Dynamic changes of gut microbiota of discus fish (Symphysodon haraldi) at different feeding stages

Zhang, Yuan; Wen, Bin; Meng, Liu-Jiang; Gao, Jian‐Zhong

doi:10.1016/j.aquaculture.2020.735912

Cited by 27 publications

(12 citation statements)

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“…Moreover, predominant microbiota was closely related to the intestinal environment and may have some special functions (Zhao et al, 2020). Recently, PICRUSt1 was widely performed to predict microbiota function (Yang et al, 2019; Zhang et al, 2021). In this study, we used PICRUSt1 to predict the COG function of fish gut microbiota and found many microbial functional genes related to host amino acid transport and metabolism, energy production and conversion, and transcription and carbohydrate transport and metabolism, indicating that gut microbiota exerts some influence on the host metabolism, consistent with previous studies (Li et al, 2018; Ma et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Hybridization exerts some influences on gut bacteria, and the changes in the hybrids' gut microbiota may affect gene expression and fitness of the host (Malukiewicz et al, 2022), and lead to favourable physiological functions in some cases (Seehausen et al, 2014). However, PICRUSt, as a functional prediction tool, should be interpreted with caution when applied to fish rather than human microbial ecology due to the lack of representation of fishassociated microorganisms in genomic databases (Zhang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Gut microbiota communities of reciprocal hybrids from koi (Cyprinus carpio) and goldfish (Carassius auratus) are more similar to koi than goldfish

Wang

Liu

Wen

et al. 2022

Journal of Applied Microbiology

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Aims To investigate the gut microbiota communities of reciprocal hybrids and inbred lines of koi (Cyprinus carpio) and goldfish (Carassius auratus), as well as the genetic effect of intestinal microbiota between hybrids and parents. Methods and Results The reciprocal hybrids and inbred lines derived from the parents, koi and goldfish, were established. Then, the bacterial 16S rRNA gene of intestinal contents was sequenced using Illumina Miseq PE300. Alpha diversity in the two types of hybrids was lower than inbred lines of koi or goldfish and was highest in goldfish, followed by koi. For beta diversity, microbial samples presented clear clusters and the two types of hybrids were more similar to koi than goldfish, indicating the gut microbiota of the reciprocal hybrids was more affected by koi. The dominant phyla were Proteobacteria, Actinobacteria and Firmicutes in koi, and Proteobacteria, Fusobacteria and Actinobacteria in goldfish, and Proteobacteria, Fusobacteria and Firmicutes in the reciprocal hybrids. In the case of Proteobacteria, the dominant classes were Alphaproteobacteria and Gammaproteobacteria in four fish. The dominant genera were norank_f_Rhizobiales_Incertae_Sedis and Plesiomonas in koi, Cetobacterium in goldfish, and Cetobacterium and ZOR0006 in the reciprocal hybrids. PICRUSt1 predictive function analysis showed that the reciprocal hybrids had lower abundance in the most functional categories than koi and goldfish. Conclusions The gut microbiota of reciprocal hybrids was more affected by koi. Two types of hybrids possessed the same dominated phyla and were different from the inbred lines of koi and goldfish. Significance and Impact of the Study It enhanced our understanding of gut microbiota of hybrid lines of goldfish and koi and provided a new perspective for the selective breeding of gut microbiota traits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gut microbiota communities of reciprocal hybrids from koi (Cyprinus carpio) and goldfish (Carassius auratus) are more similar to koi than goldfish

Wang

Liu

Wen

et al. 2022

Journal of Applied Microbiology

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Section: Introductionmentioning

confidence: 99%

“…Age is closely related to the intestinal microbiota of animals. The reorganization of intestinal microbiotas caused by aging is primarily related to changes in the structure and physical/chemical properties of the digestive system (Bonder et al, 2016; Chen, Cui, et al, 2021; Chen, Li, et al, 2021; Vangay et al, 2018; Zhang et al, 2021). For instance, the stripping and shedding of old intestines during the metamorphic development of some mosquitoes can lead to complete or nearly complete elimination of intestinal bacteria, resulting in the absence of bacteria in the intestines of newly emerged adults (Moll et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

Yang

Cui

Ning

et al. 2022

Ecology and Evolution

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Intestinal microbiota play an important role in the life of amphibians and its composition may vary by developmental stage. In this study, 16S rRNA high‐throughput sequencing was used to profile the intestinal microbiota of Hynobius maoershanensis, which exclusively inhabit the Maoer Mountain swamp at an altitude of approximately 2,000 m. We characterized the bacterial composition, structure, and function of the microbiota of H. maoershanensis at different developmental stages. The alpha diversity was not markedly different for the Simpson, Shannon, Ace, and Sobs indices of microbes. The beta diversity revealed that there were age‐related differences in the structure of the intestinal microbes of H. maoershanensis, specifically, at the phylum level. Bacteroidetes and Proteobacteria were the dominant bacteria present in the adult stage, and the relative abundance of Bacteroidetes was significantly higher compared with that of tadpoles. Firmicutes and Proteobacteria were the dominant phylum during the tadpole stage and their relative abundance was significantly higher compared with the adult period. Functional analysis revealed that the pathways associated with organismal systems and metabolism were significantly enriched in the adults, whereas human diseases, genetic information processing, and cellular processes were more enriched in the hindlimb bud stage. Human diseases and environmental information processing were more enriched in the forelimb bud stage at KEGG pathway level 1. Possibilities for the observed discrepancies include the adaptation to eating habits and the remodeling of the intestines during development. We speculated that H. maoershanensis adults may be more suitable to a high‐fiber diet, whereas the tadpoles are associated with a carnivorous diet. Our study provides evidence of variations in the intestinal microbiota during development in amphibians, highlighting the influence of historical developments on the intestinal microbiota and an increased understanding of the importance of physiological characteristics in shaping the intestinal microbiota of amphibians. These data will help us formulate more effective protection measures for H. maoershanensis.

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“…At last, colonization continues throughout life by microbes from water and food. Changes in diet composition or transferring between rearing systems are common practices in aquaculture, which result in significant shifts in gut microbiota as the fish grows (Zhang et al, 2021). Therefore, the changes of nutrients availability as well as gut functionality during development contribute to the temporal variation of gut microbiota.…”

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confidence: 99%

The effect of induced changes in gut microbiota on fish performance

Deng¹

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The establishment of the early-life gut microbiota plays an important role in fish development and influences the health status and growth performance of the host. The effect of different rearing conditions can have an impact on the initial colonization of the gut microbiota, while addition of probiotics may also affect such colonization. However, how this may affect fish larvae survival and growth remains largely unexplored. In this study, 3 day old Nile tilapia embryos were hatched until 9 days post fertilization (dpf) in three systems, including one flowthrough system (FTS) and two identical recirculating aquaculture systems (RAS). When feeding started at 10 dpf, tilapia larvae in the FTS and in one of the RAS were fed with a control diet, while larvae in the second RAS were fed with the control diet coated with B. subtilis spores (RASB). The feeding trial lasted 26 days, from larvae to fry stage, during which the survival, growth performance and gut microbiota were analyzed. The larvae reared in FTS showed significantly lower survival than the ones in RAS and RASB, while no differences were observed in fish growth and apparent feed conversion ratio between treatments. Different rearing systems resulted in different gut bacterial compositions which strongly correlated with the survival rate and standard body length at harvest. Cetobacterium was enriched in RAS and RASB, while it was barely detected in the gut of FTS-reared tilapia fry. Probiotic supplementation increased the relative abundance of beneficial Bacillus in fish gut. Our findings indicate that rearing fish larvae in RAS supports better survival compared to FTS, while dietary probiotic supplementation further modulates the gut bacterial composition and stimulates presence of beneficial bacteria during early life.

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Dynamic changes of gut microbiota of discus fish (Symphysodon haraldi) at different feeding stages

Cited by 27 publications

References 50 publications

Gut microbiota communities of reciprocal hybrids from koi (Cyprinus carpio) and goldfish (Carassius auratus) are more similar to koi than goldfish

Gut microbiota communities of reciprocal hybrids from koi (Cyprinus carpio) and goldfish (Carassius auratus) are more similar to koi than goldfish

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

The effect of induced changes in gut microbiota on fish performance

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