Dynamic drug release state and PEG length in PEGylated liposomal formulations define the distribution and pharmacological performance of drug

Lim, Chaemin; Shin, Yuseon; Lee, Sehwa; Lee, Subin; Lee, Moo-Yeol; Shin, Beom Soo; Oh, Kyung Taek

doi:10.1016/j.jddst.2022.103825

Cited by 4 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were washed and starved in serum-free DMEM for 1 h. After, cells were treated with various concentrations of peptides for 24 h. The CCK-8 reagent was added to each well and incubated for an additional 4 h. The absorbance was measured at 450 nm using a SpectraMax ABS Plus Microplate reader (Molecular Devices, USA). [40][41][42] 2.7. Enzyme-linked immunosorbent assay (ELISA) RAW 264.7 cells were evaluated for cytokine inhibition mediated by peptides and the formulated form.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Development and application of novel peptide-formulated nanoparticles for treatment of atopic dermatitis

Lim,

Lee,

Shin

et al. 2023

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Cell Viability Assaymentioning

confidence: 99%

Development and application of novel peptide-formulated nanoparticles for treatment of atopic dermatitis

Lim,

Lee,

Shin

et al. 2023

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…Considering that the EPR effect is highly dependent on the circulation time of liposomes in the bloodstream, modifications to the surface of liposomes have been proposed to enhance their circulation time [ 24 ]. The covalent linking of polyethylene glycol (PEG) chains to liposomes’ surface, known as PEGylation, is the most used approach to obtain sterically stabilized liposomes (stealth liposomes) [ 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetoliposomes with Calcium-Doped Magnesium Ferrites Anchored in the Lipid Surface for Enhanced DOX Release

Cardoso,

Fernandes,

Amorim

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

Nanotechnology has provided a new insight into cancer treatment by enabling the development of nanocarriers for the encapsulation, transport, and controlled release of antitumor drugs at the target site. Among these nanocarriers, magnetic nanosystems have gained prominence. This work presents the design, development, and characterization of magnetoliposomes (MLs), wherein superparamagnetic nanoparticles are coupled to the lipid surface. For this purpose, dimercaptosuccinic acid (DMSA)-functionalized Ca0.25Mg0.75Fe2O4 superparamagnetic nanoparticles were prepared for the first time. The magnetic nanoparticles demonstrated a cubic shape with an average size of 13.36 nm. Furthermore, their potential for photothermal hyperthermia was evaluated using 4 mg/mL, 2 mg/mL, and 1 mg/mL concentrations of NPs@DMSA, which demonstrated a maximum temperature variation of 20.4 °C, 11.4 °C, and 7.3 °C, respectively, during a 30 min NIR-laser irradiation. Subsequently, these nanoparticles were coupled to the lipid surface of DPPC/DSPC/CHEMS and DPPC/DSPC/CHEMS/DSPE-PEG-based MLs using a new synthesis methodology, exhibiting average sizes of 153 ± 8 nm and 136 ± 2 nm, respectively. Doxorubicin (DOX) was encapsulated with high efficiency, achieving 96% ± 2% encapsulation in non-PEGylated MLs and 98.0% ± 0.6% in stealth MLs. Finally, drug release assays of the DOX-loaded DPPC/DSPC/CHEMS MLs were performed under different conditions of temperature (37 °C and 42 °C) and pH (5.5 and 7.4), simulating physiological and therapeutic conditions. The results revealed a higher release rate at 42 °C and acidic pH. Release rates significantly increased when introducing the stimulus of laser-induced photothermal hyperthermia at 808 nm (1 W/cm2) for 5 min. After 48 h of testing, at pH 5.5, 67.5% ± 0.5% of DOX was released, while at pH 7.4, only a modest release of 27.0% ± 0.1% was achieved. The results demonstrate the potential of the MLs developed in this work to the controlled release of DOX under NIR-laser stimulation and acidic environments and to maintain a sustained and reduced release profile in physiological environments with pH 7.4.

show abstract

“…Because of its versatility and excellent biocompatibility, PEG macromer chemistry has promoted the development of numerous intelligently designed hydrogel systems [ 20 ]. For example, by using PEG as a matrix suppository, irregular movements of drugs in water can be avoided, while drugs can also be released quickly after contacting the water [ 21 , 22 , 23 ]. Inspired by the suppository prescription, PEG was employed to solidify the nucleic acid preparation particles in this work, so that the viscosity index η could increase infinitely, thus reducing the Brownian motion displacement in solution.…”

Section: Introductionmentioning

confidence: 99%

PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations

Cui

Jiang

et al. 2022

Gels

View full text Add to dashboard Cite

Currently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high cost and inconvenient operatios. In this study, the hydrophilic pharmaceutical excipient PEG was used to gelate nucleic acid preparations to avoid the random movements of liquid particles. The results showed that PEG gelation significantly improved the stability of PEI25K−based and liposome−based nucleic acid preparations, compared with nucleic acid preparations without PEG gelation. After being stored at 4 °C for 3 days, non−PEG gelled nucleic acid preparations almost lost transfection activity, while PEGylated preparations still maintained high transfection efficiency. Fluorescence experiments showed that this effect was caused by inhibiting particle aggregation. The method described in this study was simple and effective, and the materials used had good biocompatibility. It is believed that this study will contribute to the better development of gene therapy drugs.

show abstract

Dynamic drug release state and PEG length in PEGylated liposomal formulations define the distribution and pharmacological performance of drug

Cited by 4 publications

References 55 publications

Development and application of novel peptide-formulated nanoparticles for treatment of atopic dermatitis

Development and application of novel peptide-formulated nanoparticles for treatment of atopic dermatitis

Magnetoliposomes with Calcium-Doped Magnesium Ferrites Anchored in the Lipid Surface for Enhanced DOX Release

PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations

Contact Info

Product

Resources

About