Dynamic Changes in Seed Germination under Low-Temperature Stress in Maize

Abstract

Low-temperature stress delays seed germination in maize. Different maize inbred lines display various low-temperature resistance, but the dynamic changes in seed germination under low-temperature stress in maize remain unknown, especially at the transcriptome level. In this study, low-temperature-resistant maize (RM) inbred line 04Qun0522-1-1 had a significantly faster germination speed than low-temperature-sensitive maize (SM) line B283-1 under low-temperature stress. Moreover, the total antioxidant capacity, superoxide dismutase, and peroxidase activities were notably higher in the RM line than in the SM line from 3 to 6 d. In contrast, the SM line showed significantly higher malondialdehyde (MDA) content than the RM line at 6 d. Gene ontology (GO) enrichment analysis showed that in 2dvs0d, both SM and RM lines displayed the downregulation of ribosome-related genes. Moreover, photosystem II and heat shock protein binding-related genes were also downregulated in the SM line. In 4dvs2d, the RM line showed a higher degree of upregulation of the ribosome and peroxidase (POD)-related genes than the SM line. In 6dvs4d, POD-related genes were continuously upregulated in both SM and RM lines, but the degree of upregulation of the genes was higher in the SM line than in the RM line. Moreover, vitamin B6-related genes were specifically upregulated in the RM line. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that in 6dvs4d, phenylpropanoid biosynthesis was the most significantly enriched pathway in both SM and RM lines. Moreover, phenylpropanoid biosynthesis was also enriched in the RM line in 4dvs2d. More than half of the differentially expressed genes (DEGs) in phenylpropanoid biosynthesis were peroxidase, and the DEGs were similar to the GO enrichment analysis. The results provide new insights into maize seed germination in response to low-temperature stress.

Construction and Identification of Cold Tolerance in Different Broccoli Cultivars at the Seedling Stage

“…In general, cold stress can induce membrane lipid peroxidation in plants and produce the accumulation of membrane lipid peroxidation, MDA, which is directly proportional to the stress time and inversely proportional to the temperature [38]. In this study, there was a significant increase in MDA in different broccoli genotypes at the seedling stage compared to that in the control after cold stress, which is consistent with some previous research [39].…”

Integrated metabolomics and transcriptomics analysis during seed germination of waxy corn under low temperature stress

“…Low temperature stress can affect a series of morphological and physiological changes of plants, and delay seed germination of maize, which has a great impact on its growth, yield and quality [ 38 ]. LT stress can increase the accumulation of reactive oxygen species (ROS), and the ROS scavenging enzymes in plants are mainly antioxidant enzymes, such as SOD, POD and CAT, etc [ 39 ].…”

Transcriptomic Profiling of Two Rice Thermo-Sensitive Genic Male Sterile Lines with Contrasting Seed Storability after Artificial Accelerated Aging Treatment

“…Ribosomes are indispensable components of cells that translate mRNA into functional proteins [11]. Endogenous signals and environmental stimuli, such as ambient temperature, can regulate ribosome biosynthesis [12][13][14]. Previous studies have identified that low temperature rapidly inhibits ribosomal biogenesis [15].…”