Recent Progress in Oral Drug Delivery Systems for Biopharmaceuticals

2019

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“…This mechanical competition was described as a new and alternative mode of cell competition and may be relevant in a wide range of genetic and tissue backgrounds. Recent reviews have already discussed the process of mechanical-based competition [59][60][61] and the role of mechanical forces in death induction [62]. We will therefore give a rather brief overview here of the concept of mechanical competition by describing first how cell deformation can trigger cell elimination, then focusing on two alternative modes of compaction-driven elimination.…”

Section: Regulation Of Cell Death By Mechanical Inputs and Mechanical Cell Competitionmentioning

confidence: 99%

Multiple Influences of Mechanical Forces on Cell Competition

Matamoro‐Vidal

Levayer

2019

Current Biology

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Cell competition is a widespread process leading to the expansion of one cell population through the elimination and replacement of another. A large number of genetic alterations can lead to either competitive elimination of the mutated population or expansion of the mutated cells through the elimination of the neighbouring cells. Several processes have been proposed to participate in the preferential elimination of one cell population, including competition for limiting extracellular pro-survival factors, communication through direct cell-cell contact, or differential sensitivity to mechanical stress. Recent quantitative studies of cell competition have also demonstrated the strong impact of the shape of the interfaces between the two populations. Here, we discuss the direct and indirect contribution of mechanical cues to cell competition, where they act either as modulators of competitive interactions or as direct drivers of cell elimination. We first discuss how mechanics can regulate contact-dependent and diffusion-based competition by modulating the shape of the interface between the two populations. We then describe the direct contribution of mechanical stress to cell elimination and competition for space. Finally, we discuss how mechanical feedback also influences compensatory growth and triggers preferential expansion of one population.

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“…This data was consistent with the T D as aforementioned. It has been evidenced that cell deformability is associated with cytotoxicity and cell death, and the late stage of cell deformation can lead to a loss of the cell membrane and ultimately cellular disruption and death 43 , 44 . On the other hand, the protein-free AU-Siriraj formula did not induce the cell death, consistent with our previous study demonstrating that this AU formula is quite physiologic 1 .…”

Section: Discussionmentioning

confidence: 99%

Optimization of artificial urine formula for in vitro cellular study compared with native urine

Sueksakit¹,

Thongboonkerd²

2021

Int. J. Med. Sci.

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Several artificial urine (AU) formulas have been developed to mimic the normal urine. Most of them are protein-free, particularly when secreted proteins (secretome) is to be analyzed. However, the normal urine actually contains a tiny amount of proteins. We hypothesized that urinary proteins at physiologic level play a role in preservation of renal cell biology and function. This study evaluated the effects from supplementation of 0-10% fetal bovine serum (FBS) into the well-established AU-Siriraj protocol on MDCK renal tubular cells. Time to deformation (T D ) was reduced by both native urine and AU-Siriraj without/with FBS compared with complete culture medium (control). Among the native urine and AU-Siriraj without/with FBS, the cells in AU-Siriraj+2.5% FBS had the longest T D . Supplementation of FBS increased cell death in a dose-dependent manner (but still <10%). Transepithelial electrical resistance (TER) of the polarized cells in the native urine was comparable to the control, whereas that of the cells in AU-Siriraj+2.5% FBS had the highest TER. These data indicate that supplementation of 2.5% FBS into AU-Siriraj can prolong time to deformation and enhance polarization of renal tubular cells. Therefore, AU-Siriraj+2.5% FBS is highly recommended for in vitro study of cell biology and function (when secretome is not subjected to analysis).

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Dying under pressure: cellular characterisation and in vivo functions of cell death induced by compaction

Cited by 36 publications

References 104 publications

Challenges and Recent Progress in Oral Drug Delivery Systems for Biopharmaceuticals

Challenges and Recent Progress in Oral Drug Delivery Systems for Biopharmaceuticals

Multiple Influences of Mechanical Forces on Cell Competition

Optimization of artificial urine formula for in vitro cellular study compared with native urine

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