Dynamic evolution of NBS–LRR genes in bread wheat and its progenitors

Gu, Ling; Si, Weina; Zhao, Lina; Yang, Sihai; Zhang, Xiaohui

doi:10.1007/s00438-014-0948-8

Cited by 56 publications

(48 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Focus should be diverted toward identification of more Pst resistance genes from wild relatives. More than 2,500 members of the NBS-LRR gene family have been reported in wheat, 570 in Triticum urata , 842 in Aegilops tauschii , 316 in Brachypodium distachyon , and 420 in Hordeum vulgare ( 79 ), and majority of these have potential for disease resistance ( 80 ). Use of bioinformatics tools will help to understand the functions of the NBS-LRR gene family for hunting putative Pst resistance genes.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

View full text Add to dashboard Cite

Puccinia striiformis (Pst) is a devastating biotrophic fungal pathogen that causes wheat stripe rust. It usually loves cool and moist places and can cause 100% crop yield losses in a single field when ideal conditions for disease incidence prevails. Billions of dollars are lost due to fungicide application to reduce stripe rust damage worldwide. Pst is a macrocyclic, heteroecious fungus that requires primary (wheat or grasses) as well as secondary host ( Berberis or Mahonia spp.) for completion of life cycle. In this review, we have summarized the knowledge about pathogen life cycle, genes responsible for stripe rust resistance, and susceptibility in wheat. In the end, we discussed the importance of conventional and modern breeding tools for the development of Pst -resistant wheat varieties. According to our findings, genetic engineering and genome editing are less explored tools for the development of Pst -resistant wheat varieties; hence, we highlighted the putative use of advanced genome-modifying tools, i.e., base editing and prime editing, for the development of Pst -resistant wheat.

show abstract

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

View full text Add to dashboard Cite

show abstract

“…We used the leaf rust isolate #526-24 and stripe rust isolate #5006 from the stocks of the Institute for Cereal Crops Improvement. The virulence / avirulence (V / Av) formula of these isolates is Lr1, 3,24,26,10,18,21,23,15 / Lr2a,2c,9,16,3 ka,11,17,30 and Yr6,7,8,9,11,12,17,19,sk,18, A / Yr1,5,10,15,24,26,sp, for isolates #526-24 and #5006, respectively. Both of these isolates were used to select resistant progenies at the seedling stage and to evaluate adult plant resistance.…”

Section: Pathogensmentioning

confidence: 99%

“…To date, more than 70 resistance genes against leaf rust and stripe rust originating in wheat or in its wild relatives have been identified and mapped [9]. Most of these genes confer seedling resistance although adult plant resistance (APR) genes were also identified [10,11].…”

Section: Introductionmentioning

confidence: 99%

Reducing the size of an alien segment carrying leaf rust and stripe rust resistance in wheat

et al. 2020

View full text Add to dashboard Cite

Background: Leaf and stripe rusts are two major wheat diseases, causing significant yield losses. The preferred way for protecting wheat from rust pathogens is by introgression of rust resistance traits from wheat-related wild species. To avoid genetic drag due to replacement of large wheat chromosomal segments by the alien chromatin, it is necessary to shorten the alien chromosome segment in primary recombinants. Results: Here we report on shortening of an alien chromosome segment in wheat that carries leaf and stripe rust resistance from Sharon goatgrass (Aegilops sharonensis). Rust resistant wheat introgression lines were selected and the alien region was mapped using genotyping by sequencing. Single polymorphic nucleotides (SNP) were identified and used to generate diagnostic PCR markers. Shortening of the alien fragment was achieved by induced homoeologous pairing and lines with shortened alien chromosome were identified using the PCR markers. Further reduction of the segment was achieved in tertiary recombinants without losing the rust resistance. Conclusions: Alien chromatin in wheat with novel rust resistance genes was characterized by SNP markers and shortened by homoeologous recombination to avoid deleterious traits. The resulting wheat lines are resistant to highly virulent races of leaf and stripe rust pathogens and can be used as both resistant wheat in the field and source for gene transfer to other wheat lines/species.

show abstract

“…According to the conserved domain in the N terminal, the cloned R genes were classified into two types, one type contains the conserved domain resembling the Drosophila Toll and mammalian IL-1 receptors (TIR) and named as TIR subclass, and the other named as non-TIR class (Meyers et al 1999). In the non-TIR class, most NLR genes contain the coiled-coil (CC) domain, and a few NLR genes contain the leucine zipper motif or RPW8 domain (Gu et al, 2015). The genomic data of a number of species have been sequenced, and it was found that dicot species contained both the TIR class and non-TIR class, while the monocot species contained only the non-TIR class.…”

Section: Introductionmentioning

confidence: 99%

NLR1-V, a CC-NBS-LRR encoding gene, is a potential candidate gene of the wheat powdery mildew resistance genePm21

Xing

Liu

et al. 2017

Preprint

View full text Add to dashboard Cite

Wheat powdery mildew caused by Blumeria graminisb f. sp. tritici is one of the most destructive diseases all over the world. Pm21, transferred from the wild Haynaldia villosa to wheat, confers broad spectrum resistance throughout the whole stage, and this gene has been widely used in wheat production for more than 20 years. Cloning the candidate gene of Pm21 is the prerequisite for elucidating the resistance mechanism, and is a valuable attempt to clone the target genes from the evolutionarily distant wild species. In this study, an innovative approach, which combined cytogenetic stocks development, mutagenesis, RenSeq and PacBio, was tried successfully to clone an NBS-LRR type gene NLR1-V from the Pm21 locus. Firstly, a powdery mildew resistant cryptic alien introgression line HP33 involved very small 6VS segment was developed, and 6 independent susceptible mutants of T6VS· 6AL was identified. Then, the transcriptome of H. villosa was obtained by NGS and the full-length NBS-LRR gene database was constructed by RenSeq-PacBio. In the following study, two expressed NLR genes were located to the Pm21 locus using the HP33 as the mapping material, and only NLR1-V showed polymorphism between the wild T6VS· 6AL and its six mutants. The functional analysis indicated that silencing of NLR1-V could compromise the resistance of T6VS· 6AL completely, and could also decrease the resistance of T6VS· 6DL dramatically. Moreover, NLR1-V could recover the resistance of the susceptible mutant and increase the resistance in the susceptible wheat. The study implied that NLR1-V, a CC-NBS-LRR encoding gene, is a potential candidate gene of the powdery mildew resistance gene Pm21.

show abstract

Dynamic evolution of NBS–LRR genes in bread wheat and its progenitors

Cited by 56 publications

References 62 publications

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

Reducing the size of an alien segment carrying leaf rust and stripe rust resistance in wheat

NLR1-V, a CC-NBS-LRR encoding gene, is a potential candidate gene of the wheat powdery mildew resistance genePm21

Contact Info

Product

Resources

About