Dynamic expression of manganese superoxide dismutase during mouse embryonic organogenesis

Abstract

The balance between reactive oxygen species production and antioxidant defense enzymes in embryos is necessary for normal embryogenesis. To determine the dynamic expression profile of manganese superoxide dismutase (MnSOD) in embryos, which is an essential antioxidant enzyme in embryonic organogenesis, the expression level and distribution of MnSOD mRNA and protein were investigated in mouse embryos, as well as extraembryonic tissues on embryonic days (EDs) 7.5-18.5. MnSOD mRNA levels were remarkably high in extraembryonic tissues rather than in embryos during these periods. MnSOD protein levels were also higher in extraembryonic tissues than in embryos until ED 16.5, but the opposite trend was found after ED 17.5. MnSOD mRNA was observed in the chorion, allantois, amnion, ectoderm, ectoplacental cone and neural fold at ED 7.5 and in the neural fold, gut, ectoplacental cone, outer extraembryonic membranes and primitive heart at ED 8.5. After removing the extraembryonic tissues, the prominent expression of MnSOD mRNA in embryos was seen in the sensory organs, central nervous system and limbs on EDs 9.5-12.5 and in the ganglia, spinal cord, sensory organ epithelia, lung, blood cells and vessels, intestinal and skin epithelia, hepatocytes and thymus on EDs 13.5-18.5. Strong MnSOD immunoreactivity was observed in the choroid plexus, ganglia, myocardium, blood vessels, heapatocytes, pancreatic acinus, osteogenic tissues, brown adipose tissue, thymus and skin. These findings suggest that MnSOD is mainly produced from extraembryonic tissues and then may be utilized to protect the embryos against endogenous or exogenous oxidative stress during embryogenesis.

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Curbing cancer's sweet tooth: Is there a role for MnSOD in regulation of the Warburg effect?

“…Yon et al . (Yon et al, 2011) discovered an interesting dynamic in the expression of MnSOD in both embryonic and extra-embryonic tissues during the mouse gestational period studied (embryonic days (ED) 7.5–18.5). In extra-embryonic tissues, MnSOD expression at the mRNA and protein levels was elevated compared to embryonic tissues.…”

Influx mechanisms in the embryonic and adult rat choroid plexus: a transcriptome study

“…Copper, zinc, and manganese are cofactors of several enzymatic systems, in particular superoxide dismutases (SOD) involved in regulating oxidative stress. Both Mn-SOD and Cu-Zn-SOD are well expressed in choroid plexus throughout development (Yon et al, 2008 , 2011 ). Thus, a primary function of metal transporters in the choroid plexus may be to supply metals required for proper functioning of the blood-CSF barrier.…”