Hill number per unit area and seedling number per hill are foundational agrotechnical factors shaping the growth and development of rice plants. This study aimed to determine the effects of decreasing the hill number per unit area combined with increasing the seedling number per hill on grain quality in hybrid rice. Field experiments were performed in Hengyang, Hunan Province, China, in 2022 and 2023 using the hybrid rice variety Huazheyou 261, as well as in Liuyang, Hunan Province, China, in 2023 using the hybrid rice varieties Yueyou 2646 and Zhenliangyouyuzhan. Treatments (combining hill number per unit area and seedling number per hill) encompassed a combination of 24 hills per m2 and one seedling per hill (H24S1) and a combination of 14 hills per m2 and three seedlings per hill (H14S3) in Hengyang, as well as a combination of 28 hills per m2 and two seedlings per hill (H28S2) and a combination of 14 hills per m2 and four seedlings per hill (H14S4) in Liuyang. There were no significant differences in the leaf area index at the heading stage, as well as no significant differences in canopy light transmittance during the grain-filling period between H24S1 and H14S3 in Hengyang, or between H28S2 and H14S4 in Liuyang. The differences in grain quality traits, including milling traits (brown, milled, and head rice rate), appearance traits (rice length, rice length-width ratio, chalky grain rate, and chalkiness degree), amylose and protein content, and pasting characteristics were also not significant between H24S1 and H14S3 in Hengyang, nor between H28S2 and H14S4 in Liuyang. This study indicates that the grain quality in hybrid rice is unaffected by decreasing the hill number per unit area integrated with increasing the seedling number per hill.