Dynamic distribution and tissue tropism of nervous necrosis virus in juvenile pompano (Trachinotus ovatus) during early stages of infection

Su, Youlu; Xu, Huailiang; Ma, Hongling; Jiang, Feng; Wang, Wen; Guo, Zhiyong

doi:10.1016/j.aquaculture.2015.01.033

Cited by 32 publications

(9 citation statements)

References 27 publications

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“…The dynamics of NNV infections seem to be fast; the RGNNV genome was detected in the brain of infected pompano at 4 hpi [188] and both genome and infective particles of a reassortant RGNNV/SJNNV strain were detected after 1 day post infection (dpi) in the brain of Senegalese sole [159]. However, differences related to genotypes and fish species can be observed because in Senegalese sole the RGNNV genome was not detected until 3 dpi, and the detection of infective particles took one more day (4 dpi) [159].…”

Section: Routes Of Infection and Spread Through The Fish Bodymentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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Section: Routes Of Infection and Spread Through The Fish Bodymentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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“…NNV infection is characterized histopathologically by vacuolation in the retinal and brain tissues (Mori et al, ). RGNNV has been detected in the brain of T. ovatus and thus was chosen as a fish model to research the epidemiological characterization of NNV (Xu et al, ; Ma et al, ; Su et al, ).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a new fish cell line from the caudal fin of golden pompano Trachinotus ovatus and its susceptibility to iridovirus

Wei

Qin

2018

Journal of Fish Biology

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A new cell line derived from the caudal fin of golden pompano Trachinotus ovatus (TOCF) was successfully established and characterized. TOCF cells grew well at 28° C in L-15 medium supplemented with 10% foetal bovine serum (FBS). The cell line has been subcultured in more than 100 passages. Molecular characterization of 18S ribosomal (r)RNA and cytochrome oxidase subunit 1 (COI) confirmed that the TOCF cells were derived indeed from T. ovatus. TOCF cells have a modal chromosome number of 54. It was further showed that TOCF cells were transfected successfully with pEGFP-N3 and pDsRED-N1 plasmid, suggesting that TOCF cells could be used to research gene functions in vitro. Viral susceptibility tests showed that TOCF cells were susceptible to Singapore grouper iridovirus (SGIV), observed by the occurrence of the cytopathic effect (CPE) with the formation of inclusion bodies. In addition, the expression of major capsid protein (MCP) gene of SGIV changed during virus infection in TOCF cells. Thus, our present results described the characteristic of a TOCF cell line that could be a valuable tool for genetic manipulation, as well as isolation and propagation of iridovirus studies.

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“…In recent years, golden pompano industries developed rapidly and it became the main species of cage culture. Along with the expansion, T. ovatus aquaculture industries worldwide have suffered viral diseases outbreaks what caused serious economic losses (Xu et al 2010, Su et al 2015. Recently, qRT-PCR has become a widely used method of investigating gene expression in golden pompano.…”

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confidence: 99%

Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

Chen¹,

Zhang²,

Huang³

et al. 2017

Polish Journal of Veterinary Sciences

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Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes. When studying gene expression under conditions of poly

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Dynamic distribution and tissue tropism of nervous necrosis virus in juvenile pompano (Trachinotus ovatus) during early stages of infection

Cited by 32 publications

References 27 publications

Betanodavirus and VER Disease: A 30-year Research Review

Betanodavirus and VER Disease: A 30-year Research Review

Establishment of a new fish cell line from the caudal fin of golden pompano Trachinotus ovatus and its susceptibility to iridovirus

Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

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